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Literature summary extracted from

  • Matsuzawa, T.; Yaoi, K.
    Screening, identification, and characterization of a novel saccharide-stimulated beta-glycosidase from a soil metagenomic library (2017), Appl. Microbiol. Biotechnol., 101, 633-646 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 cellobiose
-
 uncultured microorganism
3.2.1.21 D-galactose
-
 uncultured microorganism
3.2.1.21 D-glucose
-
 uncultured microorganism
3.2.1.21 D-xylose
-
 uncultured microorganism
3.2.1.21 maltose
-
 uncultured microorganism

Application

EC Number Application Comment Organism
3.2.1.21 biofuel production the saccharification yield of rice straw using Trichoderma reesei cellulase is improved by the addition of MeBglD2. These results show that MeBglD2 can be used to improve plant biomass saccharification, because both substrates and products can activate its enzymatic activity uncultured microorganism
3.2.1.23 biofuel production the saccharification yield of rice straw using Trichoderma reesei cellulase is improved by the addition of MeBglD2. These results show that MeBglD2 can be used to improve plant biomass saccharification, because both substrates and products can activate its enzymatic activity uncultured microorganism
3.2.1.38 biofuel production the saccharification yield of rice straw using Trichoderma reesei cellulase is improved by the addition of MeBglD2. These results show that MeBglD2 can be used to improve plant biomass saccharification, because both substrates and products can activate its enzymatic activity uncultured microorganism

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 cloned into the pET-28b vector. His-tagged MeBglD2 is expressed in Escherichia coli BL21(DE3) cells uncultured microorganism
3.2.1.23 cloned into the pET-28b vector. His-tagged MeBglD2 is expressed in Escherichia coli BL21(DE3) cells uncultured microorganism
3.2.1.38 cloned into the pET-28b vector. His-tagged MeBglD2 is expressed in Escherichia coli BL21(DE3) cells uncultured microorganism

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 CuSO4 5 mM, 49% inhibition uncultured microorganism
3.2.1.21 DMSO 10%, 21% inhibition. 25%, 85% inhibition uncultured microorganism
3.2.1.21 EDTA 10 mM, 10% inhibition uncultured microorganism
3.2.1.21 ethanol 10%, 79% inhibition. 25%, 97% inhibition uncultured microorganism

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.47
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 0.49
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 0.65
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 0.77
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 0.83
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 0.98
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 1.3
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.21 2.22
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.21 2.43
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.21 3.93
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.21 6.18
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 7.44
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 7.65
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 8.21
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.21 13.3
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 0.47
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 0.49
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 0.65
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 0.77
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 0.83
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 0.98
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 1.3
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 2.22
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 2.43
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 3.93
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 6.18
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 7.44
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 7.65
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 8.21
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 13.3
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 0.47
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 0.49
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 0.65
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 0.77
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 0.83
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 0.98
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 1.3
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 2.22
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 2.43
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 3.93
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 6.18
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 7.44
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 7.65
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 8.21
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 13.3
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 CaCl2 5 mM, 1.1fold activation uncultured microorganism
3.2.1.21 ZnSO4 5 mM, 1.26fold activation uncultured microorganism

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 uncultured microorganism A0A1E1FFN6
-
-
3.2.1.23 uncultured microorganism A0A1E1FFN6
-
-
3.2.1.38 uncultured microorganism A0A1E1FFN6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21
-
 uncultured microorganism
3.2.1.23
-
 uncultured microorganism
3.2.1.38
-
 uncultured microorganism

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 2.68
-
 substrate: 4-nitrophenyl alpha-D-glucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.21 4.15
-
 substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.21 25.7
-
 substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.21 27.2
-
 substrate: 4-nitrophenyl beta-D-fucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.21 36.2
-
 substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.23 2.68
-
 substrate: 4-nitrophenyl alpha-D-glucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.23 4.15
-
 substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.23 25.7
-
 substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.23 27.2
-
 substrate: 4-nitrophenyl beta-D-fucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.23 36.2
-
 substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.38 2.68
-
 substrate: 4-nitrophenyl alpha-D-glucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.38 4.15
-
 substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.38 25.7
-
 substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.38 27.2
-
 substrate: 4-nitrophenyl beta-D-fucopyranoside, pH 6.0, 60°C uncultured microorganism
3.2.1.38 36.2
-
 substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 6.0, 60°C uncultured microorganism

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl alpha-D-glucopyranoside + H2O 7.4% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucopyranoside + H2O 75% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-fucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 uncultured microorganism 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O 71% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O 11% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.21 cellobiose + H2O
-
 uncultured microorganism 2 beta-D-glucose
-
 ?
3.2.1.21 laminaribiose + H2O
-
 uncultured microorganism 2 beta-D-glucose
-
 ?
3.2.1.21 sophorose + H2O
-
 uncultured microorganism alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.23 4-nitrophenyl alpha-D-glucopyranoside + H2O 7.4% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-fucopyranoside + H2O 75% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-fucopyranose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 uncultured microorganism 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-glucopyranoside + H2O 71% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-glucopyranose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-xylopyranoside + H2O 11% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.23 cellobiose + H2O
-
 uncultured microorganism 2 beta-D-glucose
-
 ?
3.2.1.23 laminaribiose + H2O
-
 uncultured microorganism 2 beta-D-glucose
-
 ?
3.2.1.23 sophorose + H2O
-
 uncultured microorganism alpha-D-glucose + beta-D-glucose
-
 ?
3.2.1.38 4-nitrophenyl alpha-D-glucopyranoside + H2O 7.4% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.38 4-nitrophenyl beta-D-fucopyranoside + H2O 75% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-fucopyranose
-
 ?
3.2.1.38 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 uncultured microorganism 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.38 4-nitrophenyl beta-D-glucopyranoside + H2O 71% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-glucopyranose
-
 ?
3.2.1.38 4-nitrophenyl beta-D-xylopyranoside + H2O 11% of the activity with 4-nitrophenyl beta-D-galactopyranoside uncultured microorganism 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.38 cellobiose + H2O
-
 uncultured microorganism 2 beta-D-glucose
-
 ?
3.2.1.38 laminaribiose + H2O
-
 uncultured microorganism 2 beta-D-glucose
-
 ?
3.2.1.38 sophorose + H2O
-
 uncultured microorganism alpha-D-glucose + beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 MeBglD2 beta-glucosidase/galactosidase/fucosidase activity, cf. 3.2.1.23 and 3.2.1.38 uncultured microorganism
3.2.1.23 MeBglD2 beta-glucosidase/galactosidase/fucosidase activity, cf. 3.2.1.21 and 3.2.1.38 uncultured microorganism
3.2.1.38 MeBglD2 beta-glucosidase/galactosidase/fucosidase activity, cf. 3.2.1.21 and 3.2.1.23 uncultured microorganism

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 31.2
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 34.8
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 91.1
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 95.6
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 108
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 117
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 128
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 129
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 142
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 187
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.21 197
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.21 236
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.21 286
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.21 486
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.21 600
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 31.2
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 34.8
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 91.1
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 95.6
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 108
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 117
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 128
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 129
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 142
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 187
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 197
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 236
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 286
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 486
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 600
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 31.2
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 34.8
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 91.1
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 95.6
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 108
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 117
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 128
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 129
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 142
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 187
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 197
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 236
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 286
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 486
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 600
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 12.5
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 14
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.21 17.3
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 20.7
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 24
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.21 63.7
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 74.5
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.21 96.8
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.21 118
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 124
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.21 129
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 129
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.21 144
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.21 181
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.21 463
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 12.5
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 14
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 17.3
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 20.7
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 24
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 63.7
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 74.5
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.23 96.8
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 118
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 124
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.23 129
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 129
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.23 144
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.23 181
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.23 463
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 12.5
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 14
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 17.3
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 20.7
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 24
-
 4-nitrophenyl beta-D-galactopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 63.7
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 74.5
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, without additive uncultured microorganism
3.2.1.38 96.8
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 118
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 124
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-glucose uncultured microorganism
3.2.1.38 129
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 129
-
 4-nitrophenyl beta-D-glucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism
3.2.1.38 144
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 25 mM cellobiose uncultured microorganism
3.2.1.38 181
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-galactose uncultured microorganism
3.2.1.38 463
-
 4-nitrophenyl beta-D-fucopyranoside pH 6.0, 60°C, additive: 250 mM D-xylose uncultured microorganism