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Literature summary extracted from
- Biochemical features and kinetic properties of alpha-amylases from marine organisms (2016), Int. J. Biol. Macromol., 83, 306-314 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | recombinant expression of GST-tagged enzyme | Zunongwangia profunda |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ag+ | - |
Vibrio sp. |  |
| 3.2.1.1 | Al3+ | - |
Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | Al3+ | - |
Wangia sp. C52 | |
| 3.2.1.1 | cetyltrimethylammonium bromide | CTAB | Zunongwangia profunda | |
| 3.2.1.1 | Co2+ | - |
Streptomyces coelicolor A3 | |
| 3.2.1.1 | Co2+ | - |
Zunongwangia profunda | |
| 3.2.1.1 | Cu2+ | - |
Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | Cu2+ | - |
Streptomyces coelicolor A3 | |
| 3.2.1.1 | Cu2+ | - |
Vibrio sp. | |
| 3.2.1.1 | Cu2+ | - |
Wangia sp. C52 | |
| 3.2.1.1 | Cu2+ | - |
Zunongwangia profunda | |
| 3.2.1.1 | DMSO | - |
Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | EDTA | - |
Bacillus cereus | |
| 3.2.1.1 | EDTA | - |
Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | EDTA | - |
Rossellomorea aquimaris | |
| 3.2.1.1 | EDTA | - |
Streptomyces sp. D1 | |
| 3.2.1.1 | EDTA | - |
Vibrio sp. | |
| 3.2.1.1 | EDTA | - |
Wangia sp. C52 | |
| 3.2.1.1 | EDTA | - |
Zunongwangia profunda | |
| 3.2.1.1 | EGTA | - |
Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | EGTA | - |
Vibrio sp. | |
| 3.2.1.1 | EGTA | - |
Wangia sp. C52 | |
| 3.2.1.1 | Fe2+ | - |
Anoxybacillus beppuensis | |
| 3.2.1.1 | Fe3+ | - |
Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | Fe3+ | - |
Streptomyces sp. D1 | |
| 3.2.1.1 | Fe3+ | - |
Wangia sp. C52 | |
| 3.2.1.1 | Fe3+ | - |
Zunongwangia profunda | |
| 3.2.1.1 | Hg2+ | - |
Anoxybacillus flavithermus | |
| 3.2.1.1 | Hg2+ | - |
Bacillus cereus | |
| 3.2.1.1 | Hg2+ | - |
Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | Hg2+ | - |
Streptomyces coelicolor A3 | |
| 3.2.1.1 | Hg2+ | - |
Streptomyces sp. D1 | |
| 3.2.1.1 | Hg2+ | - |
Vibrio sp. | |
| 3.2.1.1 | Hg2+ | - |
Wangia sp. C52 | |
| 3.2.1.1 | Mg2+ | - |
Anoxybacillus beppuensis | |
| 3.2.1.1 | Mn2+ | - |
Wangia sp. C52 | |
| 3.2.1.1 | Mn2+ | - |
Zunongwangia profunda | |
| 3.2.1.1 | additional information | the enzyme is resistant to SDS | Bacillus sp. TS-23 | |
| 3.2.1.1 | Na+ | - |
Anoxybacillus beppuensis | |
| 3.2.1.1 | NaCl | inhibits over 80% | Nocardiopsis sp. B2 | |
| 3.2.1.1 | Ni2+ | - |
Zunongwangia profunda | |
| 3.2.1.1 | Pb2+ | - |
Bacillus cereus | |
| 3.2.1.1 | SDS | - |
Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | SDS | - |
Wangia sp. C52 | |
| 3.2.1.1 | SDS | - |
Zunongwangia profunda | |
| 3.2.1.1 | Urea | - |
Streptomyces coelicolor A3 | |
| 3.2.1.1 | Zn2+ | - |
Wangia sp. C52 | |
| 3.2.1.1 | Zn2+ | - |
Zunongwangia profunda | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
additional information | Km is 0.5 mg/ml and Vmax is 3.571 mmol/ml/mg | Anoxybacillus beppuensis | |
| 3.2.1.1 | additional information | - |
additional information | Km is 1.7 mg/ml and Vmax is 0.5 mg/ml/min | Vibrio sp. | |
| 3.2.1.1 | additional information | - |
additional information | Km is 1.73 mg/ml and Vmax is 1.24 mg/ml/min | Pseudomonas sp. K6-28-040 | |
| 3.2.1.1 | additional information | - |
additional information | Km is 11.71 mg/ml and Vmax is 0.14 mg/ml/min | Zunongwangia profunda | |
| 3.2.1.1 | additional information | - |
additional information | Km is 2.08 mg/ml and Vmax is 1.26 mg/ml/min | Wangia sp. C52 | |
| 3.2.1.1 | additional information | - |
additional information | Km is 4.79 mg/ml and Vmax is 0.322 mM/min | Bacillus sp. ALSHL3 | |
| 3.2.1.1 | additional information | - |
additional information | Km is 5.8 mg/ml and Vmax is 0.25 mg/ml/min | Aureobasidium pullulans | |
| 3.2.1.1 | 0.005 | - |
starch | pH 7.0, 55°C | Anoxybacillus flavithermus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Mn2+ | activates | Bacillus cereus | |
| 3.2.1.1 | additional information | calcium ions show neither inhibitory nor potentiating activity on alpha-amylase activity, alpha-amylase extracted from marine Streptomyces strain A3 is mainly calcium independent | Streptomyces coelicolor A3 | |
| 3.2.1.1 | NaCl | the enzyme is resistant against 4.5% NaCl | Halobacillus sp. amylus | |
| 3.2.1.1 | NaCl | the enzyme is resistant to 11% NaCl | Bacillus sp. TSCVKK | |
| 3.2.1.1 | NaCl | the enzyme is resistant to 4.5% NaCl | Bacillus sp. marini | |
| 3.2.1.1 | NaCl | the enzyme is stable against 0.5-4.0 M, a salt-tolerant alpha-amylase | Zunongwangia profunda | |
| 3.2.1.1 | NaCl | the enzyme is stable against 1.5 M NaCl | Bacillus sp. ALSHL3 | |
| 3.2.1.1 | NaCl | the enzyme is stable against 10-17% | Streptomyces coelicolor A3 | |
| 3.2.1.1 | NaCl | the enzyme is stable against 11-17% | Saccharopolyspora sp. A9 | |
| 3.2.1.1 | NaCl | the enzyme is stable against 12% | Streptomyces sp. D1 | |
| 3.2.1.1 | NaCl | the enzyme is stable against 3.5 M NaCl | Rossellomorea aquimaris | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Anoxybacillus beppuensis | - |
- |
- |
| 3.2.1.1 | Anoxybacillus beppuensis TSSC-1 | - |
- |
- |
| 3.2.1.1 | Anoxybacillus flavithermus | - |
- |
- |
| 3.2.1.1 | Aureobasidium pullulans | - |
- |
- |
| 3.2.1.1 | Aureobasidium pullulans N13d | - |
- |
- |
| 3.2.1.1 | Bacillus cereus | - |
- |
- |
| 3.2.1.1 | Bacillus licheniformis | A1YR25 | - |
- |
| 3.2.1.1 | Bacillus licheniformis NH1 | A1YR25 | - |
- |
| 3.2.1.1 | Bacillus sp. A-3-15 | - |
- |
- |
| 3.2.1.1 | Bacillus sp. ALSHL3 | - |
- |
- |
| 3.2.1.1 | Bacillus sp. Ferdowsicous | P86331 | - |
- |
| 3.2.1.1 | Bacillus sp. GM8901 | - |
- |
- |
| 3.2.1.1 | Bacillus sp. I-3 | - |
- |
- |
| 3.2.1.1 | Bacillus sp. KSM-K38 | Q93I48 | - |
- |
| 3.2.1.1 | Bacillus sp. marini | - |
- |
- |
| 3.2.1.1 | Bacillus sp. TS-23 | - |
- |
- |
| 3.2.1.1 | Bacillus sp. TSCVKK | - |
- |
- |
| 3.2.1.1 | Bacillus sp. WN 11 | - |
- |
- |
| 3.2.1.1 | Bacillus sp. YX-1 | A9YDD9 | - |
- |
| 3.2.1.1 | Bacillus subtilis | - |
- |
- |
| 3.2.1.1 | Bacillus subtilis AX20 | - |
- |
- |
| 3.2.1.1 | Bacillus subtilis DM-03 | - |
- |
- |
| 3.2.1.1 | Chromohalobacter sp. TVSP 101 | - |
- |
- |
| 3.2.1.1 | Chryseobacterium taeanense | A0A1G8DI07 | - |
- |
| 3.2.1.1 | Chryseobacterium taeanense TKU001 | A0A1G8DI07 | - |
- |
| 3.2.1.1 | Eisenia fetida | - |
- |
- |
| 3.2.1.1 | Halobacillus sp. amylus | - |
- |
- |
| 3.2.1.1 | Halobacillus sp. amylus HM454199 | - |
- |
- |
| 3.2.1.1 | Marinobacter sp. EMB8 | - |
- |
- |
| 3.2.1.1 | Nesterenkonia sp. | A0A2U8ZSD8 | - |
- |
| 3.2.1.1 | Nesterenkonia sp. F | A0A2U8ZSD8 | - |
- |
| 3.2.1.1 | Nocardiopsis sp. B2 | - |
- |
- |
| 3.2.1.1 | Parageobacillus caldoxylosilyticus | C0LZ63 | i.e. Geobacillus caldoxylosilyticus | - |
| 3.2.1.1 | Parageobacillus caldoxylosilyticus TK4 | C0LZ63 | i.e. Geobacillus caldoxylosilyticus | - |
| 3.2.1.1 | Pseudomonas sp. K6-28-040 | - |
- |
- |
| 3.2.1.1 | Rossellomorea aquimaris | - |
- |
- |
| 3.2.1.1 | Rossellomorea aquimaris VITP4 | - |
- |
- |
| 3.2.1.1 | Saccharopolyspora sp. A9 | - |
- |
- |
| 3.2.1.1 | Streptomyces coelicolor A3 | Q9L035 | - |
- |
| 3.2.1.1 | Streptomyces coelicolor A3 A3(2) | Q9L035 | - |
- |
| 3.2.1.1 | Streptomyces coelicolor A3 ATCC BAA-471 | Q9L035 | - |
- |
| 3.2.1.1 | Streptomyces coelicolor A3 M145 | Q9L035 | - |
- |
| 3.2.1.1 | Streptomyces sp. D1 | - |
- |
- |
| 3.2.1.1 | Sutcliffiella cohnii | A0A223KY92 | - |
- |
| 3.2.1.1 | Sutcliffiella cohnii US147 | A0A223KY92 | - |
- |
| 3.2.1.1 | Thermomonospora curvata | - |
- |
- |
| 3.2.1.1 | Thermus sp. | - |
- |
- |
| 3.2.1.1 | Vibrio sp. | - |
- |
- |
| 3.2.1.1 | Wangia sp. C52 | - |
- |
- |
| 3.2.1.1 | Zunongwangia profunda | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | glycoprotein | - |
Vibrio sp. |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | 163fold by starch affinity chromatography | Vibrio sp. |
| 3.2.1.1 | 16fold by ammonium sulfate fractionation and anion exchange chromatography | Bacillus cereus |
| 3.2.1.1 | 20fold by ammonium sulfate fractionation and hydrophobic interaction chromatography | Anoxybacillus beppuensis |
| 3.2.1.1 | 24fold by ammonium sulfate fractionation and gel filtration | Wangia sp. C52 |
| 3.2.1.1 | 39fold by ammonium sulfate fractionation and gel filtration | Saccharopolyspora sp. A9 |
| 3.2.1.1 | 44fold by gel filtration | Streptomyces coelicolor A3 |
| 3.2.1.1 | 44fold by gel filtration | Nocardiopsis sp. B2 |
| 3.2.1.1 | 5fold by ammonium sulfate fractionation and gel filtration | Pseudomonas sp. K6-28-040 |
| 3.2.1.1 | GST-tagged enzyme 333fold by glutathione affinity chromatography | Zunongwangia profunda |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | additional information | grows on maltose | Streptomyces coelicolor A3 | - |
| 3.2.1.1 | additional information | grows on soluble starch | Bacillus cereus | - |
| 3.2.1.1 | additional information | grows on starch | Anoxybacillus flavithermus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 44 | - |
purified enzyme, pH and temperature not specified in the publication | Bacillus cereus |
| 3.2.1.1 | 135 | - |
purified enzyme, pH and temperature not specified in the publication | Pseudomonas sp. K6-28-040 |
| 3.2.1.1 | 146 | - |
purified enzyme, pH and temperature not specified in the publication | Wangia sp. C52 |
| 3.2.1.1 | 327 | - |
purified enzyme, pH 6.5, 60°C | Vibrio sp. |
| 3.2.1.1 | 1633 | - |
purified enzyme, pH and temperature not specified in the publication | Zunongwangia profunda |
| 3.2.1.1 | 1641 | - |
purified enzyme, pH and temperature not specified in the publication | Saccharopolyspora sp. A9 |
| 3.2.1.1 | 1755 | - |
purified enzyme, pH and temperature not specified in the publication | Streptomyces coelicolor A3 |
| 3.2.1.1 | 1755 | - |
purified enzyme, pH and temperature not specified in the publication | Nocardiopsis sp. B2 |
| 3.2.1.1 | 10000 | - |
purified enzyme, pH and temperature not specified in the publication | Anoxybacillus beppuensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | corn starch + H2O | - |
Vibrio sp. | ? | - |
? | |
| 3.2.1.1 | corn starch + H2O | - |
Nocardiopsis sp. B2 | ? | - |
? | |
| 3.2.1.1 | additional information | a saccharifying enzyme | Bacillus sp. ALSHL3 | ? | - |
? | |
| 3.2.1.1 | soluble starch + H2O | - |
Bacillus cereus | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus subtilis | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Thermus sp. | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Anoxybacillus flavithermus | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Thermomonospora curvata | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Eisenia fetida | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Parageobacillus caldoxylosilyticus | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Streptomyces coelicolor A3 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. KSM-K38 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. YX-1 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Sutcliffiella cohnii | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Nesterenkonia sp. | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Chryseobacterium taeanense | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Marinobacter sp. EMB8 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. Ferdowsicous | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Saccharopolyspora sp. A9 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Wangia sp. C52 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Pseudomonas sp. K6-28-040 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Anoxybacillus beppuensis | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Zunongwangia profunda | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Rossellomorea aquimaris | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. ALSHL3 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Streptomyces sp. D1 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. GM8901 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. TS-23 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. I-3 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. TSCVKK | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. WN 11 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. A-3-15 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Chromohalobacter sp. TVSP 101 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. marini | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Halobacillus sp. amylus | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Aureobasidium pullulans N13d | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus subtilis AX20 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Chryseobacterium taeanense TKU001 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus subtilis DM-03 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Anoxybacillus beppuensis TSSC-1 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Sutcliffiella cohnii US147 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Rossellomorea aquimaris VITP4 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Halobacillus sp. amylus HM454199 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Parageobacillus caldoxylosilyticus TK4 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Nesterenkonia sp. F | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Streptomyces coelicolor A3 A3(2) | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Bacillus licheniformis NH1 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Streptomyces coelicolor A3 M145 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | - |
Streptomyces coelicolor A3 ATCC BAA-471 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ? | x * 60000, about, SDS-PAGE | Wangia sp. C52 |
| 3.2.1.1 | ? | x * 60000, about, SDS-PAGE | Pseudomonas sp. K6-28-040 |
| 3.2.1.1 | ? | x * 70000, about, SDS-PAGE | Saccharopolyspora sp. A9 |
| 3.2.1.1 | ? | x * 70000, about, SDS-PAGE | Streptomyces sp. D1 |
| 3.2.1.1 | ? | x * 62000, about, SDS-PAGE | Thermomonospora curvata |
| 3.2.1.1 | ? | x * 80000, about, SDS-PAGE | Bacillus subtilis |
| 3.2.1.1 | ? | x * 55000, about, SDS-PAGE | Bacillus cereus |
| 3.2.1.1 | ? | x * 55000, about, SDS-PAGE | Vibrio sp. |
| 3.2.1.1 | ? | x * 55000, about, SDS-PAGE | Eisenia fetida |
| 3.2.1.1 | ? | x * 55000, about, SDS-PAGE | Bacillus licheniformis |
| 3.2.1.1 | ? | x * 55000, about, SDS-PAGE | Bacillus sp. I-3 |
| 3.2.1.1 | ? | x * 43000, about, SDS-PAGE | Bacillus subtilis |
| 3.2.1.1 | ? | x * 45000, about, SDS-PAGE | Nocardiopsis sp. B2 |
| 3.2.1.1 | ? | x * 58000, about, SDS-PAGE | Thermus sp. |
| 3.2.1.1 | ? | x * 58000, about, SDS-PAGE | Bacillus sp. KSM-K38 |
| 3.2.1.1 | ? | x * 58000, about, SDS-PAGE | Bacillus sp. YX-1 |
| 3.2.1.1 | ? | x * 65000, about, SDS-PAGE | Anoxybacillus flavithermus |
| 3.2.1.1 | ? | x * 68000, about, SDS-PAGE | Bacillus sp. TS-23 |
| 3.2.1.1 | ? | x * 75000, about, SDS-PAGE | Bacillus sp. ALSHL3 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | AmyE | - |
Bacillus cereus |
| 3.2.1.1 | AmyK38 | - |
Bacillus sp. KSM-K38 |
| 3.2.1.1 | AmyZ2 | - |
Zunongwangia profunda |
| 3.2.1.1 | B4168_3135 | - |
Anoxybacillus flavithermus |
| 3.2.1.1 | cold-adapted alpha-amylase | - |
Wangia sp. C52 |
| 3.2.1.1 | haloalkaline alpha-amylase | - |
Zunongwangia profunda |
| 3.2.1.1 | maltogenic amylase | UniProt | Parageobacillus caldoxylosilyticus |
| 3.2.1.1 | salt-tolerant alpha-amylase | - |
Zunongwangia profunda |
| 3.2.1.1 | salt-tolerant alpha-amylase | - |
Bacillus sp. ALSHL3 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 30 | - |
- |
Wangia sp. C52 |
| 3.2.1.1 | 40 | - |
- |
Rossellomorea aquimaris |
| 3.2.1.1 | 40 | - |
- |
Bacillus sp. marini |
| 3.2.1.1 | 40 | - |
- |
Halobacillus sp. amylus |
| 3.2.1.1 | 40 | 50 | - |
Bacillus sp. YX-1 |
| 3.2.1.1 | 45 | - |
- |
Nesterenkonia sp. |
| 3.2.1.1 | 45 | - |
- |
Nocardiopsis sp. B2 |
| 3.2.1.1 | 45 | - |
- |
Streptomyces sp. D1 |
| 3.2.1.1 | 50 | - |
- |
Thermus sp. |
| 3.2.1.1 | 50 | - |
- |
Eisenia fetida |
| 3.2.1.1 | 50 | - |
- |
Pseudomonas sp. K6-28-040 |
| 3.2.1.1 | 50 | - |
- |
Zunongwangia profunda |
| 3.2.1.1 | 55 | - |
- |
Bacillus subtilis |
| 3.2.1.1 | 55 | - |
- |
Anoxybacillus flavithermus |
| 3.2.1.1 | 55 | - |
- |
Saccharopolyspora sp. A9 |
| 3.2.1.1 | 55 | 60 | - |
Bacillus sp. KSM-K38 |
| 3.2.1.1 | 60 | - |
- |
Vibrio sp. |
| 3.2.1.1 | 60 | - |
- |
Bacillus sp. ALSHL3 |
| 3.2.1.1 | 60 | - |
- |
Bacillus sp. GM8901 |
| 3.2.1.1 | 60 | - |
- |
Bacillus sp. TS-23 |
| 3.2.1.1 | 65 | - |
- |
Bacillus cereus |
| 3.2.1.1 | 65 | - |
- |
Thermomonospora curvata |
| 3.2.1.1 | 70 | - |
- |
Sutcliffiella cohnii |
| 3.2.1.1 | 70 | - |
- |
Bacillus sp. Ferdowsicous |
| 3.2.1.1 | 70 | - |
- |
Bacillus sp. I-3 |
| 3.2.1.1 | 70 | - |
- |
Bacillus sp. A-3-15 |
| 3.2.1.1 | 75 | 80 | - |
Bacillus sp. WN 11 |
| 3.2.1.1 | 80 | - |
- |
Anoxybacillus beppuensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
reversible unfolding even at high temperature | Rossellomorea aquimaris |
| 3.2.1.1 | additional information | - |
the purfied enzyme is moderately thermostable | Streptomyces coelicolor A3 |
| 3.2.1.1 | 37 | 100 | purified enzyme, stable at | Anoxybacillus beppuensis |
| 3.2.1.1 | 40 | 45 | purified enzyme, stable at | Anoxybacillus flavithermus |
| 3.2.1.1 | 40 | 60 | purified enzyme, stable at | Zunongwangia profunda |
| 3.2.1.1 | 50 | - |
purified enzyme, stable up to | Vibrio sp. |
| 3.2.1.1 | 60 | - |
stable up to | Bacillus subtilis |
| 3.2.1.1 | 60 | - |
stable up to | Thermus sp. |
| 3.2.1.1 | 65 | - |
purified enzyme, stable up to | Bacillus cereus |
| 3.2.1.1 | 70 | - |
stable up to | Bacillus sp. ALSHL3 |
| 3.2.1.1 | 75 | 85 | purified enzyme, stable at | Nocardiopsis sp. B2 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | 1449.43 | - |
starch | pH and temperature not specified in the publication | Zunongwangia profunda | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 4.5 | - |
- |
Bacillus sp. Ferdowsicous |
| 3.2.1.1 | 5 | - |
- |
Bacillus sp. YX-1 |
| 3.2.1.1 | 5.5 | - |
- |
Eisenia fetida |
| 3.2.1.1 | 5.5 | - |
- |
Bacillus sp. WN 11 |
| 3.2.1.1 | 5.5 | 6.5 | - |
Thermus sp. |
| 3.2.1.1 | 6 | - |
- |
Bacillus subtilis |
| 3.2.1.1 | 6 | - |
- |
Wangia sp. C52 |
| 3.2.1.1 | 6 | - |
- |
Bacillus sp. ALSHL3 |
| 3.2.1.1 | 6.5 | - |
- |
Vibrio sp. |
| 3.2.1.1 | 6.5 | - |
- |
Bacillus licheniformis |
| 3.2.1.1 | 7 | - |
- |
Anoxybacillus flavithermus |
| 3.2.1.1 | 7 | - |
- |
Parageobacillus caldoxylosilyticus |
| 3.2.1.1 | 7 | - |
- |
Marinobacter sp. EMB8 |
| 3.2.1.1 | 7 | - |
- |
Pseudomonas sp. K6-28-040 |
| 3.2.1.1 | 7 | - |
- |
Anoxybacillus beppuensis |
| 3.2.1.1 | 7 | - |
- |
Zunongwangia profunda |
| 3.2.1.1 | 7 | - |
- |
Bacillus sp. I-3 |
| 3.2.1.1 | 7 | - |
- |
Bacillus sp. marini |
| 3.2.1.1 | 7.5 | - |
- |
Nesterenkonia sp. |
| 3.2.1.1 | 7.5 | 9.5 | - |
Rossellomorea aquimaris |
| 3.2.1.1 | 8 | - |
- |
Halobacillus sp. amylus |
| 3.2.1.1 | 8 | 9 | - |
Bacillus sp. KSM-K38 |
| 3.2.1.1 | 8 | 11 | - |
Bacillus cereus |
| 3.2.1.1 | 9 | - |
- |
Bacillus subtilis |
| 3.2.1.1 | 9 | - |
- |
Sutcliffiella cohnii |
| 3.2.1.1 | 9 | - |
- |
Chryseobacterium taeanense |
| 3.2.1.1 | 9 | - |
- |
Nocardiopsis sp. B2 |
| 3.2.1.1 | 9 | - |
- |
Streptomyces sp. D1 |
| 3.2.1.1 | 9 | - |
- |
Bacillus sp. TS-23 |
| 3.2.1.1 | 9 | - |
- |
Chromohalobacter sp. TVSP 101 |
| 3.2.1.1 | 11 | - |
- |
Saccharopolyspora sp. A9 |
| 3.2.1.1 | 11 | - |
- |
Bacillus sp. TSCVKK |
| 3.2.1.1 | 11 | - |
- |
Bacillus sp. A-3-15 |
| 3.2.1.1 | 11 | 12 | - |
Bacillus sp. GM8901 |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 3 | 9 | stable at | Parageobacillus caldoxylosilyticus |
| 3.2.1.1 | 4 | 9 | stable at | Bacillus licheniformis |
| 3.2.1.1 | 4.5 | 11 | purified enzyme, stable at | Bacillus sp. YX-1 |
| 3.2.1.1 | 5 | 9 | stable at | Bacillus subtilis |
| 3.2.1.1 | 5 | 10 | purified enzyme, stable at | Pseudomonas sp. K6-28-040 |
| 3.2.1.1 | 5 | 10 | purified enzyme, stable at | Anoxybacillus beppuensis |
| 3.2.1.1 | 5.5 | 9 | purified enzyme, stable at | Bacillus sp. WN 11 |
| 3.2.1.1 | 6 | 10 | purified enzyme, stable at | Zunongwangia profunda |
| 3.2.1.1 | 6 | - |
purified enzyme, stable at | Thermomonospora curvata |
| 3.2.1.1 | 6 | 11 | stable at | Bacillus sp. KSM-K38 |
| 3.2.1.1 | 6 | 11 | stable at | Marinobacter sp. EMB8 |
| 3.2.1.1 | 6 | 13 | stable at | Bacillus sp. GM8901 |
| 3.2.1.1 | 6.5 | 7.5 | purified enzyme, stable at | Vibrio sp. |
| 3.2.1.1 | 6.5 | 7.5 | purified enzyme, stable at | Rossellomorea aquimaris |
| 3.2.1.1 | 7 | - |
purified enzyme, stable at | Anoxybacillus flavithermus |
| 3.2.1.1 | 7 | 11 | stable at | Streptomyces sp. D1 |
| 3.2.1.1 | 7 | 11 | purified enzyme, stable at | Nocardiopsis sp. B2 |
| 3.2.1.1 | 7 | 10 | stable at | Chromohalobacter sp. TVSP 101 |
| 3.2.1.1 | 7 | 10 | purified enzyme, stable at | Bacillus sp. TS-23 |
| 3.2.1.1 | 8 | 12 | purified enzyme, stable at | Saccharopolyspora sp. A9 |
| 3.2.1.1 | 9 | - |
stable up to | Sutcliffiella cohnii |
| 3.2.1.1 | 9 | - |
purified enzyme, stable at | Streptomyces coelicolor A3 |
| 3.2.1.1 | 9 | 11 | stable at | Chryseobacterium taeanense |
| 3.2.1.1 | 10 | 12 | stable at | Bacillus sp. A-3-15 |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.1 | Wangia sp. C52 | - |
- |
5.6 |
| 3.2.1.1 | Vibrio sp. | - |
- |
6.2 |
| 3.2.1.1 | Pseudomonas sp. K6-28-040 | - |
7.8 | 7.5 |
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Release 2023.1 (January 2023)
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