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Literature summary extracted from

  • Sanchez-Romero, J.J.; Olguin, L.F.
    Choline sulfatase from Ensifer (Sinorhizobium) meliloti Characterization of the unmodified enzyme (2015), Biochem. Biophys. Rep., 3, 161-168 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.6 expression in Escherichia coli Sinorhizobium meliloti

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.6 choline competitive with respect to choline sulfate Sinorhizobium meliloti
3.1.6.6 Choline sulfate substrate inhibition Sinorhizobium meliloti
3.1.6.6 additional information not inhibitory: ammonium sulfate up to 70 mM Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.6 11.1
-
 Choline sulfate pH 7.5, 25°C Sinorhizobium meliloti
3.1.6.6 11.8
-
 4-Methylumbelliferyl sulfate pH 7.5, 25°C Sinorhizobium meliloti
3.1.6.6 75.8
-
 4-nitrophenyl sulfate pH 7.5, 25°C Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.6 118000
-
 MALDI-TOF Sinorhizobium meliloti
3.1.6.6 123000
-
 gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.6 Sinorhizobium meliloti O69787
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.6.6 additional information absence of formylglycine posttranslational modification in active-site cysteine54 Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.6 4-methylumbelliferyl sulfate + H2O
-
 Sinorhizobium meliloti methylumbelliferone + sulfate
-
 ?
3.1.6.6 4-nitrophenyl sulfate + H2O
-
 Sinorhizobium meliloti 4-nitrophenol + sulfate
-
 ?
3.1.6.6 choline sulfate + H2O
-
 Sinorhizobium meliloti choline + sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.6.6 dimer 2 * 63000, SDS-PAGE, recombinant protein including His-tag, 2 * 59000, MALDI-TOF Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.6.6 0.035
-
 4-nitrophenyl sulfate pH 7.5, 25°C Sinorhizobium meliloti
3.1.6.6 0.043
-
 4-Methylumbelliferyl sulfate pH 7.5, 25°C Sinorhizobium meliloti
3.1.6.6 0.27
-
 Choline sulfate pH 7.5, 25°C Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.6 6.5
-
-
 Sinorhizobium meliloti

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.6.6 11.4
-
 Choline sulfate pH 7.5, 25°C Sinorhizobium meliloti
3.1.6.6 57.8
-
 choline pH 7.5, 25°C Sinorhizobium meliloti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.6.6 0.00046
-
 4-nitrophenyl sulfate pH 7.5, 25°C Sinorhizobium meliloti
3.1.6.6 0.0036
-
 4-Methylumbelliferyl sulfate pH 7.5, 25°C Sinorhizobium meliloti
3.1.6.6 0.0243
-
 Choline sulfate pH 7.5, 25°C Sinorhizobium meliloti