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Literature summary extracted from

  • Mohamed, S.; Bouacem, K.; Mechri, S.; Addou, N.A.; Laribi-Habchi, H.; Fardeau, M.L.; Jaouadi, B.; Bouanane-Darenfed, A.; Hacene, H.
    Purification and biochemical characterization of a novel acido-halotolerant and thermostable endochitinase from Melghiribacillus thermohalophilus strain Nari2AT (2019), Carbohydr. Res., 473, 46-56 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.14 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 110% activity at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 chloroform about 160% activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 Cyclohexane about 148% activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 dimethylformamide about % activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 DMF about % activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 DMSO about 160% activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 Isopropanol about 150% activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 n-decane about 110% activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 n-hexane about 130% activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 NaCl maximum activity at 4 M Melghiribacillus thermohalophilus

General Stability

EC Number General Stability Organism
3.2.1.14 4 M NaCl. Beyond 4M NaCl, chitinase activity decreases progressively and retains 30% of its relative activity at 10 M NaCl Melghiribacillus thermohalophilus
3.2.1.14 more than 30% of residual activities are obtained at the range of 0.5-10 M NaCl with optimal activity reached at Melghiribacillus thermohalophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 2-mercaptoethanol 20% residual activity at 10 mM Melghiribacillus thermohalophilus
3.2.1.14 5,5'-dithio-bis(2-nitrobenzoic acid) 2% residual activity at 10 mM Melghiribacillus thermohalophilus
3.2.1.14 acetonitrile complete inhibition at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 Ag+ 10% residual activity at 10 mM Melghiribacillus thermohalophilus
3.2.1.14 Al3+ 73% residual activity at 10 mM Melghiribacillus thermohalophilus
3.2.1.14 Ba2+ 96% residual activity at 10 mM Melghiribacillus thermohalophilus
3.2.1.14 Cd2+ complete inhibition at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 Cu2+ 51% residual activity at 10 mM Melghiribacillus thermohalophilus
3.2.1.14 dithiothreitol 8% residual activity at 10 mM Melghiribacillus thermohalophilus
3.2.1.14 ethyl acetate complete inhibition at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 Fe2+ 32% residual activity at 10 mM Melghiribacillus thermohalophilus
3.2.1.14 Hg2+ complete inhibition at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 isooctane about 90% residual activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 additional information not inhibited by 2,4,6-trinitrobenzenesulfonic acid, phenylmethylsulfonyl fluoride, diethyldicarbonate, and N-bromosuccinimide Melghiribacillus thermohalophilus
3.2.1.14 N-Acetylimidazole 94% residual activity at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 N-ethylmaleimide complete inhibition at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 n-heptane about 80% residual activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 n-hexadecane about 70% residual activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 Ni2+ complete inhibition at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 p-chloromercuribenzoic acid complete inhibition at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 Toluene about 80% residual activity at 25%(v/v) Melghiribacillus thermohalophilus
3.2.1.14 Zn2+ 56% residual activity at 10 mM Melghiribacillus thermohalophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Ca2+ 280% activity at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 Mg2+ 175% activity at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 Mn2+ 127% activity at 2 mM Melghiribacillus thermohalophilus
3.2.1.14 additional information not influenced by Co2+ Melghiribacillus thermohalophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Melghiribacillus thermohalophilus
-
-
-
3.2.1.14 Melghiribacillus thermohalophilus Nari2AT
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 UNO S-1 column chromatography and Zorbax PSM 300 HPSEC column chromatography Melghiribacillus thermohalophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-D- N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O 535% activity compared to colloidal chitin Melghiribacillus thermohalophilus 4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide
-
 ?
3.2.1.14 4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O 198% activity compared to colloidal chitin Melghiribacillus thermohalophilus 4-nitrophenyl beta-D-N,N'-diacetylchitobioside
-
 ?
3.2.1.14 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O 150% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
3.2.1.14 beta-D-N,N',N''-triacetylchitotriose + H2O 86% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
3.2.1.14 chitin azure + H2O 97% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
3.2.1.14 chitin azure + H2O 97% activity compared to colloidal chitin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?
3.2.1.14 chitooligosaccharide + H2O 77% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
3.2.1.14 colloidal chitin + H2O 100% activity Melghiribacillus thermohalophilus N-acetyl-beta-D-glucosamine + ?
-
 ?
3.2.1.14 glycol chitin + H2O 92% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
3.2.1.14 glycol chitin + H2O 92% activity compared to colloidal chitin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?
3.2.1.14 glycol chitosan + H2O 88% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
3.2.1.14 glycol chitosan + H2O 88% activity compared to colloidal chitin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?
3.2.1.14 additional information the enzyme does not hydrolyze 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, beta-D-N,N'-diacetylchitobiose, amylose, and laminarin Melghiribacillus thermohalophilus ?
-
 ?
3.2.1.14 additional information the enzyme does not hydrolyze 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, 4-nitrophenyl N-acetyl-beta-D-glucosaminide, beta-D-N,N'-diacetylchitobiose, amylose, and laminarin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?
3.2.1.14 xylan + H2O 40% activity compared to colloidal chitin Melghiribacillus thermohalophilus ?
-
 ?
3.2.1.14 xylan + H2O 40% activity compared to colloidal chitin Melghiribacillus thermohalophilus Nari2AT ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 45000, SDS-PAGE Melghiribacillus thermohalophilus
3.2.1.14 monomer 1 * 45201, calculated from amino acid sequence Melghiribacillus thermohalophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiA-Mt45
-
 Melghiribacillus thermohalophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 90
-
-
 Melghiribacillus thermohalophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 3.5
-
-
 Melghiribacillus thermohalophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 2 6 the half-life times of the enzyme at pH 2.0, 3.0, 4.0, 5.0, and 6.0 are 18, 14, 10, 6, and 2 h, respectively Melghiribacillus thermohalophilus