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Literature summary extracted from
- Characterization of human ATP-binding cassette protein subfamily D reconstituted into proteoliposomes (2018), Biochem. Biophys. Res. Commun., 496, 1122-1127 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.6.2.4 | gene ABCD1, recombinant expression of His-tagged protein ABCD1 in Pichia pastoris strain SMD1168 | Homo sapiens |
| 7.6.2.4 | gene ABCD2, recombinant expression of protein ABCD2 in Pichia pastoris strain SMD1168 | Homo sapiens |
| 7.6.2.4 | gene ABCD3, recombinant expression of protein ABCD3 in Pichia pastoris strain SMD1168 | Homo sapiens |
| 7.6.2.8 | gene ABCD4, recombinant expression of His-tagged protein ABCD4 in Pichia pastoris strain SMD1168 | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.4 | AlF3 | moderate inhibition of the ATPase activity of liposome-reconstituted His-hABCD3; strong inhibition of the ATPase activity of liposome-reconstituted His-hABCD1; weak inhibition of the ATPase activity of liposome-reconstituted His-hABCD2 | Homo sapiens |  |
| 7.6.2.4 | additional information | no inhibition of the ATPase activity of liposome-reconstituted His-hABCD1 by NaN3, EGTA or ouabain | Homo sapiens | |
| 7.6.2.8 | AlF3 | inhibition of the ATPase activity of liposome-reconstituted His-hABCD4 | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.6.2.4 | peroxisomal membrane | - |
Homo sapiens | 5778 | - |
| 7.6.2.8 | lysosomal membrane | ABCD4 dimer makes a complex with lysosomal membrane protein LMBD1, an adopter protein, and targets to lysosome | Homo sapiens | 5765 | - |
| 7.6.2.8 | additional information | ABCD4 barely interacts with the peroxisomal biogenesis factor Pex19p, because of its lack of NH2-terminal hydrophobic motif. As a result, the transmembrane domain (TMD) 1 of ABCD4 is recognized by certain signal recognition particles and integrated into the ER membrane. ABCD4 dimer then makes a complex with lysosomal membrane protein LMBD1, an adopter protein, and targets to lysosome | Homo sapiens | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.4 | Mg2+ | required | Homo sapiens | |
| 7.6.2.8 | Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.4 | ATP + H2O + fatty acyl CoA[side 1] | Homo sapiens | - |
ADP + phosphate + fatty acyl CoA[side 2] | - |
? | |
| 7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | Homo sapiens | - |
ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.6.2.4 | Homo sapiens | P28288 | - |
- |
| 7.6.2.4 | Homo sapiens | P33897 | - |
- |
| 7.6.2.4 | Homo sapiens | Q9UBJ2 | - |
- |
| 7.6.2.8 | Homo sapiens | O14678 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.6.2.4 | recombinant His-tagged protein ABCD1 from Pichia pastoris strain SMD1168 by detergent solubilization and nickel affinity chromatography, evaluation of diverse detergents | Homo sapiens |
| 7.6.2.4 | recombinant His-tagged protein ABCD2 from Pichia pastoris strain SMD1168 by detergent solubilization and nickel affinity chromatography, evaluation of diverse detergents | Homo sapiens |
| 7.6.2.4 | recombinant His-tagged protein ABCD3 from Pichia pastoris strain SMD1168 by detergent solubilization and nickel affinity chromatography, evaluation of diverse detergents | Homo sapiens |
| 7.6.2.8 | recombinant His-tagged protein ABCD4 from Pichia pastoris strain SMD1168 by detergent solubilization and nickel affinity chromatography, evaluation of diverse detergents | Homo sapiens |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 7.6.2.4 | recombinant His-tagged protein ABCD1 protein solubilization and reconstitution in proteoliposomes | Homo sapiens |
| 7.6.2.4 | recombinant His-tagged protein ABCD2 protein solubilization and reconstitution in proteoliposomes, the liposome-reconstituted His-hABCD1 shows ATPase activity | Homo sapiens |
| 7.6.2.4 | recombinant His-tagged protein ABCD3 protein solubilization and reconstitution in proteoliposomes | Homo sapiens |
| 7.6.2.8 | recombinant His-tagged protein ABCD4 protein solubilization and reconstitution in proteoliposomes | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.4 | ATP + H2O + fatty acyl CoA[side 1] | - |
Homo sapiens | ADP + phosphate + fatty acyl CoA[side 2] | - |
? | |
| 7.6.2.4 | ATP + H2O + NBD-palmitoyl-CoA[side 1] | - |
Homo sapiens | ADP + phosphate + NBD-palmitoyl-CoA[side 2] | - |
? | |
| 7.6.2.4 | additional information | activity measurement by malachite green phosphate determination assay. Ellman's reagent, 5,5-dithiobis(2-nitrobenzoic acid), is not sensitive enough to measure the activity of hABCD1. The enzyme possesses an equal levels of acyl-CoA thioesterase activity | Homo sapiens | ? | - |
? | |
| 7.6.2.4 | additional information | activity measurement by malachite green phosphate determination assay. Enzyme hABCD2 possesses acyl-CoA thioesterase activity | Homo sapiens | ? | - |
? | |
| 7.6.2.4 | additional information | activity measurement by malachite green phosphate determination assay. Enzyme hABCD3 possesses acyl-CoA thioesterase activity | Homo sapiens | ? | - |
? | |
| 7.6.2.8 | ATP + H2O + vitamin B12-[cobalamin-binding protein][side 1] | - |
Homo sapiens | ADP + phosphate + vitamin B12[side 2] + [cobalamin-binding protein][side 1] | - |
? | |
| 7.6.2.8 | additional information | the enzyme possesses equal levels of acyl-CoA thioesterase activity, ATPase and thioesterase activities of liposome-reconstituted human ABCD4, overview | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.6.2.4 | ? | x * 70000, recombinant His-tagged ABCD3 | Homo sapiens |
| 7.6.2.4 | ? | x * 75000, recombinant His-tagged ABCD1 | Homo sapiens |
| 7.6.2.4 | ? | x * 75000, recombinant His-tagged ABCD2 | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.6.2.4 | ABCD1 | - |
Homo sapiens |
| 7.6.2.4 | ABCD2 | - |
Homo sapiens |
| 7.6.2.4 | ABCD3 | - |
Homo sapiens |
| 7.6.2.8 | ABCD4 | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.4 | 37 | - |
assay at | Homo sapiens |
| 7.6.2.8 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.4 | 7.6 | - |
assay at | Homo sapiens |
| 7.6.2.8 | 7.6 | - |
assay at | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.4 | ATP | - |
Homo sapiens | |
| 7.6.2.8 | ATP | - |
Homo sapiens | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.6.2.4 | evolution | ABCD1-3 belong to the ATP-binding cassette protein superfamily, subfamily D, membrane-bound proteins with highly conserved structure | Homo sapiens |
| 7.6.2.8 | malfunction | ABCD4 dysfunction results in a failure of lysosomal vitamin B12 release | Homo sapiens |
| 7.6.2.8 | physiological function | ABCD4 is located on lysosomal membrane and is involved in the transport of vitamin B12 from lysosomes to the cytosol | Homo sapiens |
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Release 2023.1 (January 2023)
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