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Literature summary extracted from

  • Basit, A.; Liu, J.; Miao, T.; Zheng, F.; Rahim, K.; Lou, H.; Jiang, W.
    Characterization of two endo-beta-1, 4-xylanases from Myceliophthora thermophila and their saccharification efficiencies, synergistic with commercial cellulase (2018), Front. Microbiol., 9, 233 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 Co2+ 20.6% activation at 1 mM Pseudoalteromonas sp. DY3
3.2.1.4 Mn2+ 28.8% activation at 1 mM Pseudoalteromonas sp. DY3
3.2.1.8 additional information the enzyme activity is not affected by EDTA Thermothelomyces thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 gene celX, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudoalteromonas sp. DY3
3.2.1.8 gene MYCTH_49824, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X33 under control of an AOX1 promoter Thermothelomyces thermophilus
3.2.1.8 gene MYCTH_56237, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X33 under control of an AOX1 promoter Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+ 6.5% inhibition at 1 mM Pseudoalteromonas sp. DY3
3.2.1.4 Pb2+ 14.9% inhibition at 1 mM Pseudoalteromonas sp. DY3
3.2.1.8 Cu2+
-
 Thermothelomyces thermophilus
3.2.1.8 additional information the enzyme activity is not affected by EDTA; the enzyme activity is not affected by EDTA Thermothelomyces thermophilus
3.2.1.8 Ni2+
-
 Thermothelomyces thermophilus
3.2.1.8 Zn2+
-
 Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information Km for carboxymethyl cellulose is 6.4 mg/ml, pH 5.5, 45°C, recombinant enzyme, Michaelis-Menten kinetics Pseudoalteromonas sp. DY3
3.2.1.8 additional information
-
 additional information Km is 5.67 mg/ml Thermothelomyces thermophilus
3.2.1.8 additional information
-
 additional information Km is 8.80 mg/ml Thermothelomyces thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 additional information no or poor effect by 1 mM of Mg2+, Ba2+, Zn2+, Ca2+, and Ni2+ Pseudoalteromonas sp. DY3
3.2.1.8 Mg2+ activates Thermothelomyces thermophilus
3.2.1.8 Mn2+ activates Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Pseudoalteromonas sp. DY3
-
-
-
3.2.1.8 Thermothelomyces thermophilus G2QDB9 i.e. Sporotrichum thermophile
-
3.2.1.8 Thermothelomyces thermophilus G2QIK8 i.e. Sporotrichum thermophile
-
3.2.1.8 Thermothelomyces thermophilus ATCC 42464 G2QDB9 i.e. Sporotrichum thermophile
-
3.2.1.8 Thermothelomyces thermophilus ATCC 42464 G2QIK8 i.e. Sporotrichum thermophile
-
3.2.1.8 Thermothelomyces thermophilus BCRC 31852 G2QDB9 i.e. Sporotrichum thermophile
-
3.2.1.8 Thermothelomyces thermophilus BCRC 31852 G2QIK8 i.e. Sporotrichum thermophile
-
3.2.1.8 Thermothelomyces thermophilus DSM 1799 G2QDB9 i.e. Sporotrichum thermophile
-
3.2.1.8 Thermothelomyces thermophilus DSM 1799 G2QIK8 i.e. Sporotrichum thermophile
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein MYCTH_49824 has one O-glycosylation site (at residue 40) and four N-glycosylation sites (resides 20, 33, 101, 107) Thermothelomyces thermophilus
3.2.1.8 glycoprotein MYCTH_56237 has two putative O-glycosylation sites (residues 35 and 37) and one N-glycosylation site (Asn22) Thermothelomyces thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Pseudoalteromonas sp. DY3
3.2.1.8 recombinant His-tagged enzyme from Pichia pastoris strain X33 by nickel affinity chromatography Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 1412.5
-
 purified recombinant enzyme, pH 7.0, 60°C Thermothelomyces thermophilus
3.2.1.8 1533.7
-
 purified recombinant enzyme, pH 6.0, 60°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Pseudoalteromonas sp. DY3 ?
-
 ?
3.2.1.8 additional information the enzyme significantly cooperates with a commercial cellulase, it perfoms saccharification of the pretreated corn stover Thermothelomyces thermophilus ?
-
 ?
3.2.1.8 additional information the enzyme significantly cooperates with a commercial cellulase, it perfoms saccharification of the pretreated corn stover Thermothelomyces thermophilus ATCC 42464 ?
-
 ?
3.2.1.8 additional information the enzyme significantly cooperates with a commercial cellulase, it perfoms saccharification of the pretreated corn stover Thermothelomyces thermophilus BCRC 31852 ?
-
 ?
3.2.1.8 additional information the enzyme significantly cooperates with a commercial cellulase, it perfoms saccharification of the pretreated corn stover Thermothelomyces thermophilus DSM 1799 ?
-
 ?
3.2.1.8 xylan + H2O RBB-xylan as substrate Thermothelomyces thermophilus ?
-
 ?
3.2.1.8 xylan + H2O RBB-xylan as substrate Thermothelomyces thermophilus ATCC 42464 ?
-
 ?
3.2.1.8 xylan + H2O RBB-xylan as substrate Thermothelomyces thermophilus BCRC 31852 ?
-
 ?
3.2.1.8 xylan + H2O RBB-xylan as substrate Thermothelomyces thermophilus DSM 1799 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 52200, recombinant His-tagged enzyme, SDS-PAGE Pseudoalteromonas sp. DY3

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CelX
-
 Pseudoalteromonas sp. DY3
3.2.1.4 endoglucanase
-
 Pseudoalteromonas sp. DY3
3.2.1.8 endo-beta-1, 4-xylanase
-
 Thermothelomyces thermophilus
3.2.1.8 MYCTH_49824
-
 Thermothelomyces thermophilus
3.2.1.8 MYCTH_56237
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 45
-
 recombinant enzyme Pseudoalteromonas sp. DY3
3.2.1.8 60
-
 recombinant enzyme Thermothelomyces thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 5 45 34.7% of maximal activity at 5°C, maximal activity at 45°C, rapid loss of activity above. The cold adaptation of enzyme CelX is possibly due to the presence of the unusually long linker between the catalytic module and the cellulose-binding domain Pseudoalteromonas sp. DY3

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 25 55 purified recombinant enzyme, very stable at 25°C, loss of about 13.2% activity at 35°C after 2 h. The enzyme retains 10.4% activity at 45°C after 2 h, and is completely inactivated at 55°C after 30 min Pseudoalteromonas sp. DY3
3.2.1.8 70
-
 purified recombinant enzyme, 30 min, 60% activity remaining Thermothelomyces thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 4.2
-
 carboxymethyl cellulose pH 5.5, 45°C, recombinant enzyme Pseudoalteromonas sp. DY3

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.5
-
 recombinant enzyme Pseudoalteromonas sp. DY3
3.2.1.8 6
-
 recombinant enzyme Thermothelomyces thermophilus
3.2.1.8 7
-
 recombinant enzyme Thermothelomyces thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 5 9 over 76.9% of maximal activity within this range, no activity at pH 4.0 and below, 59.2% and 42.6% of maximal activity at pH 10.0 and pH 11.0 respectively Pseudoalteromonas sp. DY3

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 4
-
 purified recombinant His-tagged enzyme, inactivation after 1 h Pseudoalteromonas sp. DY3
3.2.1.4 5 11 purified recombinant His-tagged enzyme, 120 h, 83.0% activity remaining, 66% at pH 11.0 after 120 h Pseudoalteromonas sp. DY3
3.2.1.8 2 12 purified recombinant enzyme, 60 min, room temperature, MYCTH_49824 retains nearly 70% activity Thermothelomyces thermophilus
3.2.1.8 2 12 purified recombinant enzyme, 60 min, room temperature, MYCTH_56237 retains nearly 70% activity Thermothelomyces thermophilus

General Information

EC Number General Information Comment Organism
3.2.1.8 evolution the enzyme belongs to the glycosyl hydrolase family 11, GH11 Thermothelomyces thermophilus
3.2.1.8 additional information multiple template-based enzyme structure homology modeling Thermothelomyces thermophilus