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Literature summary extracted from
- Characterization of butyrylcholinesterase from porcine milk (2018), Arch. Biochem. Biophys., 652, 38-49 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.8 | acetylthiocholine | - |
Homo sapiens |  |
| 3.1.1.8 | butyrylthiocholine | - |
Homo sapiens | |
| 3.1.1.8 | additional information | enzyme HuBChE shows marked substrate activation with butyrylthiocholine, acetylthiocholine, and propionylthiocholine | Homo sapiens | |
| 3.1.1.8 | additional information | enzyme PoBChE does not show substrate activation with acetylthiocholine, propionylthiocholine, and butyrylthiocholine | Sus scrofa | |
| 3.1.1.8 | propionylthiocholine | - |
Homo sapiens | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.7 | recombinant expression of HuAChE in CHO cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.7 | (-)-huperzine A | - |
Homo sapiens | |
| 3.1.1.7 | 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | i.e. BW284c51 | Homo sapiens | |
| 3.1.1.7 | acetylthiocholine | - |
Homo sapiens | |
| 3.1.1.7 | butyrylthiocholine | - |
Homo sapiens | |
| 3.1.1.7 | decamethonium | - |
Homo sapiens | |
| 3.1.1.7 | diisopropyl fluorophosphate | inhibition, aging, and reactivation kinetics | Homo sapiens | |
| 3.1.1.7 | edrophonium | - |
Homo sapiens | |
| 3.1.1.7 | ethopropazine | - |
Homo sapiens | |
| 3.1.1.7 | additional information | recombinant HuAChE shows substrate inhibition | Homo sapiens | |
| 3.1.1.7 | phenothiazine | - |
Homo sapiens | |
| 3.1.1.7 | propidium | - |
Homo sapiens | |
| 3.1.1.7 | propionylthiocholine | - |
Homo sapiens | |
| 3.1.1.7 | tacrine | - |
Homo sapiens | |
| 3.1.1.7 | tetra(monoisopropyl)diphosphortetramide | irreversible | Homo sapiens | |
| 3.1.1.8 | (-)-huperzine A | - |
Homo sapiens | |
| 3.1.1.8 | (-)-huperzine A | - |
Sus scrofa | |
| 3.1.1.8 | 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | i.e. BW284c51 | Homo sapiens | |
| 3.1.1.8 | 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | i.e. BW284c51 | Sus scrofa | |
| 3.1.1.8 | acetylthiocholine | slight substrate inhibition | Sus scrofa | |
| 3.1.1.8 | butyrylthiocholine | slight substrate inhibition | Sus scrofa | |
| 3.1.1.8 | decamethonium | - |
Homo sapiens | |
| 3.1.1.8 | decamethonium | - |
Sus scrofa | |
| 3.1.1.8 | diisopropyl fluorophosphate | inhibition, aging, and reactivation kinetics | Homo sapiens | |
| 3.1.1.8 | diisopropyl fluorophosphate | inhibition, aging, and reactivation kinetics | Sus scrofa | |
| 3.1.1.8 | edrophonium | - |
Homo sapiens | |
| 3.1.1.8 | edrophonium | - |
Sus scrofa | |
| 3.1.1.8 | ethopropazine | - |
Homo sapiens | |
| 3.1.1.8 | ethopropazine | - |
Sus scrofa | |
| 3.1.1.8 | additional information | PoBChE is sensitive to BChE-specific inhibitors, but shows only slight substrate inhibition | Sus scrofa | |
| 3.1.1.8 | phenothiazine | - |
Homo sapiens | |
| 3.1.1.8 | phenothiazine | - |
Sus scrofa | |
| 3.1.1.8 | propidium | - |
Homo sapiens | |
| 3.1.1.8 | propidium | - |
Sus scrofa | |
| 3.1.1.8 | propionylthiocholine | slight substrate inhibition | Sus scrofa | |
| 3.1.1.8 | tacrine | - |
Homo sapiens | |
| 3.1.1.8 | tacrine | - |
Sus scrofa | |
| 3.1.1.8 | tetra(monoisopropyl)diphosphortetramide | irreversible | Homo sapiens | |
| 3.1.1.8 | tetra(monoisopropyl)diphosphortetramide | irreversible | Sus scrofa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.7 | 0.11 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 0.15 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.015 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.017 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.031 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.23 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.25 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.37 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.8 | 340000 | - |
native PAGE | Sus scrofa |
| 3.1.1.8 | 342000 | 353000 | analytical ultracentrifugation | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.7 | acetylcholine + H2O | Homo sapiens | - |
choline + acetate | - |
? | |
| 3.1.1.8 | butyrylcholine + H2O | Homo sapiens | - |
choline + butyrate | - |
? | |
| 3.1.1.8 | butyrylcholine + H2O | Sus scrofa | - |
choline + butyrate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.7 | Homo sapiens | P22303 | - |
- |
| 3.1.1.8 | Homo sapiens | P06276 | - |
- |
| 3.1.1.8 | Sus scrofa | P32752 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.7 | recombinant enzyme HuAChE from CHO cells by procainamide affinity chromatography | Homo sapiens |
| 3.1.1.8 | native enzyme BChE from defatted milk by procainamide affinity chromatography and ultrafiltration, reduced and denatured PoBChE purified by hupresin affinity chromatography | Sus scrofa |
| 3.1.1.8 | native enzyme is purified from Cohn fraction IV-4 paste | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.8 | milk | - |
Homo sapiens | - |
| 3.1.1.8 | milk | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.8 | 360 | 650 | purified native enzyme from milk, pH 8.0, 22°C | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.7 | acetylcholine + H2O | - |
Homo sapiens | choline + acetate | - |
? | |
| 3.1.1.7 | acetylthiocholine + H2O | - |
Homo sapiens | thiocholine + acetate | - |
? | |
| 3.1.1.7 | additional information | no activity with butyrylcholine | Homo sapiens | ? | - |
? | |
| 3.1.1.7 | propionylthiocholine + H2O | - |
Homo sapiens | thiocholine + propionate | - |
? | |
| 3.1.1.8 | acetylthiocholine + H2O | - |
Homo sapiens | thiocholine + acetate | - |
? | |
| 3.1.1.8 | acetylthiocholine + H2O | - |
Sus scrofa | thiocholine + acetate | - |
? | |
| 3.1.1.8 | butyrylcholine + H2O | - |
Homo sapiens | choline + butyrate | - |
? | |
| 3.1.1.8 | butyrylcholine + H2O | - |
Sus scrofa | choline + butyrate | - |
? | |
| 3.1.1.8 | butyrylthiocholine + H2O | - |
Homo sapiens | thiocholine + butyrate | - |
? | |
| 3.1.1.8 | butyrylthiocholine + H2O | - |
Sus scrofa | thiocholine + butyrate | - |
? | |
| 3.1.1.8 | additional information | PoBChE hydrolyzes acetylthiocholine slightly faster than butyrylthiocholine | Sus scrofa | ? | - |
? | |
| 3.1.1.8 | propionylthiocholine + H2O | - |
Homo sapiens | thiocholine + propionate | - |
? | |
| 3.1.1.8 | propionylthiocholine + H2O | - |
Sus scrofa | thiocholine + propionate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.8 | tetramer | 4 * 85000, SDS-PAGE | Sus scrofa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.7 | AChE | - |
Homo sapiens |
| 3.1.1.7 | HuAChE | - |
Homo sapiens |
| 3.1.1.8 | BChE | - |
Homo sapiens |
| 3.1.1.8 | BChE | - |
Sus scrofa |
| 3.1.1.8 | butyrylcholinesterase | - |
Homo sapiens |
| 3.1.1.8 | butyrylcholinesterase | - |
Sus scrofa |
| 3.1.1.8 | HuBChE | - |
Homo sapiens |
| 3.1.1.8 | PoBChE | - |
Sus scrofa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.7 | 22 | - |
assay at | Homo sapiens |
| 3.1.1.8 | 22 | - |
assay at | Homo sapiens |
| 3.1.1.8 | 22 | - |
assay at | Sus scrofa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.7 | 3.72 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 7.18 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 4.78 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 5.6 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 38.3 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 50 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 53.3 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.7 | 8 | - |
assay at | Homo sapiens |
| 3.1.1.8 | 8 | - |
assay at | Homo sapiens |
| 3.1.1.8 | 8 | - |
assay at | Sus scrofa |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.7 | 0.00000032 | - |
(-)-huperzine A | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 0.000003 | - |
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 0.000037 | - |
tacrine | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 0.00036 | - |
edrophonium | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 0.00049 | - |
propidium | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 0.0022 | - |
decamethonium | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 0.0046 | - |
tetra(monoisopropyl)diphosphortetramide | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 0.0653 | - |
ethopropazine | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.00000043 | - |
tacrine | pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.000008 | - |
tacrine | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.00005 | - |
tetra(monoisopropyl)diphosphortetramide | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.000051 | - |
ethopropazine | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.00006 | - |
tetra(monoisopropyl)diphosphortetramide | pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.00019 | - |
ethopropazine | pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.0004 | - |
propidium | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.0008 | - |
decamethonium | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.0019 | - |
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.0093 | - |
1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide | pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.0117 | - |
propidium | pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.0185 | - |
edrophonium | pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.0286 | - |
(-)-huperzine A | pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 0.0504 | - |
edrophonium | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.0756 | - |
(-)-huperzine A | pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 0.0875 | - |
decamethonium | pH 8.0, 22°C | Sus scrofa | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.7 | additional information | the concentration of active sites in rHuAChE is determined by titration with 7-(O,O-diethyl-phosphinyloxy)-1-methylquinolinium methylsulfate (DEPQ) | Homo sapiens |
| 3.1.1.8 | additional information | the concentration of active sites in PoBChE is determined by titration with 7-(O,O-diethyl-phosphinyloxy)-1-methylquinolinium methylsulfate (DEPQ) | Sus scrofa |
| 3.1.1.8 | physiological function | PoBChE hydrolyzes acetylthiocholine slightly faster than butyrylthiocholine, but is sensitive to BChE-specific inhibitors | Sus scrofa |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.7 | 24.8 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.7 | 65.3 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 15.14 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 20.78 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Sus scrofa | |
| 3.1.1.8 | 1235.5 | - |
acetylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 3135.3 | - |
propionylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
| 3.1.1.8 | 3333.3 | - |
butyrylthiocholine | recombinant enzyme, pH 8.0, 22°C | Homo sapiens | |
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