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Literature summary extracted from

  • Machado, C.B.; Citadini, A.P.; Goldbeck, R.; de Lima, E.A.; Figueiredo, F.L.; da Silva, T.M.; Hoffmam, Z.B.; de Sousa, A.S.; Squina, F.M.; de Lourdes Teixeira de Moraes Polizeli, M.; Ruller, R.; Ward, R.J.
    Increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from Bacillus licheniformis (2015), Biotechnol. Lett., 37, 1455-1462 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.99 additional information the hydrolysis of pretreated sugar cane biomass increases by 15% when a commercial enzyme cocktail is supplemented with immobilized ABNase. Pectin hydrolysis by recombinant ABNase plays a role in the effective application of enzymatic cocktails for biomass saccharification Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99
-
 Bacillus licheniformis

General Stability

EC Number General Stability Organism
3.2.1.99 the enzyme stability increases 128fold when immobilized with glyoxyl agarose Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.99 Cu2+ 5 mM, complete inhibition Bacillus licheniformis
3.2.1.99 EDTA 10 mM, 58% loss of activity Bacillus licheniformis
3.2.1.99 EGTA 10 mM, 30% loss of activity Bacillus licheniformis
3.2.1.99 Fe3+ 5 mM, complete inhibition Bacillus licheniformis
3.2.1.99 Zn2+ 5 mM, complete inhibition Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.99 CaCl2 5 mM, 1.1fold activation Bacillus licheniformis
3.2.1.99 KCl 5 mM, 1.1fold activation Bacillus licheniformis
3.2.1.99 LiCl 5 mM, 1.2fold activation Bacillus licheniformis
3.2.1.99 MgCl2 5 mM, 1.3fold activation Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 33000
-
-
 Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Bacillus licheniformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99
-
 Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 branched arabinan + H2O branched arabinan from sugar beet. Hydrolyzed at 12% compared to debranched arabinan Bacillus licheniformis ?
-
 ?
3.2.1.99 debranched arabinan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.99 linear arabinan + H2O hydrolyzed at 62% compared to debranched arabinan Bacillus licheniformis ?
-
 ?
3.2.1.99 additional information no activity with: laminarin, lichenan, galactomannan, beta-glucan, wheat and rye arabinoxylan, xyloglucan, carboxymethyl cellulose, pectin from fruits, larch arabinogalactan, galactan (potato), xylan from beechwood, and 1,4-beta-mannan Bacillus licheniformis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 ABNase
-
 Bacillus licheniformis
3.2.1.99 endo-arabinanase
-
 Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 35 40
-
 Bacillus licheniformis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.99 30 45 30°C: about 75% of maximal activity, 45°C: about 70% of maximal activity Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.99 50
-
 50% loss of activity after 30 min incubation at 50°C. After 60 min the activity is about 20% of the initial value Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 5.5 6.5
-
 Bacillus licheniformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.99 5 8.5 pH 5.0: about 65% of maximal activity, pH 8.5: about 40% of maximal activity Bacillus licheniformis