Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sutter, J.; Johnsen, U.; Schönheit, P.
    Characterization of a pentonolactonase involved in D-xylose and L-arabinose catabolism in the haloarchaeon Haloferax volcanii (2017), FEMS Microbiol. Lett., 364, fnx140 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.68 KCl highest activity in 1 M KCl Haloferax volcanii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.424
-
Haloferax volcanii
3.1.1.68 expression in Haloferax volcanii H1209 Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.15 10.9
-
D-xylono-gamma-lactone pH 7.5, 42°C Haloferax volcanii
3.1.1.15 37.1
-
L-arabinono-1,4-lactone pH 7.5, 42°C Haloferax volcanii
3.1.1.68 10.9
-
D-xylono-1,4-lactone pH 7.5, 42°C Haloferax volcanii
3.1.1.68 37.1
-
L-arabino-1,4-lactone pH 7.5, 42°C Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.424 Mg2+ 50 mM used in assay conditions Haloferax volcanii
3.1.1.15 CoCl2 1 mM, 150% compared to activity with 1 mM MgCl2 Haloferax volcanii
3.1.1.15 FeCl2 1 mM, 21% compared to activity with 1 mM MgCl2 Haloferax volcanii
3.1.1.15 KCl the enzyme showed the highest activity at about 1 M KCl and requires divalent cations for activity Haloferax volcanii
3.1.1.15 MgCl2 Km: 0.2 mM Haloferax volcanii
3.1.1.15 MnCl2 1 mM, 86% compared to activity with 1 mM MgCl2 Haloferax volcanii
3.1.1.15 NiCl2 1 mM, 21% compared to activity with 1 mM MgCl2 Haloferax volcanii
3.1.1.15 ZnCl2 1 mM, 14% compared to activity with 1 mM MgCl2 Haloferax volcanii
3.1.1.68 Co2+ 150% of the activity with Mg2+ Haloferax volcanii
3.1.1.68 Mg2+ Km value 0.2 mM Haloferax volcanii
3.1.1.68 Mn2+ 86% of the activity with Mg2+ Haloferax volcanii
3.1.1.68 additional information divalent cation required Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.15 28000
-
gel filtration Haloferax volcanii
3.1.1.68 28000
-
gel filtration Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.424 D-xylose + NADP+ Haloferax volcanii
-
D-xylono-gamma-lactone + NADPH + H+
-
?
1.1.1.424 D-xylose + NADP+ Haloferax volcanii H26
-
D-xylono-gamma-lactone + NADPH + H+
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O Haloferax volcanii
-
L-arabinonate + H+
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O Haloferax volcanii DSM 3757
-
L-arabinonate + H+
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O Haloferax volcanii ATCC 29605
-
L-arabinonate + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.424 Haloferax volcanii
-
-
-
1.1.1.424 Haloferax volcanii D4GP29
-
-
1.1.1.424 Haloferax volcanii DSM 3757 D4GP29
-
-
1.1.1.424 Haloferax volcanii H26
-
-
-
3.1.1.15 Haloferax volcanii D4GP31
-
-
3.1.1.15 Haloferax volcanii ATCC 29605 D4GP31
-
-
3.1.1.68 Haloferax volcanii D4GP31
-
-
3.1.1.68 Haloferax volcanii DSM 3757 D4GP31
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.424 Ni-NTA column chromatography, and Superdex 200 gel filtration Haloferax volcanii
3.1.1.15
-
Haloferax volcanii
3.1.1.68 recombinant protein Haloferax volcanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.424 6
-
pH 8.2, 42°C Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.424 D-xylose + NADP+
-
Haloferax volcanii D-xylono-1,4-lactone + NADPH + H+
-
?
1.1.1.424 D-xylose + NADP+
-
Haloferax volcanii DSM 3757 D-xylono-1,4-lactone + NADPH + H+
-
?
1.1.1.424 D-xylose + NADP+
-
Haloferax volcanii D-xylono-gamma-lactone + NADPH + H+
-
?
1.1.1.424 D-xylose + NADP+
-
Haloferax volcanii H26 D-xylono-gamma-lactone + NADPH + H+
-
?
3.1.1.15 D-galactono-gamma-lactone + H2O
-
Haloferax volcanii ?
-
?
3.1.1.15 D-galactono-gamma-lactone + H2O
-
Haloferax volcanii DSM 3757 ?
-
?
3.1.1.15 D-galactono-gamma-lactone + H2O
-
Haloferax volcanii ATCC 29605 ?
-
?
3.1.1.15 D-glucono-delta-lactone + H2O
-
Haloferax volcanii ?
-
?
3.1.1.15 D-glucono-delta-lactone + H2O
-
Haloferax volcanii DSM 3757 ?
-
?
3.1.1.15 D-glucono-delta-lactone + H2O
-
Haloferax volcanii ATCC 29605 ?
-
?
3.1.1.15 D-xylono-gamma-lactone + H2O
-
Haloferax volcanii xylonate
-
?
3.1.1.15 D-xylono-gamma-lactone + H2O
-
Haloferax volcanii DSM 3757 xylonate
-
?
3.1.1.15 D-xylono-gamma-lactone + H2O
-
Haloferax volcanii ATCC 29605 xylonate
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O
-
Haloferax volcanii L-arabinonate
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O
-
Haloferax volcanii DSM 3757 L-arabinonate
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O
-
Haloferax volcanii ATCC 29605 L-arabinonate
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O
-
Haloferax volcanii L-arabinonate + H+
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O
-
Haloferax volcanii DSM 3757 L-arabinonate + H+
-
?
3.1.1.15 L-arabinono-1,4-lactone + H2O
-
Haloferax volcanii ATCC 29605 L-arabinonate + H+
-
?
3.1.1.68 D-xylono-1,4-lactone + H2O
-
Haloferax volcanii D-xylonate
-
?
3.1.1.68 L-arabino-1,4-lactone + H2O
-
Haloferax volcanii L-arabinonate
-
?
3.1.1.68 L-galactono-1,4-lactone + H2O
-
Haloferax volcanii L-galactonate
-
?
3.1.1.68 L-glucono-1,4-lactone + H2O
-
Haloferax volcanii L-gluconate
-
?
3.1.1.68 additional information no substrate: D-ribono-1,4 -lactone Haloferax volcanii ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.68 monomer 1 * 31400, calculated from sequence, 1 * 35000, SDS-PAGE Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.424 D-xylose dehydrogenase
-
Haloferax volcanii
1.1.1.424 XDH
-
Haloferax volcanii
3.1.1.15 XacC
-
Haloferax volcanii
3.1.1.68 HVO B0030
-
Haloferax volcanii
3.1.1.68 XacC
-
Haloferax volcanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.15 42
-
assay at Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.15 7.5
-
assay at Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.424 NADP+
-
Haloferax volcanii

Expression

EC Number Organism Comment Expression
3.1.1.15 Haloferax volcanii the enzyme is transcriptionally upregulated by both D-xylose and L-arabinose mediated by the pentose-specific regulator XacR up
3.1.1.68 Haloferax volcanii expression is upregulated by both D-xylose and L-arabinose mediated by the pentose-specific regulator XacR up

General Information

EC Number General Information Comment Organism
1.1.1.424 physiological function Haloferax volcanii degrades the pentoses D-xylose and L-arabinose via an oxidative pathway to 2-oxoglutarate as an intermediate. The initial dehydrogenases of the pathway, D-xylose dehydrogenase (XDH) and L-arabinose dehydrogenase (L-AraDH) catalyze the NADP+ dependent D-xylose and L-arabinose oxidation Haloferax volcanii
3.1.1.15 metabolism the enzyme is involved in the oxidative pathway of L-arabinose degradation to alpha-ketoglutarate Haloferax volcanii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.15 10.74
-
L-arabinono-1,4-lactone pH 7.5, 42°C Haloferax volcanii
3.1.1.15 20.96
-
D-xylono-gamma-lactone pH 7.5, 42°C Haloferax volcanii
3.1.1.68 10.74
-
L-arabino-1,4-lactone pH 7.5, 42°C Haloferax volcanii
3.1.1.68 20.9
-
D-xylono-1,4-lactone pH 7.5, 42°C Haloferax volcanii