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Literature summary extracted from
- Bi-functional fusion enzyme (EG-M-Xyn)EG-M-Xyn displaying endoglucanase and xylanase activities and its utility in improving lignocellulose degradation (2018), Int. J. Biol. Macromol., 111, 722-729 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | acetone | 5%, 4.3fold activation of activity with carboxymethyl cellulose, slight activation of xylanase activity (to 104.6% of control), fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma |  |
| 3.2.1.4 | butanol | 5%, 1.1fold activation of activity with carboxymethyl cellulose, 73% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | EDTA | 5 mM, 90.6% inhibition of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | glycerol | 5%, 8.2fold activation of activity with carboxymethyl cellulose, 10% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | SDS | 1%, 5.7fold activation of activity with carboxymethyl cellulose, 4.5fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.8 | acetone | 5%, 4.3fold activation of activity with carboxymethyl cellulose, slight activation of xylanase activity (to 104.6% of control), fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | butanol | 5%, 1.1fold activation of activity with carboxymethyl cellulose, 73% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | EDTA | 5 mM, 90.6% inhibition of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | glycerol | 5%, 8.2fold activation of activity with carboxymethyl cellulose, 10% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | SDS | 1%, 5.7fold activation of activity with carboxymethyl cellulose, 4.5fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | biofuel production | the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus has great potential in generating fermentable sugars from renewable agro-residues for biofuel and fine chemical industry. Application of the fusion enzyme (EG-M-Xyn)in combination with Ctec2 (commercial enzyme) in the saccharification leads to a 10-20% net increase in fermentable sugars liberated from pretreated rice straw in comparison to the Ctec2 alone | Teleogryllus emma |
| 3.2.1.4 | industry | the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus has great potential in generating fermentable sugars from renewable agro-residues for biofuel and fine chemical industry. Application of the fusion enzyme (EG-M-Xyn)in combination with Ctec2 (commercial enzyme) in the saccharification leads to a 10-20% net increase in fermentable sugars liberated from pretreated rice straw in comparison to the Ctec2 alone | Teleogryllus emma |
| 3.2.1.8 | biofuel production | the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus has great potential in generating fermentable sugars from renewable agro-residues for biofuel and fine chemical industry. Application of the fusion enzyme (EG-M-Xyn)in combination with Ctec2 (commercial enzyme) in the saccharification leads to a 10-20% net increase in fermentable sugars liberated from pretreated rice straw in comparison to the Ctec2 alone | Thermomyces lanuginosus |
| 3.2.1.8 | industry | the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus has great potential in generating fermentable sugars from renewable agro-residues for biofuel and fine chemical industry. Application of the fusion enzyme (EG-M-Xyn)in combination with Ctec2 (commercial enzyme) in the saccharification leads to a 10-20% net increase in fermentable sugars liberated from pretreated rice straw in comparison to the Ctec2 alone | Thermomyces lanuginosus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | expression of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus in Yarrowialipolytica | Teleogryllus emma |
| 3.2.1.8 | expression of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus in Yarrowia lipolytica | Thermomyces lanuginosus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | additional information | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma |
| 3.2.1.8 | additional information | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | Cu2+ | 5 mM, 1.6fold activation of activity with carboxymethyl cellulose, 16.4% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | EDTA | 5 mM, 90.6% inhibition of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | ethanol | 5%, 16% inhibition of activity with carboxymethyl cellulose, 24% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Fe2+ | 5 mM, 2.9fold activation of activity with carboxymethyl cellulose, 6.7% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Fe3+ | 5 mM, 1.1fold activation of activity with carboxymethyl cellulose, 9.1% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Isopropanol | 5%, 21.6% inhibition of activity with carboxymethyl cellulose, 53% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | K+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 13% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | methanol | 5%, 26.5% inhibition of activity with carboxymethyl cellulose, 13.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Mg2+ | 5 mM, 2.3fold activation of activity with carboxymethyl cellulose, 6.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Na+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 6% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | NaN3 | 0.1%, 45.2% inhibition of activity with carboxymethyl cellulose, 12.25% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.8 | Cu2+ | 5 mM, 1.6fold activation of activity with carboxymethyl cellulose, 16.4% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | EDTA | 5 mM, 90.6% inhibition of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | ethanol | 5%, 16% inhibition of activity with carboxymethyl cellulose, 24% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Fe2+ | 5 mM, 2.9fold activation of activity with carboxymethyl cellulose, 6.7% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Fe3+ | 5 mM, 1.1fold activation of activity with carboxymethyl cellulose, 9.1% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Isopropanol | 5%, 21.6% inhibition of activity with carboxymethyl cellulose, 53% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | K+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 13% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | methanol | 5%, 26.5% inhibition of activity with carboxymethyl cellulose, 13.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Mg2+ | 5 mM, 2.3fold activation of activity with carboxymethyl cellulose, 6.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Na+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 6% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | NaN3 | 0.1%, 45.2% inhibition of activity with carboxymethyl cellulose, 12.25% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.4 | additional information | - |
carboxymethyl cellulose | Km-value: 6.8 mg/ml, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, pH 5.0, 70°C | Teleogryllus emma | |
| 3.2.1.4 | additional information | - |
beechwood xylan | Km-value: 60.6 mg/ml, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, pH 5.0, 70°C | Teleogryllus emma | |
| 3.2.1.8 | additional information | - |
carboxymethyl cellulose | Km-value: 6.8 mg/ml, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, pH 5.0, 70°C | Thermomyces lanuginosus | |
| 3.2.1.8 | additional information | - |
beechwood xylan | Km-value: 60.6 mg/ml, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, pH 5.0, 70°C | Thermomyces lanuginosus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | Ca2+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Co2+ | 5 mM, 3.3fold activation of activity with carboxymethyl cellulose, 1.4fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Cu2+ | 5 mM, 1.6fold activation of activity with carboxymethyl cellulose, 16.4% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Fe2+ | 5 mM, 2.9fold activation of activity with carboxymethyl cellulose, 6.7% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Fe3+ | 5 mM, 1.1fold activation of activity with carboxymethyl cellulose, 9.1% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | K+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 13% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Mg2+ | 5 mM, 2.3fold activation of activity with carboxymethyl cellulose, 6.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Mn2+ | 5 mM, 6.6fold activation of activity with carboxymethyl cellulose, 1.6fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Na+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 6% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.4 | Zn2+ | 5 mM, 1.7fold activation of activity with carboxymethyl cellulose, no effect on xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | |
| 3.2.1.8 | Ca2+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 1.1fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Co2+ | 5 mM, 3.3fold activation of activity with carboxymethyl cellulose, 1.4fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Cu2+ | 5 mM, 1.6fold activation of activity with carboxymethyl cellulose, 16.4% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Fe2+ | 5 mM, 2.9fold activation of activity with carboxymethyl cellulose, 6.7% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Fe3+ | 5 mM, 1.1fold activation of activity with carboxymethyl cellulose, 9.1% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | K+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 13% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Mg2+ | 5 mM, 2.3fold activation of activity with carboxymethyl cellulose, 6.5% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Mn2+ | 5 mM, 6.6fold activation of activity with carboxymethyl cellulose, 1.6fold activation of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Na+ | 5 mM, 1.3fold activation of activity with carboxymethyl cellulose, 6% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
| 3.2.1.8 | Zn2+ | 5 mM, 1.7fold activation of activity with carboxymethyl cellulose, no effect on xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 75500 | - |
gel filtration, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma |
| 3.2.1.8 | 75500 | - |
gel filtration, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Teleogryllus emma | A8CYP6 | - |
- |
| 3.2.1.8 | Thermomyces lanuginosus | O43097 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma |
| 3.2.1.8 | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | avicel + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | ? | - |
? | |
| 3.2.1.4 | barley glucan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | ? | - |
? | |
| 3.2.1.4 | beechwood xylan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | ? | - |
? | |
| 3.2.1.4 | birchwood xylan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | ? | - |
? | |
| 3.2.1.4 | carboxymethyl cellulose + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma | ? | - |
? | |
| 3.2.1.8 | avicel + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
| 3.2.1.8 | barley glucan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
| 3.2.1.8 | beechwood xylan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
| 3.2.1.8 | birchwood xylan + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
| 3.2.1.8 | carboxymethyl cellulose + H2O | fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 50 | - |
carboxymethyl cellulase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus and of the parental endoglucanase | Teleogryllus emma |
| 3.2.1.4 | 65 | - |
parental xylanase | Teleogryllus emma |
| 3.2.1.4 | 70 | - |
xylanase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma |
| 3.2.1.8 | 50 | - |
carboxymethyl cellulase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus and of the parental endoglucanase | Thermomyces lanuginosus |
| 3.2.1.8 | 65 | - |
parental xylanase | Thermomyces lanuginosus |
| 3.2.1.8 | 70 | - |
xylanase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 50 | - |
t1/2: 173 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Teleogryllus emma |
| 3.2.1.4 | 50 | - |
t1/2: 693 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Teleogryllus emma |
| 3.2.1.4 | 60 | - |
t1/2: 16 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Teleogryllus emma |
| 3.2.1.4 | 60 | - |
t1/2: 347 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Teleogryllus emma |
| 3.2.1.4 | 70 | - |
t1/2: 15 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Teleogryllus emma |
| 3.2.1.4 | 70 | - |
t1/2: 231 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Teleogryllus emma |
| 3.2.1.4 | 80 | - |
t1/2: 12 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Teleogryllus emma |
| 3.2.1.4 | 80 | - |
t1/2: 63 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Teleogryllus emma |
| 3.2.1.4 | 90 | - |
t1/2: 12 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Teleogryllus emma |
| 3.2.1.4 | 90 | - |
t1/2: 20 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Teleogryllus emma |
| 3.2.1.8 | 50 | - |
t1/2: 173 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 50 | - |
t1/2: 693 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 60 | - |
t1/2: 16 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 60 | - |
t1/2: 347 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 70 | - |
t1/2: 15 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 70 | - |
t1/2: 231 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 80 | - |
t1/2: 12 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 80 | - |
t1/2: 63 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 90 | - |
t1/2: 12 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, endoglucanase activity | Thermomyces lanuginosus |
| 3.2.1.8 | 90 | - |
t1/2: 20 min, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus, xylanase activity | Thermomyces lanuginosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 3.5 | - |
carboxymethyl cellulase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus and of the parental endoglucanase | Teleogryllus emma |
| 3.2.1.4 | 7 | - |
xylanase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Teleogryllus emma |
| 3.2.1.8 | 3.5 | - |
carboxymethyl cellulase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus and of the parental endoglucanase | Thermomyces lanuginosus |
| 3.2.1.8 | 7 | - |
xylanase activity of the fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus | Thermomyces lanuginosus |
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Release 2023.1 (January 2023)
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