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Literature summary extracted from

  • Duan, X.; Liu, Y.; You, X.; Jiang, Z.; Yang, S.; Yang, S.
    High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production (2017), Biotechnol. Biofuels, 10, 223 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.74 SDS 5%, 131% of initial activity Malbranchea cinnamomea
3.1.1.74 Triton X-100 5%, 190% of initial activity Malbranchea cinnamomea
3.1.1.74 Tween 20 5%, 118% of initial activity Malbranchea cinnamomea
3.1.1.74 Tween 40 5%, 139.7% of initial activity Malbranchea cinnamomea
3.1.1.74 Tween 60 5%, 139.5% of initial activity Malbranchea cinnamomea
3.1.1.74 Tween 80 5%, 141.9% of initial activity Malbranchea cinnamomea

Application

EC Number Application Comment Organism
3.1.1.74 synthesis enzyme synthesizes butyl butanoate with a maximum esterification efficiency of 96.9% at 4 h Malbranchea cinnamomea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.74 expression in Pichia pastoris Malbranchea cinnamomea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.74 structure at 1.76 A resolution in space group P21221 Malbranchea cinnamomea

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.74 acetonitrile 30%, 93.1% residual activity Malbranchea cinnamomea
3.1.1.74 dimethyl sulfoxide 30%, 80.9% residual activity Malbranchea cinnamomea
3.1.1.74 Ethanol 30%, 72.8 % residual activity Malbranchea cinnamomea
3.1.1.74 hexane 30%, 97.7% residual activity Malbranchea cinnamomea
3.1.1.74 Methanol 30%, 80% residual activity Malbranchea cinnamomea

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Malbranchea cinnamomea A0A1S6YJF3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.74 1148
-
 pH 8.0, 50°C, substrate 4-nitrophenyl butanoate Malbranchea cinnamomea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl butanoate + H2O
-
 Malbranchea cinnamomea 4-nitrophenol + butanoate
-
 ?
3.1.1.74 4-nitrophenyl hexanoate + H2O
-
 Malbranchea cinnamomea 4-nitrophenol + hexanoate
-
 ?
3.1.1.74 4-nitrophenyl myristate + H2O
-
 Malbranchea cinnamomea 4-nitrophenol + myristate
-
 ?
3.1.1.74 butanol + butanoate
-
 Malbranchea cinnamomea butyl butanoate + H2O
-
 ?
3.1.1.74 cutin + H2O
-
 Malbranchea cinnamomea cutin monomers
-
 ?
3.1.1.74 poly(butylene succinaste) + H2O
-
 Malbranchea cinnamomea ?
-
 ?
3.1.1.74 poly(caprolactone) + H2O
-
 Malbranchea cinnamomea ?
-
 ?
3.1.1.74 tributyrin + H2O
-
 Malbranchea cinnamomea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.74 ? x * 21180, calculated from sequence Malbranchea cinnamomea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.74 45
-
-
 Malbranchea cinnamomea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.74 75
-
 stable up to Malbranchea cinnamomea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 8
-
-
 Malbranchea cinnamomea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.74 3 10.5
-
 Malbranchea cinnamomea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.74 Malbranchea cinnamomea calculated from sequence
-
 5.2