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Literature summary extracted from

  • Kamon, M.; Sumitani, J.; Tani, S.; Kawaguchi, T.; Kamon, M.; Sumitani, J.; Tani, S.; Kawaguchi, T.
    Characterization and gene cloning of a maltotriose-forming exo-amylase from Kitasatospora sp. MK-1785 (2015), Appl. Microbiol. Biotechnol., 99, 4743-4753 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.116 expressed in Escherichia coli Kitasatospora sp. MK-1785

General Stability

EC Number General Stability Organism
3.2.1.116 the enzyme is subjected to proteolysis at N-terminal region of the anterior starch-binding domain CBM20 in the C-terminal region Kitasatospora sp. MK-1785

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.116 Ba2+
-
 Kitasatospora sp. MK-1785
3.2.1.116 Co2+
-
 Kitasatospora sp. MK-1785
3.2.1.116 Cu2+
-
 Kitasatospora sp. MK-1785
3.2.1.116 EDTA
-
 Kitasatospora sp. MK-1785
3.2.1.116 Mn2+
-
 Kitasatospora sp. MK-1785
3.2.1.116 Ni2+
-
 Kitasatospora sp. MK-1785
3.2.1.116 Zn2+
-
 Kitasatospora sp. MK-1785

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.116 23.8
-
 maltotetraose pH 6.0, 37°C Kitasatospora sp. MK-1785
3.2.1.116 33.9
-
 amylose pH 6.0, 37°C Kitasatospora sp. MK-1785
3.2.1.116 37.9
-
 maltopentaose pH 6.0, 37°C Kitasatospora sp. MK-1785

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.116 Ca2+ slightly enhances activity Kitasatospora sp. MK-1785
3.2.1.116 Li+ slightly enhances activity Kitasatospora sp. MK-1785
3.2.1.116 Mg2+ slightly enhances activity Kitasatospora sp. MK-1785
3.2.1.116 Sn2+ slightly enhances activity Kitasatospora sp. MK-1785

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.116 57000
-
 SDS-PAGE Kitasatospora sp. MK-1785

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.116 Kitasatospora sp. MK-1785 HW429890
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.116
-
 Kitasatospora sp. MK-1785

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.116 amylopectin + H2O 96.7% of the activity as compared to soluble starch Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?
3.2.1.116 amylose + H2O 89.6% of the activity as compared to soluble starch Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?
3.2.1.116 gamma-cyclodextrin + H2O 30.0% of the activity as compared to soluble starch Kitasatospora sp. MK-1785 maltotriose + maltose
-
 ?
3.2.1.116 glycogen + H2O 79.4% of the activity as compared to soluble starch Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?
3.2.1.116 maltohexaose + H2O
-
 Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?
3.2.1.116 maltopentaose + H2O
-
 Kitasatospora sp. MK-1785 maltotriose + maltose
-
 ?
3.2.1.116 maltotetraose + H2O
-
 Kitasatospora sp. MK-1785 maltotriose + glucose
-
 ?
3.2.1.116 additional information the enzyme exhibits transglycosylation activity toward phenolic and alcoholic compounds. It can not degrade pullulan Kitasatospora sp. MK-1785 ?
-
 ?
3.2.1.116 soluble starch + H2O the enzyme produces maltotriose as the principal product from starch Kitasatospora sp. MK-1785 maltotriose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.116 ? x * 57000, SDS-PAGE Kitasatospora sp. MK-1785

Synonyms

EC Number Synonyms Comment Organism
3.2.1.116 G3Amy
-
 Kitasatospora sp. MK-1785
3.2.1.116 maltotriose-forming exo-amylase
-
 Kitasatospora sp. MK-1785

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.116 55
-
-
 Kitasatospora sp. MK-1785

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.116 40 70 40°C: about 50% of maximal activity, 70°C: about 40% of maximal activity Kitasatospora sp. MK-1785

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.116 55
-
 the enzyme retains more than 80% activity after incubation for 20 min up to 50°C in the presence of 1 mM Ca2+ Kitasatospora sp. MK-1785

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.116 14.7
-
 maltotetraose pH 6.0, 37°C Kitasatospora sp. MK-1785
3.2.1.116 53.8
-
 amylose pH 6.0, 37°C Kitasatospora sp. MK-1785
3.2.1.116 58.1
-
 maltopentaose pH 6.0, 37°C Kitasatospora sp. MK-1785

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.116 6.5
-
-
 Kitasatospora sp. MK-1785

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.116 4 8 the enzyme retains more than 80% activity after 12 h incubation in the pH range from 4.0 to 8.0 Kitasatospora sp. MK-1785

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.116 617
-
 maltotetraose pH 6.0, 37°C Kitasatospora sp. MK-1785
3.2.1.116 1580
-
 amylose pH 6.0, 37°C Kitasatospora sp. MK-1785
3.2.1.116 1640
-
 maltopentaose pH 6.0, 37°C Kitasatospora sp. MK-1785