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Literature summary extracted from

  • Lim, S.M.; Yeung, K.; Tresaugues, L.; Ling, T.H.; Nordlund, P.
    The structure and catalytic mechanism of human sphingomyelin phosphodiesterase like 3a--an acid sphingomyelinase homologue with a novel nucleotide hydrolase activity (2016), FEBS J., 283, 1107-1123 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expressed in Sf9 insect cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.12 catalytic domain in complex with CMP, sitting drop vapor diffusion method, using 2.08 M disodium malonate pH 5, 0.23 M sodium thiocyanate Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 Zn2+ high millimolar Zn2+ concentration leads to inhibition of enzyme activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.262
-
CDP-choline at pH 5.0 and 37°C Homo sapiens
3.1.4.12 0.306
-
ADP at pH 5.0 and 37°C Homo sapiens
3.1.4.12 0.327
-
ATP at pH 5.0 and 37°C Homo sapiens
3.1.4.12 0.348
-
ADP-ribose at pH 5.0 and 37°C Homo sapiens
3.1.4.12 0.391
-
CDP-ethanolamine at pH 5.0 and 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Zn2+ contains two zinc ions Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens Q92484
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.12 glycoprotein the enzyme has seven sites for glycosylation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 Ni-NTA column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 ADP + H2O
-
Homo sapiens ?
-
?
3.1.4.12 ADP-ribose + H2O
-
Homo sapiens ?
-
?
3.1.4.12 ATP + H2O
-
Homo sapiens ?
-
?
3.1.4.12 CDP-choline + H2O
-
Homo sapiens CMP + phosphocholine
-
?
3.1.4.12 CDP-ethanolamine + H2O
-
Homo sapiens CMP + phosphoethanolamine
-
?
3.1.4.12 additional information no activity with sphingomyelin or AMP Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.4.12 monomer 1 * 59400, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 SMPDL3A
-
Homo sapiens
3.1.4.12 sphingomyelin phosphodiesterase like 3a
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.12 0.374
-
ADP at pH 5.0 and 37°C Homo sapiens
3.1.4.12 0.7357
-
ATP at pH 5.0 and 37°C Homo sapiens
3.1.4.12 1.29
-
CDP-choline at pH 5.0 and 37°C Homo sapiens
3.1.4.12 1.864
-
CDP-ethanolamine at pH 5.0 and 37°C Homo sapiens
3.1.4.12 2.07
-
ADP-ribose at pH 5.0 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.12 1.23
-
ADP at pH 5.0 and 37°C Homo sapiens
3.1.4.12 2.26
-
ATP at pH 5.0 and 37°C Homo sapiens
3.1.4.12 4.89
-
CDP-choline at pH 5.0 and 37°C Homo sapiens
3.1.4.12 5.71
-
CDP-ethanolamine at pH 5.0 and 37°C Homo sapiens
3.1.4.12 5.96
-
ADP-ribose at pH 5.0 and 37°C Homo sapiens