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Literature summary extracted from

  • Berto, G.L.; Velasco, J.; Tasso Cabos Ribeiro, C.; Zanphorlin, L.M.; Noronha Domingues, M.; Tyago Murakami, M.; Polikarpov, I.; de Oliveira, L.C.; Ferraz, A.; Segato, F.
    Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45 (2019), Enzyme Microb. Technol., 120, 23-35 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.4 industry endoglucanases from family GH45 are applied in formulation of detergents and in industrial pulp and paper processes Gloeophyllum trabeum
3.2.1.4 industry endoglucanases from family GH45 are applied in formulation of detergents and in industrial pulp and paper processes Thermothelomyces thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 phylogenetic analysis and tree Gloeophyllum trabeum
3.2.1.4 phylogenetic analysis and tree Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+ slight inhibition Gloeophyllum trabeum
3.2.1.4 Cu2+ strong inhibition Thermothelomyces thermophilus
3.2.1.4 EDTA slight inhibition Gloeophyllum trabeum
3.2.1.4 Fe2+ complete inhibition Gloeophyllum trabeum
3.2.1.4 Fe2+ complete inhibition Thermothelomyces thermophilus
3.2.1.4 Mg2+ slight inhibition Gloeophyllum trabeum
3.2.1.4 Mn2+ strong inhibition Thermothelomyces thermophilus
3.2.1.4 Na+ slight inhibition Gloeophyllum trabeum
3.2.1.4 Na+ slight inhibition Thermothelomyces thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Co2+ 50.9% activation Thermothelomyces thermophilus
3.2.1.4 Co2+ 87.8% activation Gloeophyllum trabeum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Gloeophyllum trabeum
-
-
-
3.2.1.4 Thermothelomyces thermophilus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 1.3
-
 purified enzyme MtGH45, substrate phosphoric acid swollen cellulose, pH 6.5, 50°C Thermothelomyces thermophilus
3.2.1.4 3
-
 purified enzyme MtGH45, substrate Avicel, pH 6.5, 50°C Thermothelomyces thermophilus
3.2.1.4 3.3
-
 purified enzyme GtGH45, substrate phosphoric acid swollen cellulose, pH 6.5, 50°C Gloeophyllum trabeum
3.2.1.4 8.5
-
 purified enzyme GtGH45, substrate cotton, pH 6.5, 50°C Gloeophyllum trabeum
3.2.1.4 8.8
-
 purified enzyme GtGH45, substrate Avicel, pH 6.5, 50°C Gloeophyllum trabeum
3.2.1.4 12.8
-
 purified enzyme MtGH45, substrate cotton, pH 6.5, 50°C Thermothelomyces thermophilus
3.2.1.4 35.6
-
 purified enzyme MtGH45, substrate lichenan, pH 6.5, 50°C Thermothelomyces thermophilus
3.2.1.4 52.5
-
 purified enzyme MtGH45, substrate beta-glucan, pH 6.5, 50°C Thermothelomyces thermophilus
3.2.1.4 54.2
-
 purified enzyme GtGH45, substrate lichenan, pH 6.5, 50°C Gloeophyllum trabeum
3.2.1.4 170.7
-
 purified enzyme GtGH45, substrate beta-glucan, pH 6.5, 50°C Gloeophyllum trabeum
3.2.1.4 200.6
-
 purified enzyme GtGH45, substrate carboxymethyl cellulose, pH 6.5, 50°C Gloeophyllum trabeum
3.2.1.4 202.6
-
 purified enzyme MtGH45, substrate carboxymethyl cellulose, pH 6.5, 50°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
 Gloeophyllum trabeum ?
-
 ?
3.2.1.4 avicel + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Gloeophyllum trabeum ?
-
 ?
3.2.1.4 beta-glucan + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O best substrate Gloeophyllum trabeum ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O best substrate Thermothelomyces thermophilus ?
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Gloeophyllum trabeum D-glucose + ?
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Thermothelomyces thermophilus cellobiose + cellotriose + cellotetraose
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Gloeophyllum trabeum D-glucose + ?
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Thermothelomyces thermophilus cellobiose + cellotriose
-
 ?
3.2.1.4 cellotetraose + H2O
-
 Gloeophyllum trabeum D-glucose + cellobiose + cellotriose
-
 ?
3.2.1.4 cellotriose + H2O
-
 Gloeophyllum trabeum D-glucose + cellobiose
-
 ?
3.2.1.4 cotton + H2O
-
 Gloeophyllum trabeum ?
-
 ?
3.2.1.4 cotton + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Gloeophyllum trabeum ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.2.1.4 additional information substrate specificity, overview. Enzyme MtGH45 is unable to hydrolyze C3 and C4 oligosaccharides, cellobiose and cellotriose are detected as the main products when the enzyme is incubated with cellopentaose, which is completely hydrolyzed and the incubation with cellohexaose generates cellobiose, cellotriose, and cellotetraose as main products Thermothelomyces thermophilus ?
-
 ?
3.2.1.4 additional information substrate specificity, overview. The GtGH45 is able to convert all tested oligosaccharides to glucose, however, small amounts of cellobiose, cellotriose, and cellotetraose are detected in the reaction products, indicating that the enzyme cannot convert efficiently small oligosaccharides probably due their mode of recognition in the enzyme catalytic cleft. The degradation of C5 and C6 by GtGH45 is almost complete with glucose being a main product Gloeophyllum trabeum ?
-
 ?
3.2.1.4 phosphoric acid swollen cellulose + H2O
-
 Gloeophyllum trabeum ?
-
 ?
3.2.1.4 phosphoric acid swollen cellulose + H2O
-
 Thermothelomyces thermophilus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 endoglucanase
-
 Gloeophyllum trabeum
3.2.1.4 endoglucanase
-
 Thermothelomyces thermophilus
3.2.1.4 GH45 endoglucanase
-
 Gloeophyllum trabeum
3.2.1.4 GH45 endoglucanase
-
 Thermothelomyces thermophilus
3.2.1.4 GtGH45
-
 Gloeophyllum trabeum
3.2.1.4 MtGH45
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 65
-
-
 Gloeophyllum trabeum
3.2.1.4 65
-
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 66
-
 melting temperature of enzyme GtGH45 Gloeophyllum trabeum
3.2.1.4 80.9
-
 melting temperature of enzyme MtGH45 Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
-
 Gloeophyllum trabeum
3.2.1.4 5
-
-
 Thermothelomyces thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4 6 over 50% of maximal activity within this range Gloeophyllum trabeum
3.2.1.4 4.5 10 over 50% of maximal activity within this range Thermothelomyces thermophilus

General Information

EC Number General Information Comment Organism
3.2.1.4 evolution the enzyme belongs to the glycosyl hydrolase family 45, GH45, subfamily GH45A Thermothelomyces thermophilus
3.2.1.4 evolution the enzyme belongs to the glycosyl hydrolase family 45, GH45, subfamily GH45C Gloeophyllum trabeum
3.2.1.4 additional information molecular docking of enzyme GtGH45 with cellooligosaccharides cellopentaose, cellotetraose, cellotriose, and cellobiose Gloeophyllum trabeum
3.2.1.4 additional information the molecular docking simulations of MtGH45 reveal the requirements of the sites -2, -1, +1, +2 and +3 to be occupied for hydrolysis. Molecular docking of C4, C3 and C2 is unproductive Thermothelomyces thermophilus