EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.130 | - |
Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.1.130 | LysK in complex with lysine at a resolution of 2.4 A. The alpha-amino group of the bound lysine is oriented toward the catalytic center, which is composed of the residues coordinating divalent metal ions for the hydrolysis of the isopeptide bond. An 11 A long path leads from the active site binding lysine to the protein surface. A positively-charged surface region is located around the exit of the path | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.130 | D325A | marked decrease in activity | Thermus thermophilus |
3.5.1.130 | K297E | marked decrease in activity | Thermus thermophilus |
3.5.1.130 | R282E | marked decrease in activity | Thermus thermophilus |
3.5.1.130 | R286E | marked decrease in activity | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.130 | Thermus thermophilus | Q8VUS5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.130 | [carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O | - |
Thermus thermophilus | [carrier protein LysW]-C-terminal-L-glutamate + L-lysine | - |
? | |
3.5.1.130 | [CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O | i.e. the C-terminal 10 residues of LysW fused with one lysine residue at its gamma-carboxyl group of C-terminal Glu54 | Thermus thermophilus | [CT10-LysW]-C-terminal-L-glutamate + L-lysine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.130 | lysK | - |
Thermus thermophilus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.130 | 3.1 | - |
[CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate | mutant R286E, pH 8.0, 60°C | Thermus thermophilus | |
3.5.1.130 | 4.5 | - |
[CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate | mutant R282E, pH 8.0, 60°C | Thermus thermophilus | |
3.5.1.130 | 5.5 | - |
[CT10-LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate | wild-type, pH 8.0, 60°C | Thermus thermophilus | |
3.5.1.130 | 36 | - |
[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate | mutant R286E, pH 8.0, 60°C | Thermus thermophilus | |
3.5.1.130 | 38 | - |
[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate | mutant R282E, pH 8.0, 60°C | Thermus thermophilus | |
3.5.1.130 | 530 | - |
[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate | wild-type, pH 8.0, 60°C | Thermus thermophilus |