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Literature summary extracted from

  • Touchette, M.H.; Bommineni, G.R.; Delle Bovi, R.J.; Gadbery, J.E.; Nicora, C.D.; Shukla, A.K.; Kyle, J.E.; Metz, T.O.; Martin, D.W.; Sampson, N.S.; Miller, W.T.; Tonge, P.J.; Seeliger, J.C.
    Diacyltransferase activity and chain length specificity of Mycobacterium tuberculosis PapA5 in the synthesis of alkyl beta-diol lipids (2015), Biochemistry, 54, 5457-5468 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.282 expression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.282 A382F almost complete loss of activity Mycobacterium tuberculosis
2.3.1.282 A382M 35% of wild-type activity with substrate 1-octanol, 39% with 5R,7R-undecane-5,7-diol, 48% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis
2.3.1.282 G129L 13% of wild-type activity with substrate 1-octanol, 4% with 5R,7R-undecane-5,7-diol, 13% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis
2.3.1.282 Q19K 16% of wild-type activity with substrate 1-octanol, 11% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis
2.3.1.282 Q19R 68% of wild-type activity with substrate 1-octanol, 48% with 5R,7R-undecane-5,7-diol, 45% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis
2.3.1.282 S380M almost complete loss of activity Mycobacterium tuberculosis
2.3.1.282 V16C/G328C 8% of wild-type activity with with 5R,7R-undecane-5,7-diol, 3% with pentadecane-5,7-diol, respectively Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.282 Mycobacterium tuberculosis P9WIN5
-
-
2.3.1.282 Mycobacterium tuberculosis H37Rv P9WIN5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.282 phosphoprotein Ser/Thr kinases PknB and PknE modify PapA5 on three overlapping Thr residues and a fourth Thr is unique to PknE phosphorylation Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.282 219
-
 wild-type, substrate 1-octanol, pH 6.5, 22°C Mycobacterium tuberculosis
2.3.1.282 311
-
 wild-type, substrate 5R,7R-undecane-5,7-diol, pH 6.5, 22°C Mycobacterium tuberculosis
2.3.1.282 454
-
 wild-type, substrate 5R,7R-pentadecane-5,7-diol, pH 6.5, 22°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.282 2 palmitoyl-CoA + 5R,7R-pentadecane-5,7-diol
-
 Mycobacterium tuberculosis 5,7-dipalmitoyl 5R,7R-pentadecane-5,7-diol + 2 CoA
-
 ?
2.3.1.282 2 palmitoyl-CoA + 5R,7R-pentadecane-5,7-diol
-
 Mycobacterium tuberculosis H37Rv 5,7-dipalmitoyl 5R,7R-pentadecane-5,7-diol + 2 CoA
-
 ?
2.3.1.282 2 palmitoyl-CoA + 5R,7R-undecane-5,7-diol
-
 Mycobacterium tuberculosis 5,7-dipalmitoyl 5R,7R-undecane-5,7-diol + 2 CoA
-
 ?
2.3.1.282 2 palmitoyl-CoA + 5R,7R-undecane-5,7-diol
-
 Mycobacterium tuberculosis H37Rv 5,7-dipalmitoyl 5R,7R-undecane-5,7-diol + 2 CoA
-
 ?
2.3.1.282 palmitoyl-CoA + 1-octanol
-
 Mycobacterium tuberculosis palmitoyl octanol + 2 CoA
-
 ?
2.3.1.282 palmitoyl-CoA + 1-octanol
-
 Mycobacterium tuberculosis H37Rv palmitoyl octanol + 2 CoA
-
 ?

General Information

EC Number General Information Comment Organism
2.3.1.282 physiological function PapA5 is the terminal enzyme in dimycocerosate biosynthesis with dual esterification activity Mycobacterium tuberculosis