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Literature summary extracted from

  • Zhu, Y.; Zhao, R.; Xiao, A.; Li, L.; Jiang, Z.; Chen, F.; Ni, H.
    Characterization of an alkaline beta-agarase from Stenotrophomonas sp. NTa and the enzymatic hydrolysates (2016), Int. J. Biol. Macromol., 86, 525-534 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B1 2-mercaptoethanol 103.1% activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 additional information not activated by dithiothreitol and phenylmethylsulfonyl fluoride Stenotrophomonas sp. NTa
3.2.1.B1 Triton X-100 106.7% activity at 1% (v/v) Stenotrophomonas sp. NTa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B1 Al3+ 56.3% residual activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Cd2+ 50.9% residual activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Co2+ 87.1% residual activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Cu2+ 15.8% residual activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 EDTA 68.1% residual activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Fe2+ 79.6% residual activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Fe3+ 4.4% residual activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Mn2+ 28.6% residual activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 SDS 72.8% residual activity at 1% (w/v) Stenotrophomonas sp. NTa
3.2.1.B1 Tween 20 95.7% residual activity at 1% (v/v) Stenotrophomonas sp. NTa
3.2.1.B1 Tween 80 97.5% residual activity at 1% (v/v) Stenotrophomonas sp. NTa
3.2.1.B1 Urea 84.8% residual activity at 5 M Stenotrophomonas sp. NTa
3.2.1.B1 Zn2+ 17.5% residual activity at 10 mM Stenotrophomonas sp. NTa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B1 extracellular
-
 Stenotrophomonas sp. NTa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.B1 Ag+ 103.6% activity at 1 mM Stenotrophomonas sp. NTa
3.2.1.B1 Ba2+ 153.5% activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Ca2+ 128.1% activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 K+ 146.4% activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Mg2+ 147.4% activity at 10 mM Stenotrophomonas sp. NTa
3.2.1.B1 Na+ 128.6% activity at 10 mM Stenotrophomonas sp. NTa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B1 179700
-
 gel filtration Stenotrophomonas sp. NTa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B1 Stenotrophomonas sp. NTa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B1 DEAE-Sepharose column chromatography and Sephacryl S-100HR gel filtration Stenotrophomonas sp. NTa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B1 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Stenotrophomonas sp. NTa 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.B1 agar + H2O
-
 Stenotrophomonas sp. NTa neoagarohexaose + neoagarotetraose + neoagarobiose + 3,6-anhydro-alpha-L-galactose
-
 ?
3.2.1.B1 additional information no activity with 4-nitrophenyl alpha-D-galactopyranoside Stenotrophomonas sp. NTa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B1 homodimer 2 * 89000, SDS-PAGE Stenotrophomonas sp. NTa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B1 beta-agarase
-
 Stenotrophomonas sp. NTa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B1 40
-
-
 Stenotrophomonas sp. NTa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B1 35 50 in temperatures ranging from 35 to 50°C, the enzyme presents over 40% of the maximum activity Stenotrophomonas sp. NTa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B1 20 60 the enzyme is relatively stable at 20°C and 30°C after incubation for 1 h. Incubation at higher temperatures results in enzyme activity loss. The enzyme retains 64% and 46% of residual activities after incubation at 40°C and 50°C for 1 h, individually. It has no activity at 60°C for 30 min Stenotrophomonas sp. NTa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B1 760.1
-
 agar at pH 10.0 and 40°C Stenotrophomonas sp. NTa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B1 10
-
-
 Stenotrophomonas sp. NTa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B1 7 11 the enzyme displays over 30% of its maximal activity in the pH range of 7.0-11.0. There is no detectable enzyme activity at pH 5.0. The enzyme still has 32% of relative activity at pH 11.0 Stenotrophomonas sp. NTa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.B1 5 11 the enzyme exhibits good stability in pH ranging from 5.0 to 11.0, retaining more than 65% of its original activity after 1 h incubation Stenotrophomonas sp. NTa