EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.4 | cloned from camel rumen metagenom, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | uncultured bacterium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.4 | acetone | - |
uncultured bacterium | |
3.2.1.4 | Benzene | - |
uncultured bacterium | |
3.2.1.4 | chloroform | - |
uncultured bacterium | |
3.2.1.4 | Cyclohexane | - |
uncultured bacterium | |
3.2.1.4 | ethanol | - |
uncultured bacterium | |
3.2.1.4 | guanidinium hydrochloride | 72.2% at 3% | uncultured bacterium | |
3.2.1.4 | isobutanol | - |
uncultured bacterium | |
3.2.1.4 | Isopropanol | - |
uncultured bacterium | |
3.2.1.4 | methanol | - |
uncultured bacterium | |
3.2.1.4 | additional information | organic solvents at 10-20% | uncultured bacterium | |
3.2.1.4 | n-hexane | - |
uncultured bacterium | |
3.2.1.4 | PMSF | 46.7% at 5 mM | uncultured bacterium | |
3.2.1.4 | SDS | complete inhibition at 0.1% | uncultured bacterium | |
3.2.1.4 | Toluene | - |
uncultured bacterium | |
3.2.1.4 | Tween 20 | 31% inhibition at 20% | uncultured bacterium | |
3.2.1.4 | Urea | 82.3% inhibition at 10%, 42.3% at 7%, and 24.3 at 5% | uncultured bacterium | |
3.2.1.4 | Zn2+ | about 60-80% inhibition at 5-10 mM | uncultured bacterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.4 | additional information | - |
additional information | Vmax and Km are 339 U/mg and 2.57 mg/ml, respectively, Michaelis-Menten kinetics and thermodynamic analysis, overview | uncultured bacterium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.4 | Cd2+ | activates slightly at 10 mM | uncultured bacterium | |
3.2.1.4 | Co2+ | activates at 5-10 mM | uncultured bacterium | |
3.2.1.4 | Mn2+ | activates at 5-10 mM | uncultured bacterium | |
3.2.1.4 | Na+ | activates at 5 mM, not at 10 mM | uncultured bacterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.4 | uncultured bacterium | A0A1W5YNV1 | isolated from camel rumen | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.4 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | uncultured bacterium |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.4 | carboxymethyl cellulose + H2O | - |
uncultured bacterium | ? | - |
? | |
3.2.1.4 | additional information | no activity with starch | uncultured bacterium | ? | - |
? | |
3.2.1.4 | oat spelt xylan + H2O | - |
uncultured bacterium | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.4 | ? | x * 46500, about, sequence calculation | uncultured bacterium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.4 | CelCM3 | - |
uncultured bacterium |
3.2.1.4 | cellulase CM3 | - |
uncultured bacterium |
3.2.1.4 | endoglucanase | - |
uncultured bacterium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 30 | - |
- |
uncultured bacterium |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 10 | 60 | optimum temperature is 30°C, over 60% of maximal activity in the range of 10-50°C, 13% at 60°C, no activity at 70-80°C, profile overview | uncultured bacterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 4 | 45 | the enzyme has an estimated low melting temperature of 45°C, about 50% activity remaining at 4°C, it is identified to be cold-adapted | uncultured bacterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 5 | - |
- |
uncultured bacterium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 3 | 9 | activity range, over 50% of maximal activity at pH 4.0-7.5, profile overview | uncultured bacterium |