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Literature summary extracted from

  • Jing, F.; Yandeau-Nelson, M.D.; Nikolau, B.J.
    Identification of active site residues implies a two-step catalytic mechanism for acyl-ACP thioesterase (2018), Biochem. J., 475, 3861-3873 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 expressed in Escherichia coli strain K27 and BL21 Star (DE3) cells Cuphea viscosissima
3.1.2.14 expressed in Escherichia coli strain K27 and BL21 Star (DE3) cells Cocos nucifera

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.14 C348A the mutant of isoform FatB2 shows about 60% of wild type activity Cuphea viscosissima
3.1.2.14 C348S the mutant of isoform FatB2 shows about 140% of wild type activity Cuphea viscosissima
3.1.2.14 C352A the mutant of isoform FatB1 shows reduced activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 C352S the mutant of isoform FatB1 shows reduced activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 C358A the mutant shows increased activity compared to the wild type enzyme Cocos nucifera
3.1.2.14 c358S the mutant shows reduced activity compared to the wild type enzyme Cocos nucifera
3.1.2.14 D309A the mutant of isoform FatB2 has less than 3% activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 D309E inactive Cuphea viscosissima
3.1.2.14 D309N the mutant of isoform FatB2 has less than 5% activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 E347A inactive Cuphea viscosissima
3.1.2.14 E347D inactive Cuphea viscosissima
3.1.2.14 E347Q inactive Cuphea viscosissima
3.1.2.14 E351A the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 E351D the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 E357A the mutant shows strongly reduced activity compared to the wild type enzyme Cocos nucifera
3.1.2.14 E357D the mutant shows strongly reduced activity compared to the wild type enzyme Cocos nucifera
3.1.2.14 H313A inactive Cuphea viscosissima
3.1.2.14 H317A the mutant of isoform FatB1 shows almost no activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 H323A the mutant shows almost no activity compared to the wild type enzyme Cocos nucifera
3.1.2.14 N311A the mutant of isoform FatB2 has less than 8% activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 N315A the mutant of isoform FatB1 shows strongly reduced activity compared to the wild type enzyme Cuphea viscosissima
3.1.2.14 N321A the mutant shows strongly reduced activity compared to the wild type enzyme Cocos nucifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.14 an oleoyl-[acyl-carrier protein] + H2O Cuphea viscosissima
-
an [acyl-carrier protein] + oleate
-
?
3.1.2.14 an oleoyl-[acyl-carrier protein] + H2O Cocos nucifera
-
an [acyl-carrier protein] + oleate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Cocos nucifera G3ESU7
-
-
3.1.2.14 Cuphea viscosissima G3ESU9
-
-
3.1.2.14 Cuphea viscosissima G3ESV0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.14 Ni-NTA column chromatography Cuphea viscosissima
3.1.2.14 Ni-NTA column chromatography Cocos nucifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 an oleoyl-[acyl-carrier protein] + H2O
-
Cuphea viscosissima an [acyl-carrier protein] + oleate
-
?
3.1.2.14 an oleoyl-[acyl-carrier protein] + H2O
-
Cocos nucifera an [acyl-carrier protein] + oleate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 acyl-ACP TE
-
Cuphea viscosissima
3.1.2.14 acyl-ACP TE
-
Cocos nucifera
3.1.2.14 acyl-ACP-thioesterase
-
Cuphea viscosissima
3.1.2.14 acyl-ACP-thioesterase
-
Cocos nucifera
3.1.2.14 acyl-acyl carrier protein thioesterase
-
Cuphea viscosissima
3.1.2.14 acyl-acyl carrier protein thioesterase
-
Cocos nucifera
3.1.2.14 FATB1 isoform Cuphea viscosissima
3.1.2.14 FatB2 isoform Cuphea viscosissima
3.1.2.14 FatB2 isoform Cocos nucifera