EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.2.14 | expressed in Escherichia coli strain K27 and BL21 Star (DE3) cells | Cuphea viscosissima |
3.1.2.14 | expressed in Escherichia coli strain K27 and BL21 Star (DE3) cells | Cocos nucifera |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.2.14 | C348A | the mutant of isoform FatB2 shows about 60% of wild type activity | Cuphea viscosissima |
3.1.2.14 | C348S | the mutant of isoform FatB2 shows about 140% of wild type activity | Cuphea viscosissima |
3.1.2.14 | C352A | the mutant of isoform FatB1 shows reduced activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | C352S | the mutant of isoform FatB1 shows reduced activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | C358A | the mutant shows increased activity compared to the wild type enzyme | Cocos nucifera |
3.1.2.14 | c358S | the mutant shows reduced activity compared to the wild type enzyme | Cocos nucifera |
3.1.2.14 | D309A | the mutant of isoform FatB2 has less than 3% activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | D309E | inactive | Cuphea viscosissima |
3.1.2.14 | D309N | the mutant of isoform FatB2 has less than 5% activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | E347A | inactive | Cuphea viscosissima |
3.1.2.14 | E347D | inactive | Cuphea viscosissima |
3.1.2.14 | E347Q | inactive | Cuphea viscosissima |
3.1.2.14 | E351A | the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | E351D | the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | E357A | the mutant shows strongly reduced activity compared to the wild type enzyme | Cocos nucifera |
3.1.2.14 | E357D | the mutant shows strongly reduced activity compared to the wild type enzyme | Cocos nucifera |
3.1.2.14 | H313A | inactive | Cuphea viscosissima |
3.1.2.14 | H317A | the mutant of isoform FatB1 shows almost no activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | H323A | the mutant shows almost no activity compared to the wild type enzyme | Cocos nucifera |
3.1.2.14 | N311A | the mutant of isoform FatB2 has less than 8% activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | N315A | the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme | Cuphea viscosissima |
3.1.2.14 | N321A | the mutant shows strongly reduced activity compared to the wild type enzyme | Cocos nucifera |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.14 | an oleoyl-[acyl-carrier protein] + H2O | Cuphea viscosissima | - |
an [acyl-carrier protein] + oleate | - |
? | |
3.1.2.14 | an oleoyl-[acyl-carrier protein] + H2O | Cocos nucifera | - |
an [acyl-carrier protein] + oleate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.14 | Cocos nucifera | G3ESU7 | - |
- |
3.1.2.14 | Cuphea viscosissima | G3ESU9 | - |
- |
3.1.2.14 | Cuphea viscosissima | G3ESV0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.14 | Ni-NTA column chromatography | Cuphea viscosissima |
3.1.2.14 | Ni-NTA column chromatography | Cocos nucifera |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.14 | an oleoyl-[acyl-carrier protein] + H2O | - |
Cuphea viscosissima | an [acyl-carrier protein] + oleate | - |
? | |
3.1.2.14 | an oleoyl-[acyl-carrier protein] + H2O | - |
Cocos nucifera | an [acyl-carrier protein] + oleate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.2.14 | acyl-ACP TE | - |
Cuphea viscosissima |
3.1.2.14 | acyl-ACP TE | - |
Cocos nucifera |
3.1.2.14 | acyl-ACP-thioesterase | - |
Cuphea viscosissima |
3.1.2.14 | acyl-ACP-thioesterase | - |
Cocos nucifera |
3.1.2.14 | acyl-acyl carrier protein thioesterase | - |
Cuphea viscosissima |
3.1.2.14 | acyl-acyl carrier protein thioesterase | - |
Cocos nucifera |
3.1.2.14 | FATB1 | isoform | Cuphea viscosissima |
3.1.2.14 | FatB2 | isoform | Cuphea viscosissima |
3.1.2.14 | FatB2 | isoform | Cocos nucifera |