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Literature summary extracted from

  • Yan, R.; Vuong, T.V.; Wang, W.; Master, E.R.
    Action of a GH115 alpha-glucuronidase from Amphibacillus xylanus at alkaline condition promotes release of 4-O-methylglucopyranosyluronic acid from glucuronoxylan and arabinoglucuronoxylan (2017), Enzyme Microb. Technol., 104, 22-28 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.131 expression in Escherichia coli BL21 with an N-terminal His tag Amphibacillus xylanus
3.2.1.131 the enzyme is expressed in Escherichia coli BL21 (DE3) CodonPlus in fusion with an N-terminal His tag Amphibacillus xylanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.131 Ag+ 1 mM, 94% loss of activity Amphibacillus xylanus
3.2.1.131 AgNO3 1 mM, 94% loss of activity Amphibacillus xylanus
3.2.1.131 Cu2+ 1 mM, 49% loss of activity Amphibacillus xylanus
3.2.1.131 CuSO4 1 mM, 49% loss of activity Amphibacillus xylanus
3.2.1.131 additional information addition of 1 mM Mg2+, Ca2+, Fe3+ or Zn2+ has no effect Amphibacillus xylanus
3.2.1.131 Ni2+ 1 mM, 12% loss of activity Amphibacillus xylanus
3.2.1.131 NiCl2 1 mM, 12% loss of activity Amphibacillus xylanus
3.2.1.131 Sr2+ 1 mM, 17% loss of activity Amphibacillus xylanus
3.2.1.131 SrCl2 1 mM, 17% loss of activity Amphibacillus xylanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.131 additional information
-
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose Km-value: 2.4 mg/ml, pH 7.0, 55°C Amphibacillus xylanus
3.2.1.131 additional information
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-D-xylose Km-value: 5.0 mg/ml, pH 7.0, 55°C Amphibacillus xylanus
3.2.1.131 additional information
-
arabinoglucuronoxylan Km-value: 50 mg/ml, pH 7.0, 55°C Amphibacillus xylanus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.131 additional information 1 mM Mg2+, Ca2+, Fe3+ or Zn2+ has no effect on activity Amphibacillus xylanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.131 109960
-
calculated from sequence Amphibacillus xylanus
3.2.1.131 110000
-
SDS-PAGE Amphibacillus xylanus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.131 Amphibacillus xylanus K0J4X5
-
-
3.2.1.131 Amphibacillus xylanus ATCC 51415 K0J4X5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.131
-
Amphibacillus xylanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.131 4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-D-xylose + H2O
-
Amphibacillus xylanus 4-O-methyl-glucuronic acid + ?
-
?
3.2.1.131 4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-D-xylose + H2O
-
Amphibacillus xylanus ATCC 51415 4-O-methyl-glucuronic acid + ?
-
?
3.2.1.131 4-O-methyl-alpha-D-glucopyranosyl-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O
-
Amphibacillus xylanus 4-O-methyl-glucuronic acid + ?
-
?
3.2.1.131 4-O-methyl-alpha-D-glucopyranosyl-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose + H2O
-
Amphibacillus xylanus ATCC 51415 4-O-methyl-glucuronic acid + ?
-
?
3.2.1.131 arabinoglucuronoxylan + H2O
-
Amphibacillus xylanus 4-O-methyl-glucuronic acid + ?
-
?
3.2.1.131 arabinoglucuronoxylan + H2O
-
Amphibacillus xylanus ATCC 51415 4-O-methyl-glucuronic acid + ?
-
?
3.2.1.131 beechwood xylan + H2O 83% of the activity as compared to spruce arabinoglucuronoxylan Amphibacillus xylanus 4-O-methyl-D-glucuronic acid + ?
-
?
3.2.1.131 beechwood xylan + H2O 83% of the activity as compared to spruce arabinoglucuronoxylan Amphibacillus xylanus ATCC 51415 4-O-methyl-D-glucuronic acid + ?
-
?
3.2.1.131 glucuronoxylan + H2O
-
Amphibacillus xylanus 4-O-methyl-glucuronic acid + ?
-
?
3.2.1.131 additional information the enzyme is similarly active towards glucuronoxylan as well as comparatively complex xylans such as spruce arabinoglucurunoxylan Amphibacillus xylanus ?
-
?
3.2.1.131 additional information the enzyme is similarly active towards glucuronoxylan as well as comparatively complex xylans such as spruce arabinoglucurunoxylan Amphibacillus xylanus ATCC 51415 ?
-
?
3.2.1.131 oat spelt xylan + H2O 8.9% of the activity as compared to spruce arabinoglucuronoxylan Amphibacillus xylanus 4-O-methyl-D-glucuronic acid + ?
-
?
3.2.1.131 spruce arabinoglucuronoxylan + H2O
-
Amphibacillus xylanus 4-O-methyl-D-glucuronic acid + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.131 ? x * 110000, SDS-PAGE Amphibacillus xylanus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.131 AxyAgu115A
-
Amphibacillus xylanus
3.2.1.131 GH115 alpha-glucuronidase
-
Amphibacillus xylanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.131 55
-
-
Amphibacillus xylanus
3.2.1.131 55
-
assay at Amphibacillus xylanus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.131 40
-
half life (t1/2): 24 h Amphibacillus xylanus
3.2.1.131 40
-
half life: 24 h Amphibacillus xylanus
3.2.1.131 45
-
1 h, enzyme retains 100% activity. Half life: 10 h Amphibacillus xylanus
3.2.1.131 45
-
the enzyme retains 100% activity following preincubation for 1 h. Half life (t1/2): 10 h Amphibacillus xylanus
3.2.1.131 50
-
1 h, enzyme retains 75% of its initial activity. Half life: 90 min Amphibacillus xylanus
3.2.1.131 50
-
the enzyme retains 75% activity following preincubation for 1 h. Half life (t1/2): 90 min Amphibacillus xylanus
3.2.1.131 55
-
1 h, complete loss of activity. Half life: 7 min Amphibacillus xylanus
3.2.1.131 55
-
no activity is detected after incubation for 1 h at temperatures above 55°C. Half life (t1/2): 7 min Amphibacillus xylanus
3.2.1.131 60
-
half life (t1/2): 5 min Amphibacillus xylanus
3.2.1.131 60
-
half life: 5 min Amphibacillus xylanus
3.2.1.131 65
-
half life (t1/2): 2 min Amphibacillus xylanus
3.2.1.131 65
-
half life: 2 min Amphibacillus xylanus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.131 29.2
-
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-D-xylose pH 7.0, 55°C Amphibacillus xylanus
3.2.1.131 72.7
-
4-O-methyl-alpha-D-glucopyranosyl-(1->2)-[beta-D-xylopyranosyl-(1->4)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylopyranose pH 7.0, 55°C Amphibacillus xylanus
3.2.1.131 487.3
-
arabinoglucuronoxylan pH 7.0, 55°C Amphibacillus xylanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.131 7
-
-
Amphibacillus xylanus
3.2.1.131 7
-
activity on beechwood xylan Amphibacillus xylanus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.131 5 8.5 the enzyme retains over 80% relative activity between pH 5.0 to 8.5 Amphibacillus xylanus
3.2.1.131 5 10.5 relative activity between pH 5.0 and pH 8.5 was above 80%, and was 35% of maximum at pH 10.5, activity on beechwood xylan Amphibacillus xylanus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.131 9
-
the enzyme loses 30% and 80% relative residual activity after 24 h preincubation Amphibacillus xylanus
3.2.1.131 10
-
the enzyme loses 30% and 80% relative residual activity after 24 h preincubation Amphibacillus xylanus