Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Furukawa, S.; Fujikawa, T.; Koga, D.; Ide, A.
    Purification and some properties of exo-type fucoidanases from Vibrio sp. N-5 (1992), Biosci. Biotechnol. Biochem., 56, 1829-1834 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.B47 Co2+ 2 mM, slight activation Vibrio sp.
3.2.1.B47 Mn2+ 2 mM, slight activation Vibrio sp.
3.2.1.B47 Sn2+ 2 mM, slight activation Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B47 AgNO3 2 mM, 2-10% inhibition Vibrio sp.
3.2.1.B47 FeCl3 2 mM, 70-80% loss of activity Vibrio sp.
3.2.1.B47 HgCl2 2 mM, about 95% loss of activity Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B47 39500
-
fucoidanase E-1, SDS-PAGE Vibrio sp.
3.2.1.B47 67500
-
fucoidanase E-3, gel filtration Vibrio sp.
3.2.1.B47 68000
-
fucoidanase E-2, SDS-PAGE Vibrio sp.
3.2.1.B47 68000
-
fucoidanase E-3, SDS-PAGE Vibrio sp.
3.2.1.B47 68500
-
fucoidanase E-2, gel filtration Vibrio sp.
3.2.1.B47 158000
-
fucoidanase E-1, gel filtration Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B47 Vibrio sp.
-
-
-
3.2.1.B47 Vibrio sp. N-5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B47
-
Vibrio sp.

Storage Stability

EC Number Storage Stability Organism
3.2.1.B47 0°C, pH 7.5, 50 mM Tris-HCl buffer, stable for at least 6 months Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B47 fucoidan + H2O the enzyme hydrolyzes gagome- and mozuku-fucoidans to the same extent, but not futomozuku-fucoidan Vibrio sp. ?
-
?
3.2.1.B47 fucoidan + H2O the enzyme hydrolyzes gagome- and mozuku-fucoidans to the same extent, but not futomozuku-fucoidan Vibrio sp. N-5 ?
-
?
3.2.1.B47 additional information no hydrolysis of 4-nitrophenyl-alpha-L-fucopyranoside Vibrio sp. ?
-
?
3.2.1.B47 additional information no hydrolysis of 4-nitrophenyl-alpha-L-fucopyranoside Vibrio sp. N-5 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.B47 monomer 1 * 68000, fucoidanase E-2, SDS-PAGE Vibrio sp.
3.2.1.B47 monomer 1 * 68000, fucoidanase E-3, SDS-PAGE Vibrio sp.
3.2.1.B47 tetramer 4 * 39500, fucoidanase E-1, SDS-PAGE Vibrio sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B47 exo-type fucoidanase
-
Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B47 6
-
fucoidanase E-1 Vibrio sp.
3.2.1.B47 6
-
fucoidanase E-2 Vibrio sp.
3.2.1.B47 7.5
-
fucoidanase E-3 Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B47 50
-
stable up to Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.B47 4 9 stable Vibrio sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.B47 Vibrio sp. fucoidanase E-2, chromatofocusing
-
5.75
3.2.1.B47 Vibrio sp. fucoidanase E-1, chromatofocusing
-
5.8
3.2.1.B47 Vibrio sp. fucoidanase E-3, chromatofocusing
-
7.65