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Literature summary extracted from

  • Krocova, E.; Neradova, S.; Kupcik, R.; Janovska, S.; Bilkova, Z.; Heidingsfeld, O.
    PHO15 genes of Candida albicans and Candida parapsilosis encode HAD-type phosphatases dephosphorylating 2-phosphoglycolate (2019), FEMS Yeast Res., 19, foy112.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.18 expression in Escherichia coli Candida albicans
3.1.3.18 expression in Escherichia coli Candida parapsilosis

General Stability

EC Number General Stability Organism
3.1.3.18 free phosphatase rapidly precipitates under all the conditions tested Candida albicans
3.1.3.18 free phosphatase rapidly precipitates under all the conditions tested Candida parapsilosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.18 3.26
-
 2-Phosphoglycolate pH 8.0, 37°C Candida parapsilosis
3.1.3.18 3.47
-
 2-Phosphoglycolate pH 8.3, 42°C Candida albicans
3.1.3.18 14.48
-
 2-glycerol phosphate pH 8.3, 42°C Candida albicans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.18 Co2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida albicans
3.1.3.18 Co2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida parapsilosis
3.1.3.18 Mg2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida albicans
3.1.3.18 Mg2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida parapsilosis
3.1.3.18 Mn2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida albicans
3.1.3.18 Mn2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida parapsilosis
3.1.3.18 additional information divalent cation required, no cofactor: Cu2+ Candida albicans
3.1.3.18 additional information divalent cation required, no cofactor: Cu2+ Candida parapsilosis
3.1.3.18 Ni2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida albicans
3.1.3.18 Ni2+ cations tested, in decreasing order of efficiency: Mn2+, Co2+, Mg2+, Ni2+ Candida parapsilosis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.18 Candida albicans
-
-
-
3.1.3.18 Candida parapsilosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.18 1,3-dihydroxyacetone phosphate + H2O about 20% of the activity with 2-phosphoglycolate Candida parapsilosis 1,3-dihydroxyacetone + phosphate
-
 ?
3.1.3.18 2-glycerol phosphate + H2O about 17% of the activity with 2-phosphoglycolate Candida albicans glycerol + phosphate
-
 ?
3.1.3.18 2-Phosphoglycolate + H2O
-
 Candida albicans Glycolate + phosphate
-
 ?
3.1.3.18 2-Phosphoglycolate + H2O
-
 Candida parapsilosis Glycolate + phosphate
-
 ?
3.1.3.18 4-nitrophenyl phosphate + H2O
-
 Candida albicans 4-nitrophenol + phosphate
-
 ?
3.1.3.18 4-nitrophenyl phosphate + H2O
-
 Candida parapsilosis 4-nitrophenol + phosphate
-
 ?
3.1.3.18 glyceraldehyde-3-phosphate + H2O about 20% of the activity with 2-phosphoglycolate Candida albicans glyceraldehyde + phosphate
-
 ?
3.1.3.18 additional information no substrates: phosphopeptides, 4-nitrophenylsulfate Candida albicans ?
-
 ?
3.1.3.18 additional information no substrates: phosphopeptides, 4-nitrophenylsulfate Candida parapsilosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.18 Pho15
-
 Candida albicans
3.1.3.18 Pho15
-
 Candida parapsilosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.18 37
-
-
 Candida parapsilosis
3.1.3.18 42
-
-
 Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.18 8
-
-
 Candida parapsilosis
3.1.3.18 8.3
-
-
 Candida albicans