Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ali, B.; Yi, Z.; Fang, Y.; Chen, L.; He, K.; Liu, D.; Luo, H.; Zhao, D.; Zheng, J.; He, H.; Jin, Y.; Zhao, H.
    Characterization of a fungal thermostable endoglucanase from Chinese Nong-flavor daqu by metatranscriptomic method (2019), Int. J. Biol. Macromol., 121, 183-190 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 gene NFEg16A, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as intracellular soluble protein uncultured Paecilomyces

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 Al3+ 35% inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 Ba2+ 18% inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 Co2+ 43% inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 Cu2+ 35% inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 Fe3+ complete inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 Hg2+ complete inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 K+ 15% inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 Li+ 8.5% inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 Mg2+ 12% inhibition at 10 mM uncultured Paecilomyces
3.2.1.73 SDS 14% inhibition at 10 mM uncultured Paecilomyces

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.73 extracellular enzyme NFEg16A has a signal peptide uncultured Paecilomyces
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.73 additional information no or poor effects by NaCl, NH4Cl, NiCl2, ZnSO4 and EDTA uncultured Paecilomyces

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 uncultured Paecilomyces A0A386YJ14 uncultured bacterium from Chinese Nong-flavor daqu
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration uncultured Paecilomyces

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 85.3
-
 purified recombinant enzyme, pH 6.5, 60°C, substrate barley beta-glucan uncultured Paecilomyces

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O enzyme NFEg16A efficiently hydrolyzes the substrate at a high concentration of 15 mg/mL, and releases glucose and cellobiose as its main end products uncultured Paecilomyces beta-D-glucose + cellobiose + ?
-
 ?
3.2.1.73 additional information enzyme NFEg16A does not show clear activity against nature product of laminarin and carboxymethylcellulose-Na, but shows activity on barley beta-glucan, which might indicate that it is a true beta-1,3-1,4-glucanase (EC 3.2.1.73) uncultured Paecilomyces ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 33300, recombinant His-tagged enzyme, SDS-PAGE uncultured Paecilomyces
3.2.1.73 More the enzyme has an endo-1,3-1,4-beta-glucanase domain uncultured Paecilomyces

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 endo-beta-glucanase
-
 uncultured Paecilomyces
3.2.1.73 endotype beta-1,3-1,4-glucanase
-
 uncultured Paecilomyces
3.2.1.73 NFEg16A
-
 uncultured Paecilomyces

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 60
-
 recombinant enzyme uncultured Paecilomyces

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 30 85 over 80% of maximal activity within this range uncultured Paecilomyces

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 70
-
 purified recombinant enzyme, completely stable for 8 h, and loss of 20% activity after 22 h uncultured Paecilomyces
3.2.1.73 80 90 purified recombinant enzyme, 70% and 40% activity remaining after 2 h at 80°C and 90°C, respectively, half-lives of the enzyme are 3.4 h and 1.4 h at 80°C and 90°C, respectively uncultured Paecilomyces

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 6.5
-
 recombinant enzyme uncultured Paecilomyces

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 3 8 over 60% of maximal activity within this range uncultured Paecilomyces

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 3.5 8 purified recombinant enzyme, 30 min, over 80% activity remaining uncultured Paecilomyces

General Information

EC Number General Information Comment Organism
3.2.1.73 additional information the enzyme has an endo-1,3-1,4-beta-glucanase domain uncultured Paecilomyces