BRENDA - Enzyme Database

Dietary phenolic compounds selectively inhibit the individual subunits of maltase-glucoamylase and sucrase-isomaltase with the potential of modulating glucose release

Simsek, M.; Quezada-Calvillo, R.; Ferruzzi, M.G.; Nichols, B.L.; Hamaker, B.R.; J. Agric. Food Chem. 63, 3873-3879 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.2.1.3
recombinant expression of the glucoamylase subunit Ct-MGAM splice form N20 and the sucrase subunit Ct-SI in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
Mus musculus
3.2.1.48
expression in Drosophila S2 cells; recombinant expression in a baculovirus-Sf9 insect cell system
Homo sapiens
3.2.1.48
expression in Drosophila S2 cells; recombinant expression in a baculovirus-Sf9 insect cell system
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.3
(+)-catechin
noncompetitive inhibition
Mus musculus
3.2.1.3
caffeic acid
noncompetitive inhibition
Mus musculus
3.2.1.3
chlorogenic acid
noncompetitive inhibition
Mus musculus
3.2.1.3
epigallocatechin gallate
EGCG, noncompetitive inhibition
Mus musculus
3.2.1.3
gallic acid
noncompetitive inhibition
Mus musculus
3.2.1.3
additional information
kinetics and mechanism of inhibition of the recombinant glucoamylase subunit Ct-MGAM and sucrase subunit Ct-SI, overview
Mus musculus
3.2.1.48
(+)-catechin
noncompetitive
Homo sapiens
3.2.1.48
(+)-catechin
noncompetitive
Mus musculus
3.2.1.48
(-)-epigallocatechin gallate
noncompetitive
Homo sapiens
3.2.1.48
(-)-epigallocatechin gallate
noncompetitive
Mus musculus
3.2.1.48
caffeic acid
noncompetitive
Homo sapiens
3.2.1.48
caffeic acid
noncompetitive
Mus musculus
3.2.1.48
chlorogenic acid
noncompetitive
Homo sapiens
3.2.1.48
chlorogenic acid
noncompetitive
Mus musculus
3.2.1.48
gallic acid
noncompetitive
Homo sapiens
3.2.1.48
gallic acid
noncompetitive
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.3
membrane
-
Mus musculus
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.3
starch + H2O
Mus musculus
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.3
Mus musculus
Q6XK24
-
-
3.2.1.48
Homo sapiens
P14410
-
-
3.2.1.48
Mus musculus
B5THE3
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.3
recombinant glucoamylase subunit Ct-MGAM splice form N20 and subunit Ct-SI from Spodoptera frugiperda Sf9 cells
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.3
starch + H2O
-
750886
Mus musculus
?
-
-
-
?
3.2.1.3
starch + H2O
soluble starch
750886
Mus musculus
?
-
-
-
?
3.2.1.48
maltose + H2O
-
750886
Homo sapiens
alpha-D-glucose
-
-
-
?
3.2.1.48
maltose + H2O
-
750886
Mus musculus
alpha-D-glucose
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.2.1.3
37
-
assay at
Mus musculus
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.2.1.3
0.00151
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
epigallocatechin gallate
3.2.1.3
0.0017
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
epigallocatechin gallate
3.2.1.3
0.0018
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
chlorogenic acid
3.2.1.3
0.003
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
chlorogenic acid
3.2.1.3
0.0204
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
(+)-catechin
3.2.1.3
0.0216
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
(+)-catechin
3.2.1.3
0.0249
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
gallic acid
3.2.1.3
0.0313
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
gallic acid
3.2.1.3
0.0565
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
caffeic acid
3.2.1.3
0.0582
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
caffeic acid
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.3
recombinant expression of the glucoamylase subunit Ct-MGAM splice form N20 and the sucrase subunit Ct-SI in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
Mus musculus
3.2.1.48
expression in Drosophila S2 cells; recombinant expression in a baculovirus-Sf9 insect cell system
Homo sapiens
3.2.1.48
expression in Drosophila S2 cells; recombinant expression in a baculovirus-Sf9 insect cell system
Mus musculus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.2.1.3
0.00151
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
epigallocatechin gallate
3.2.1.3
0.0017
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
epigallocatechin gallate
3.2.1.3
0.0018
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
chlorogenic acid
3.2.1.3
0.003
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
chlorogenic acid
3.2.1.3
0.0204
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
(+)-catechin
3.2.1.3
0.0216
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
(+)-catechin
3.2.1.3
0.0249
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
gallic acid
3.2.1.3
0.0313
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
gallic acid
3.2.1.3
0.0565
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-MGAM
Mus musculus
caffeic acid
3.2.1.3
0.0582
-
pH not specified in the publication, 37C, recombinant glucoamylase subunit Ct-SI
Mus musculus
caffeic acid
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.3
(+)-catechin
noncompetitive inhibition
Mus musculus
3.2.1.3
caffeic acid
noncompetitive inhibition
Mus musculus
3.2.1.3
chlorogenic acid
noncompetitive inhibition
Mus musculus
3.2.1.3
epigallocatechin gallate
EGCG, noncompetitive inhibition
Mus musculus
3.2.1.3
gallic acid
noncompetitive inhibition
Mus musculus
3.2.1.3
additional information
kinetics and mechanism of inhibition of the recombinant glucoamylase subunit Ct-MGAM and sucrase subunit Ct-SI, overview
Mus musculus
3.2.1.48
(+)-catechin
noncompetitive
Homo sapiens
3.2.1.48
(+)-catechin
noncompetitive
Mus musculus
3.2.1.48
(-)-epigallocatechin gallate
noncompetitive
Homo sapiens
3.2.1.48
(-)-epigallocatechin gallate
noncompetitive
Mus musculus
3.2.1.48
caffeic acid
noncompetitive
Homo sapiens
3.2.1.48
caffeic acid
noncompetitive
Mus musculus
3.2.1.48
chlorogenic acid
noncompetitive
Homo sapiens
3.2.1.48
chlorogenic acid
noncompetitive
Mus musculus
3.2.1.48
gallic acid
noncompetitive
Homo sapiens
3.2.1.48
gallic acid
noncompetitive
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.3
membrane
-
Mus musculus
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.3
starch + H2O
Mus musculus
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.3
recombinant glucoamylase subunit Ct-MGAM splice form N20 and subunit Ct-SI from Spodoptera frugiperda Sf9 cells
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.3
starch + H2O
-
750886
Mus musculus
?
-
-
-
?
3.2.1.3
starch + H2O
soluble starch
750886
Mus musculus
?
-
-
-
?
3.2.1.48
maltose + H2O
-
750886
Homo sapiens
alpha-D-glucose
-
-
-
?
3.2.1.48
maltose + H2O
-
750886
Mus musculus
alpha-D-glucose
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.2.1.3
37
-
assay at
Mus musculus