Literature summary extracted from

Adhav, A.; Harne, S.; Bhide, A.; Giri, A.; Gayathri, P.; Joshi, R.
Mechanistic insights into enzymatic catalysis by trehalase from the insect gut endosymbiont Enterobacter cloacae (2019), FEBS J., 286, 1700-1716 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.28	expression in Escherichia coli	Enterobacter cloacae

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.2.1.28	crystal structures are determined in the ligand-free form and bound to the inhibitor validoxylamine A. Multiple salt bridges are identified that potentially facilitate closure of a hood over the substrate-binding site	Enterobacter cloacae

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.2.1.28	validoxylamine A	competitive inhibition	Enterobacter cloacae

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.1.28	1.47	-	alpha,alpha-trehalose	55°C, pH 5.0	Enterobacter cloacae

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.2.1.28	periplasm	-	Enterobacter cloacae	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.2.1.28	65000	-	SDS-PAGE	Enterobacter cloacae

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.2.1.28	alpha,alpha-trehalose + H2O	Enterobacter cloacae	-	beta-D-glucose + alpha-D-glucose	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.28	Enterobacter cloacae	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.28	-	Enterobacter cloacae

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.28	alpha,alpha-trehalose + H2O	-	Enterobacter cloacae	beta-D-glucose + alpha-D-glucose	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.28	Px_EclTre	-	Enterobacter cloacae

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.2.1.28	55	-	-	Enterobacter cloacae

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2.1.28	104.25	-	alpha,alpha-trehalose	55°C, pH 5.0	Enterobacter cloacae

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.2.1.28	5	-	-	Enterobacter cloacae

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.2.1.28	0.0000019	-	validoxylamine A	55°C, pH 5.0	Enterobacter cloacae

IC50 Value

EC Number	IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
3.2.1.28	0.0000075	-	55°C, pH 5.0	Enterobacter cloacae	validoxylamine A

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Release 2023.1 (January 2023)