Literature summary extracted from

Bonfa, E.; de Souza Moretti, M.; Gomes, E.; Bonilla-Rodriguez, G.
Biochemical characterization of an isolated 50kDa beta-glucosidase from the thermophilic fungus Myceliophthora thermophila M.7.7 (2018), Biocatal. Agricult. Biotechnol., 13, 311-318 .

No PubMed abstract available

Activating Compound

EC Number	Activating Compound	Comment	Organism
3.2.1.21	4-hydroxybenzoic acid	109.7% activity at 2.5 mM with cellobioside as substrate	Thermothelomyces thermophilus
3.2.1.21	p-coumaric acid	118% activity at 2.5 mM with cellobioside as substrate	Thermothelomyces thermophilus
3.2.1.21	syringic acid	111.4% activity at 2.5 mM with cellobioside as substrate	Thermothelomyces thermophilus
3.2.1.21	vanillic acid	125.1% activity at 2.5 mM with cellobioside as substrate	Thermothelomyces thermophilus
3.2.1.21	Vanillin	118% activity at 2.5 mM with cellobioside as substrate	Thermothelomyces thermophilus

Inhibitors

EC Number	Inhibitors	Comment	Organism
3.2.1.21	4-hydroxybenzoic acid	97.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate	Thermothelomyces thermophilus
3.2.1.21	acetone	68.3% and 62% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	Al3+	83.5% and 43.7% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	Ba2+	76.7% residual activity at 2.5 mM with cellobiose as substrate	Thermothelomyces thermophilus
3.2.1.21	Cu2+	complete inhibition at 2.5 mM	Thermothelomyces thermophilus
3.2.1.21	D-glucose	competitive inhibition	Thermothelomyces thermophilus
3.2.1.21	dithiothreitol	82.5% residual activity and complete inhibition at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	DMSO	92.9% and 57% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	ethanol	70.6% and 55.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	Fe2+	complete inhibition at 2.5 mM	Thermothelomyces thermophilus
3.2.1.21	ferulic acid	97.4% and 99.1% residual activity at 0.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	gallic acid	91.1% and 75.3% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	Isopropanol	66.5-70.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	K+	76.2% and 81.9% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	Mg2+	62.7% residual activity at 2.5 mM with cellobiose as substrate	Thermothelomyces thermophilus
3.2.1.21	Mn2+	49.6% and 67.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	Na+	70.6% and 57.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	p-coumaric acid	70.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate	Thermothelomyces thermophilus
3.2.1.21	PEG 8000	67.6% and 58.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	polyvinyl alcohol	71.2% and 56.8% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	SDS	82.4% and 60.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	syringaldehyde	92.8% and 96.4% residual activity at 0.25 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	syringic acid	95.2% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate	Thermothelomyces thermophilus
3.2.1.21	Tannic acid	complete inhibition at 0.0625 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate	Thermothelomyces thermophilus
3.2.1.21	Triton X-100	57.5% and 45% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus
3.2.1.21	vanillic acid	87.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate	Thermothelomyces thermophilus
3.2.1.21	Vanillin	94% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate	Thermothelomyces thermophilus
3.2.1.21	Zn2+	63% residual activity and complete inhibition at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively	Thermothelomyces thermophilus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.1.21	0.52	-	4-nitrophenyl beta-D-glucopyranoside	at pH 5.0 and 60°C	Thermothelomyces thermophilus
3.2.1.21	8.5	-	cellobiose	at pH 5.0 and 50°C	Thermothelomyces thermophilus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.2.1.21	Li+	102.6-128.4% activity at 2.5 mM	Thermothelomyces thermophilus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.21	Thermothelomyces thermophilus	-	-	-
3.2.1.21	Thermothelomyces thermophilus M.7.7	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.21	Q-Sepharose resin column chromatography and Sephacrly S-100 gel filtration	Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3.2.1.21	4-nitrophenyl beta-D-glucopyranoside + H2O	-	Thermothelomyces thermophilus	4-nitrophenol + beta-D-glucopyranose	-	?
3.2.1.21	4-nitrophenyl beta-D-glucopyranoside + H2O	-	Thermothelomyces thermophilus M.7.7	4-nitrophenol + beta-D-glucopyranose	-	?
3.2.1.21	cellobiose + H2O	-	Thermothelomyces thermophilus	2 beta-D-glucose	-	?
3.2.1.21	cellobiose + H2O	-	Thermothelomyces thermophilus M.7.7	2 beta-D-glucose	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.2.1.21	?	x * 50000, SDS-PAGE	Thermothelomyces thermophilus

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.21	BGL50	-	Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.2.1.21	50	-	with cellobiose as substrate	Thermothelomyces thermophilus
3.2.1.21	60	-	with 4-nitrophenyl beta-D-glucopyranoside as substrate	Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.2.1.21	60	-	the enzyme is very stable at 60°C displaying a half-life of 855.6 min but it is easily denatured above this temperature	Thermothelomyces thermophilus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.2.1.21	4.5	-	with cellobiose as substrate	Thermothelomyces thermophilus
3.2.1.21	5	-	with 4-nitrophenyl beta-D-glucopyranoside as substrate	Thermothelomyces thermophilus

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
3.2.1.21	1.5	-	D-glucose	at pH 5.0 and 60°C	Thermothelomyces thermophilus