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Literature summary extracted from
- Thermostability enhancement of chitosanase CsnA by fusion a family 5 carbohydrate-binding module (2017), Biotechnol. Lett., 39, 1895-1901 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.132 | industry | the fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) exhibits higher chitosan binding capacity and catalytic activity than wild-type enzyme and improved thermostability. The hybrid enzyme is a useful candidate for industrial application | Renibacterium sp. QD1 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.132 | expression in Escherichia coli BL21(DE3) | Renibacterium sp. QD1 |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.132 | additional information | the fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) exhibits higher chitosan binding capacity and catalytic activity than wild-type enzyme. The fusion of BgCBM5 significantly improves the thermostability of citosanase CsnA | Renibacterium sp. QD1 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.132 | Al3+ | 1 mM, 86.5% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM, 87% inhibition of wild-type enzyme | Renibacterium sp. QD1 |  |
| 3.2.1.132 | Cu2+ | 1 mM, complete inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) and of wild-type enzyme | Renibacterium sp. QD1 | |
| 3.2.1.132 | Fe3+ | 1 mM, 11% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM, 13% inhibition of wild-type enzyme | Renibacterium sp. QD1 | |
| 3.2.1.132 | Ni2+ | 1 mM, 22% inhibition of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5). 1 mM,10% inhibition of wild-type enzyme | Renibacterium sp. QD1 | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.132 | additional information | - |
chitosan | KM-value: 31.5 g/l, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35), pH 5.6, 55°C | Renibacterium sp. QD1 | |
| 3.2.1.132 | additional information | - |
chitosan | KM-value: 42 g/l, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2), pH 5.6, 55°C | Renibacterium sp. QD1 | |
| 3.2.1.132 | additional information | - |
chitosan | KM-value: 43 g/l, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), pH 5.6, 55°C | Renibacterium sp. QD1 | |
| 3.2.1.132 | additional information | - |
chitosan | KM-value: 69 g/l, wild-type enzyme, pH 5.6, 55°C | Renibacterium sp. QD1 | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.132 | Mn2+ | 1 mM, 1.5fold activation of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), 1.9fold activation of wild-type enzyme | Renibacterium sp. QD1 | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.132 | Renibacterium sp. QD1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.132 | - |
Renibacterium sp. QD1 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.132 | chitosan + H2O | colloidal chitosan | Renibacterium sp. QD1 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.132 | chitosanase CsnA | - |
Renibacterium sp. QD1 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 50 | - |
wild-type enzyme and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35) | Renibacterium sp. QD1 |
| 3.2.1.132 | 55 | - |
fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2) | Renibacterium sp. QD1 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 48.9 | - |
T50-value, wild-type enzyme | Renibacterium sp. QD1 |
| 3.2.1.132 | 57.8 | - |
T50-value, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) | Renibacterium sp. QD1 |
| 3.2.1.132 | 65 | - |
1 h, wild-type enzyme and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5) retain 37% activity. Complete loss of activity of fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2) and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35) | Renibacterium sp. QD1 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.132 | 20800 | - |
chitosan | fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35), pH 5.6, 55°C | Renibacterium sp. QD1 | |
| 3.2.1.132 | 30100 | - |
chitosan | fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2), pH 5.6, 55°C | Renibacterium sp. QD1 | |
| 3.2.1.132 | 64400 | - |
chitosan | fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), pH 5.6, 55°C | Renibacterium sp. QD1 | |
| 3.2.1.132 | 72000 | - |
chitosan | wild-type enzyme, pH 5.6, 55°C | Renibacterium sp. QD1 | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.132 | 5.6 | - |
wild-type enzyme, fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module BgCBM5 from Burkholderia gladioli (CsnA-CBM5), fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module PfCBM32-2 from Paenibacillus fukuinensis (CsnA-CBM32-2) and fusion protein of Renibacterium sp. QD1 chitosanase CsnA and the carbohydrate binding module AoCBM35 from Amycolatopsis orientalis (CsnA-CBM35) | Renibacterium sp. QD1 |
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