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Literature summary extracted from

  • Laribi-Habchi, H.; Bouanane-Darenfed, A.; Drouiche, N.; Pauss, A.; Mameri, N.
    Purification, characterization, and molecular cloning of an extracellular chitinase from Bacillus licheniformis stain LHH100 isolated from wastewater samples in Algeria (2015), Int. J. Biol. Macromol., 72, 1117-1128 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.200 Cd2+ 5 mM, 35% residual activity Bacillus licheniformis
3.2.1.200 dithiothreitol 2 mM, 10% residual activity Bacillus licheniformis
3.2.1.200 Hg2+ 5 mM, complete inhibition Bacillus licheniformis
3.2.1.200 N-ethylmaleimide 2 mM, complete inhibition Bacillus licheniformis
3.2.1.200 p-chloromercuribenzoate 2 mM, complete inhibition Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.200 additional information
-
 colloidal chitin Km value 0.385 mg/ml, pH 4.0, 70°C Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.200 Al3+ 5 mM, 113% of initial activity Bacillus licheniformis
3.2.1.200 K+ 5 mM, 145% of initial activity Bacillus licheniformis
3.2.1.200 Li+ 5 mM, 132% of initial activity Bacillus licheniformis
3.2.1.200 Zn2+ 5 mM, 131% of initial activity Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.200 65193
-
 MALDI-TOF Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.200 Bacillus licheniformis A0A0G2QUN1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.200 4-nitrophenyl beta-N,N',N'', N'''-tetraacetylchitotetraoside + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.200 4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.200 4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.200 chitooligosaccharide + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.200 chitosan + H2O
-
 Bacillus licheniformis N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.200 chitotriose + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.200 colloidal chitin + H2O
-
 Bacillus licheniformis N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.200 glycol chitin + H2O
-
 Bacillus licheniformis N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.200 additional information the enzmye preferentially hydrolyzes the second glycosidic link from the non-reducing end of (GlcNAc)n Bacillus licheniformis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.200 monomer 1 * 65193, MALDI-TOF Bacillus licheniformis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.200 ChiA 65
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.200 75
-
-
 Bacillus licheniformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.200 5000
-
 colloidal chitin pH 4.0, 70°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.200 4
-
-
 Bacillus licheniformis