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Literature summary extracted from
- The promiscuous phosphomonoestearase activity of Archaeoglobus fulgidus CopA, a thermophilic Cu+ transport ATPase (2016), Biochim. Biophys. Acta, 1858, 1471-1478 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.8 | Phospholipid | addition of phospholipids leads to a significant increase in CopA phosphomonoesterase turnover | Archaeoglobus fulgidus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.2.2.8 | 0.11 | - |
ATP | pH 6.1, 75°C | Archaeoglobus fulgidus | |
| 7.2.2.8 | 9 | - |
4-nitrophenyl phosphate | pH 6.1, 75°C | Archaeoglobus fulgidus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.8 | Mg2+ | essential activator for the hydrolysis of 4-nitrophenylphosphate | Archaeoglobus fulgidus | |
| 7.2.2.8 | additional information | no effect on the reaction with 4-nitrophenylphosphate: Mn2+, Co2+, Sr2+, Ba2+ | Archaeoglobus fulgidus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.2.8 | Archaeoglobus fulgidus | O29777 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.8 | 4-nitrophenyl phosphate + H2O + Cu+[side 1] | - |
Archaeoglobus fulgidus | 4-nitrophenol + phosphate + Cu+[side 2] | - |
? | |
| 7.2.2.8 | ATP + H2O + Cu+[side 1] | catalytic proficiency of CopA is slightly higher toward its natural substrate ATP than toward 4-nitrophenyl phosphate | Archaeoglobus fulgidus | ADP + phosphate + Cu+[side 2] | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.2.2.8 | 70 | 80 | hydrolysis of 4-nitrophenyl phosphate | Archaeoglobus fulgidus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.2.2.8 | 0.26 | - |
ATP | pH 6.1, 75°C | Archaeoglobus fulgidus | |
| 7.2.2.8 | 5.9 | - |
4-nitrophenyl phosphate | pH 6.1, 75°C | Archaeoglobus fulgidus | |
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