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Literature summary extracted from
- Characterisation of three fungal glucuronoyl esterases on glucuronic acid ester model compounds (2017), Appl. Microbiol. Biotechnol., 101, 5301-5311 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.117 | biofuel production | the enzyme is a promising candidate as auxiliary enzymes to improve saccharification of plant biomass | Sodiomyces alcalophilus |
| 3.1.1.117 | biofuel production | the enzyme is a promising candidate as auxiliary enzymes to improve saccharification of plant biomass | Phanerodontia chrysosporium |
| 3.1.1.117 | biofuel production | the enzyme is a promising candidate as auxiliary enzymes to improve saccharification of plant biomass | Wolfiporia cocos |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.117 | heterologous expression in Pichia pastoris SMD1168H | Sodiomyces alcalophilus |
| 3.1.1.117 | heterologous expression in Pichia pastoris SMD1168H | Phanerodontia chrysosporium |
| 3.1.1.117 | heterologous expression in Pichia pastoris SMD1168H | Wolfiporia cocos |
| 3.1.1.117 | phylogenetic analysis, recombinant expression of His-tagged enzyme in protease-deficient Pichia pastoris strain SMD1168H, subcloning in Escherichia coli strain DH5alpha | Schizophyllum commune |
| 3.1.1.117 | phylogenetic analysis, recombinant expression of His-tagged enzyme in protease-deficient Pichia pastoris strain SMD1168H, subcloning in Escherichia coli strain DH5alpha | Phanerodontia chrysosporium |
| 3.1.1.117 | phylogenetic analysis, recombinant expression of His-tagged enzyme in protease-deficient Pichia pastoris strain SMD1168H, subcloning in Escherichia coli strain DH5alpha | Wolfiporia cocos |
| 3.1.1.117 | phylogenetic analysis, recombinant expression of His-tagged isozymes in protease-deficient Pichia pastoris strain SMD1168H, subcloning in Escherichia coli strain DH5alpha | Lentithecium fluviatile |
| 3.1.1.117 | phylogenetic analysis, recombinant expression of His6-tagged enzyme in protease-deficient Pichia pastoris strain SMD1168H, subcloning in Escherichia coli strain DH5alpha | Sodiomyces alcalophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.117 | additional information | - |
additional information | Michaelis-Menten kinetics | Sodiomyces alcalophilus | |
| 3.1.1.117 | additional information | - |
additional information | Michaelis-Menten kinetics | Phanerodontia chrysosporium | |
| 3.1.1.117 | additional information | - |
additional information | Michaelis-Menten kinetics | Wolfiporia cocos | |
| 3.1.1.117 | 1.7 | - |
benzyl glucuronic acid | pH 6.0, 35°C | Sodiomyces alcalophilus |  |
| 3.1.1.117 | 1.7 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Sodiomyces alcalophilus | |
| 3.1.1.117 | 2.9 | - |
benzyl glucuronic acid | pH 6.0, 35°C | Wolfiporia cocos | |
| 3.1.1.117 | 2.9 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Phanerodontia chrysosporium | |
| 3.1.1.117 | 3.4 | - |
benzyl glucuronic acid | pH 6.0, 35°C | Phanerodontia chrysosporium | |
| 3.1.1.117 | 3.4 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Wolfiporia cocos | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.117 | additional information | the enzyme contains a signal peptide sequence, the cleavage site is between amino acids 17 and 18 for LfGE1, and between amino acids 16 and 17 for LfGE2 and LfGE3 | Lentithecium fluviatile | - |
- |
| 3.1.1.117 | additional information | the enzyme contains a signal peptide sequence, the cleavage site is between amino acids 18 and 19 | Schizophyllum commune | - |
- |
| 3.1.1.117 | additional information | the enzyme contains a signal peptide sequence, the cleavage site is between amino acids 19 and 20 | Sodiomyces alcalophilus | - |
- |
| 3.1.1.117 | additional information | the enzyme contains a signal peptide sequence, the cleavage site is between amino acids 19 and 20 | Phanerodontia chrysosporium | - |
- |
| 3.1.1.117 | additional information | the enzyme contains a signal peptide sequence, the cleavage site is between amino acids 19 and 20 | Wolfiporia cocos | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.117 | additional information | Schizophyllum commune | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Sodiomyces alcalophilus | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Phanerodontia chrysosporium | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Wolfiporia cocos | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Lentithecium fluviatile | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Phanerodontia chrysosporium ATCC MYA-4764 | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Phanerodontia chrysosporium FGSC 9002 | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Wolfiporia cocos MD-104 | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Schizophyllum commune H4-8 | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
| 3.1.1.117 | additional information | Phanerodontia chrysosporium RP-78 | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | ? | - |
- |
|
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.117 | Lentithecium fluviatile | - |
- |
- |
| 3.1.1.117 | Phanerodontia chrysosporium | P0CT87 | - |
- |
| 3.1.1.117 | Phanerodontia chrysosporium | P0CT87 | Sporotrichum pruinosum | - |
| 3.1.1.117 | Phanerodontia chrysosporium ATCC MYA-4764 | P0CT87 | Sporotrichum pruinosum | - |
| 3.1.1.117 | Phanerodontia chrysosporium FGSC 9002 | P0CT87 | Sporotrichum pruinosum | - |
| 3.1.1.117 | Phanerodontia chrysosporium RP-78 | P0CT87 | - |
- |
| 3.1.1.117 | Phanerodontia chrysosporium RP-78 | P0CT87 | Sporotrichum pruinosum | - |
| 3.1.1.117 | Schizophyllum commune | D8QLP9 | - |
- |
| 3.1.1.117 | Schizophyllum commune H4-8 | D8QLP9 | - |
- |
| 3.1.1.117 | Sodiomyces alcalophilus | A0A1D8EJG8 | - |
- |
| 3.1.1.117 | Sodiomyces alcalophilus | A0A1D8EJG8 | Acremonium alcalophilum | - |
| 3.1.1.117 | Wolfiporia cocos | P0CU53 | - |
- |
| 3.1.1.117 | Wolfiporia cocos MD-104 | P0CU53 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.1.117 | glycoprotein | AaGE1 does not contain the Asn-Xaa-Ser/Thr sequon that usually signifies an N-glycosylation site in eukaryotes, but the observed molecular weight of AaGE1 is about 10 kDa higher than calculated, which is due to a heavily O-glycosylated linker region between the CBM1 and the CE15 catalytic domain | Sodiomyces alcalophilus |
| 3.1.1.117 | glycoprotein | enzyme WcGE1 is predicted to contain between two and five sites of the Asn-Xaa-Ser/Thr sequon that usually signifies an N-glycosylation site in eukaryotes. WcGE1 has up to 5 N-glycans | Wolfiporia cocos |
| 3.1.1.117 | glycoprotein | PcGE1 does not contain the Asn-Xaa-Ser/Thr sequon that usually signifies an N-glycosylation site in eukaryotes, but the observed molecular weight of PcGE1 is about 10 kDa higher than calculated, which could be due to a heavily O-glycosylated linker region between the CBM1 and the CE15 catalytic domain | Phanerodontia chrysosporium |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.117 | recombinant enzyme | Sodiomyces alcalophilus |
| 3.1.1.117 | recombinant enzyme | Phanerodontia chrysosporium |
| 3.1.1.117 | recombinant enzyme | Wolfiporia cocos |
| 3.1.1.117 | recombinant His6-tagged enzyme from Pichia pastoris strain SMD1168H by nickel affinity and anion exchange chromatography | Phanerodontia chrysosporium |
| 3.1.1.117 | recombinant His6-tagged enzyme from Pichia pastoris strain SMD1168H by nickel affinity chromatography | Schizophyllum commune |
| 3.1.1.117 | recombinant His6-tagged enzyme from Pichia pastoris strain SMD1168H by nickel affinity chromatography | Sodiomyces alcalophilus |
| 3.1.1.117 | recombinant His6-tagged enzyme from Pichia pastoris strain SMD1168H by nickel affinity chromatography | Wolfiporia cocos |
| 3.1.1.117 | recombinant His6-tagged isozymes from Pichia pastoris strain SMD1168H by nickel affinity chromatography | Lentithecium fluviatile |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.117 | allyl glucuronic acid + H2O | activity is about 20% compared to the activity with benzyl glucuronic acid | Sodiomyces alcalophilus | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl glucuronic acid + H2O | activity is about 30% compared to the activity with benzyl glucuronic acid | Phanerodontia chrysosporium | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl glucuronic acid + H2O | activity is about 80% compared to the activity with benzyl glucuronic acid | Wolfiporia cocos | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl glucuronic acid + H2O | activity is about 30% compared to the activity with benzyl glucuronic acid | Phanerodontia chrysosporium ATCC MYA-4764 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl glucuronic acid + H2O | activity is about 30% compared to the activity with benzyl glucuronic acid | Phanerodontia chrysosporium FGSC 9002 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl glucuronic acid + H2O | activity is about 80% compared to the activity with benzyl glucuronic acid | Wolfiporia cocos MD-104 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl glucuronic acid + H2O | activity is about 30% compared to the activity with benzyl glucuronic acid | Phanerodontia chrysosporium RP-78 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | - |
Phanerodontia chrysosporium | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | low activity | Sodiomyces alcalophilus | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | high activity | Wolfiporia cocos | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | moderate activity | Schizophyllum commune | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | moderate activity | Lentithecium fluviatile | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | - |
Phanerodontia chrysosporium ATCC MYA-4764 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | - |
Phanerodontia chrysosporium FGSC 9002 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | high activity | Wolfiporia cocos MD-104 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | moderate activity | Schizophyllum commune H4-8 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | allyl-D-glucuronate + H2O | - |
Phanerodontia chrysosporium RP-78 | prop-2-en-1-ol + glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl glucuronic acid + H2O | - |
Sodiomyces alcalophilus | benzyl alcohol + glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl glucuronic acid + H2O | - |
Phanerodontia chrysosporium | benzyl alcohol + glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl glucuronic acid + H2O | - |
Wolfiporia cocos | benzyl alcohol + glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl glucuronic acid + H2O | - |
Phanerodontia chrysosporium ATCC MYA-4764 | benzyl alcohol + glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl glucuronic acid + H2O | - |
Phanerodontia chrysosporium FGSC 9002 | benzyl alcohol + glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl glucuronic acid + H2O | - |
Wolfiporia cocos MD-104 | benzyl alcohol + glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl glucuronic acid + H2O | - |
Phanerodontia chrysosporium RP-78 | benzyl alcohol + glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl-D-glucuronate + H2O | - |
Phanerodontia chrysosporium | benzyl alcohol + D-glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl-D-glucuronate + H2O | best substrate | Schizophyllum commune | benzyl alcohol + D-glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl-D-glucuronate + H2O | best substrate | Sodiomyces alcalophilus | benzyl alcohol + D-glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl-D-glucuronate + H2O | best substrate | Wolfiporia cocos | benzyl alcohol + D-glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl-D-glucuronate + H2O | best substrate | Lentithecium fluviatile | benzyl alcohol + D-glucuronic acid | - |
? | |
| 3.1.1.117 | benzyl-D-glucuronate + H2O | best substrate | Schizophyllum commune H4-8 | benzyl alcohol + D-glucuronic acid | - |
? | |
| 3.1.1.117 | methyl glucuronic acid + H2O | activity is about 10% compared to the activity with benzyl glucuronic acid | Phanerodontia chrysosporium | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl glucuronic acid + H2O | activity is about 15% compared to the activity with benzyl glucuronic acid | Wolfiporia cocos | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl glucuronic acid + H2O | activity isabout 5% compared to the activity with benzyl glucuronic acid | Sodiomyces alcalophilus | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl glucuronic acid + H2O | activity is about 10% compared to the activity with benzyl glucuronic acid | Phanerodontia chrysosporium ATCC MYA-4764 | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl glucuronic acid + H2O | activity is about 10% compared to the activity with benzyl glucuronic acid | Phanerodontia chrysosporium FGSC 9002 | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl glucuronic acid + H2O | activity is about 15% compared to the activity with benzyl glucuronic acid | Wolfiporia cocos MD-104 | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl glucuronic acid + H2O | activity is about 10% compared to the activity with benzyl glucuronic acid | Phanerodontia chrysosporium RP-78 | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl-D-glucuronate + H2O | - |
Phanerodontia chrysosporium | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl-D-glucuronate + H2O | very low activity | Sodiomyces alcalophilus | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl-D-glucuronate + H2O | low activity | Schizophyllum commune | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl-D-glucuronate + H2O | low activity | Wolfiporia cocos | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl-D-glucuronate + H2O | low activity | Lentithecium fluviatile | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | methyl-D-glucuronate + H2O | low activity | Schizophyllum commune H4-8 | methanol + glucuronic acid | - |
? | |
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Schizophyllum commune | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Sodiomyces alcalophilus | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Phanerodontia chrysosporium | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Wolfiporia cocos | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Lentithecium fluviatile | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Phanerodontia chrysosporium ATCC MYA-4764 | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Phanerodontia chrysosporium FGSC 9002 | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Wolfiporia cocos MD-104 | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Schizophyllum commune H4-8 | ? | - |
- |
|
| 3.1.1.117 | additional information | in nature, the enzyme hydrolyses ester bonds between aliphatic alcohols in lignin and the 4-O-methyl-D-glucuronic acid side chains of xylan | Phanerodontia chrysosporium RP-78 | ? | - |
- |
|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.117 | ? | x * 44200, recombinant His6-tagged enzyme, SDS-PAGE | Wolfiporia cocos |
| 3.1.1.117 | ? | x * 42800, recombinant His6-tagged enzyme, SDS-PAGE | Schizophyllum commune |
| 3.1.1.117 | ? | x * 44700, recombinant His6-tagged isozyme LfGE1, SDS-PAGE, x * 42100, recombinant His6-tagged isozyme LfGE2, SDS-PAGE, x * 43900, recombinant His6-tagged isozyme LfGE3, SDS-PAGE | Lentithecium fluviatile |
| 3.1.1.117 | ? | x * 50500, recombinant His6-tagged enzyme, SDS-PAGE | Phanerodontia chrysosporium |
| 3.1.1.117 | ? | x * 53100, recombinant His6-tagged enzyme, SDS-PAGE | Sodiomyces alcalophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.117 | 4-O-methyl-glucuronoyl methylesterase | UniProt | Schizophyllum commune |
| 3.1.1.117 | 4-O-methyl-glucuronoyl methylesterase | UniProt | Lentithecium fluviatile |
| 3.1.1.117 | 4-O-methyl-glucuronoyl methylesterase 1 | UniProt | Sodiomyces alcalophilus |
| 3.1.1.117 | 4-O-methyl-glucuronoyl methylesterase 1 | UniProt | Phanerodontia chrysosporium |
| 3.1.1.117 | 4-O-methyl-glucuronoyl methylesterase 1 | UniProt | Wolfiporia cocos |
| 3.1.1.117 | AaGE1 | - |
Sodiomyces alcalophilus |
| 3.1.1.117 | fungal GE | - |
Sodiomyces alcalophilus |
| 3.1.1.117 | fungal GE | - |
Wolfiporia cocos |
| 3.1.1.117 | fungal glucuronoyl esterase | - |
Schizophyllum commune |
| 3.1.1.117 | fungal glucuronoyl esterase | - |
Sodiomyces alcalophilus |
| 3.1.1.117 | fungal glucuronoyl esterase | - |
Phanerodontia chrysosporium |
| 3.1.1.117 | fungal glucuronoyl esterase | - |
Wolfiporia cocos |
| 3.1.1.117 | fungal glucuronoyl esterase | - |
Lentithecium fluviatile |
| 3.1.1.117 | GCE1 | UniProt | Sodiomyces alcalophilus |
| 3.1.1.117 | GCE1 | UniProt | Wolfiporia cocos |
| 3.1.1.117 | glucuronoyl esterase | - |
Schizophyllum commune |
| 3.1.1.117 | glucuronoyl esterase | - |
Sodiomyces alcalophilus |
| 3.1.1.117 | glucuronoyl esterase | - |
Phanerodontia chrysosporium |
| 3.1.1.117 | glucuronoyl esterase | - |
Wolfiporia cocos |
| 3.1.1.117 | glucuronoyl esterase | - |
Lentithecium fluviatile |
| 3.1.1.117 | glucuronoyl esterase 1 | UniProt | Sodiomyces alcalophilus |
| 3.1.1.117 | glucuronoyl esterase 1 | UniProt | Phanerodontia chrysosporium |
| 3.1.1.117 | glucuronoyl esterase 1 | UniProt | Wolfiporia cocos |
| 3.1.1.117 | LfGE1 | - |
Lentithecium fluviatile |
| 3.1.1.117 | LfGE2 | - |
Lentithecium fluviatile |
| 3.1.1.117 | LfGE3 | - |
Lentithecium fluviatile |
| 3.1.1.117 | PcGE1 | - |
Phanerodontia chrysosporium |
| 3.1.1.117 | ScGE2 | - |
Schizophyllum commune |
| 3.1.1.117 | WcGE1 | - |
Wolfiporia cocos |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.117 | 35 | - |
assay at | Schizophyllum commune |
| 3.1.1.117 | 35 | - |
assay at | Sodiomyces alcalophilus |
| 3.1.1.117 | 35 | - |
assay at | Phanerodontia chrysosporium |
| 3.1.1.117 | 35 | - |
assay at | Wolfiporia cocos |
| 3.1.1.117 | 35 | - |
assay at | Lentithecium fluviatile |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.117 | 30 | - |
6 h, no loss of activity | Phanerodontia chrysosporium |
| 3.1.1.117 | 30 | 40 | purified recombinant His6-tagged enzyme, pH 6.0, 6 h, loss of 20% activity | Wolfiporia cocos |
| 3.1.1.117 | 40 | - |
6 h, about 20% loss of activity | Wolfiporia cocos |
| 3.1.1.117 | 40 | - |
purified recombinant His6-tagged enzyme, pH 6.0, stable up to for 6 h | Phanerodontia chrysosporium |
| 3.1.1.117 | 50 | - |
6 h, about 20% loss of activity | Sodiomyces alcalophilus |
| 3.1.1.117 | 50 | - |
6 h, about 20% loss of activity | Phanerodontia chrysosporium |
| 3.1.1.117 | 50 | - |
6 h, about 75% loss of activity | Wolfiporia cocos |
| 3.1.1.117 | 50 | - |
purified recombinant His6-tagged enzyme, pH 6.0, 6 h, loss of 20% activity | Sodiomyces alcalophilus |
| 3.1.1.117 | 50 | - |
purified recombinant His6-tagged enzyme, pH 6.0, 6 h, loss of 20% activity | Phanerodontia chrysosporium |
| 3.1.1.117 | 50 | - |
purified recombinant His6-tagged enzyme, pH 6.0, 6 h, loss of 70% activity | Wolfiporia cocos |
| 3.1.1.117 | 60 | - |
6 h, complete loss of activity | Sodiomyces alcalophilus |
| 3.1.1.117 | 60 | - |
6 h, complete loss of activity | Phanerodontia chrysosporium |
| 3.1.1.117 | 60 | - |
purified recombinant His6-tagged enzyme, pH 6.0, 6 h, inactivation | Sodiomyces alcalophilus |
| 3.1.1.117 | 60 | - |
purified recombinant His6-tagged enzyme, pH 6.0, 6 h, inactivation | Phanerodontia chrysosporium |
| 3.1.1.117 | 70 | - |
6 h, complete loss of activity | Wolfiporia cocos |
| 3.1.1.117 | 70 | - |
purified recombinant His6-tagged enzyme, pH 6.0, 6 h, inactivation | Wolfiporia cocos |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.117 | 0.37 | - |
benzyl glucuronic acid | pH 6.0, 35°C | Wolfiporia cocos | |
| 3.1.1.117 | 0.37 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Phanerodontia chrysosporium | |
| 3.1.1.117 | 0.79 | - |
benzyl glucuronic acid | pH 6.0, 35°C | Sodiomyces alcalophilus | |
| 3.1.1.117 | 0.79 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Sodiomyces alcalophilus | |
| 3.1.1.117 | 1.45 | - |
benzyl glucuronic acid | pH 6.0, 35°C | Phanerodontia chrysosporium | |
| 3.1.1.117 | 1.45 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Wolfiporia cocos | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.117 | 6 | - |
assay at | Schizophyllum commune |
| 3.1.1.117 | 6 | - |
assay at | Sodiomyces alcalophilus |
| 3.1.1.117 | 6 | - |
assay at | Phanerodontia chrysosporium |
| 3.1.1.117 | 6 | - |
assay at | Wolfiporia cocos |
| 3.1.1.117 | 6 | - |
assay at | Lentithecium fluviatile |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.117 | 3 | - |
1 week, about 15% loss of activity, glycine-NaOH buffer | Wolfiporia cocos |
| 3.1.1.117 | 3 | - |
1 week, no loss of activity, glycine-NaOH buffer | Phanerodontia chrysosporium |
| 3.1.1.117 | 4 | - |
1 week, about 20% loss of activity, glycine-NaOH buffer | Phanerodontia chrysosporium |
| 3.1.1.117 | 4 | - |
1 week, about 35% loss of activity, glycine-NaOH buffer | Wolfiporia cocos |
| 3.1.1.117 | 5 | 11 | purified recombinant His6-tagged enzyme, 4°C, 1 week, over 80% activity remaining | Sodiomyces alcalophilus |
| 3.1.1.117 | 5 | - |
1 week, about 25% loss of activity, glycine-NaOH buffer | Phanerodontia chrysosporium |
| 3.1.1.117 | 5 | - |
1 week, about 40% loss of activity, glycine-NaOH buffer | Wolfiporia cocos |
| 3.1.1.117 | 6 | - |
1 week, about 35% loss of activity, glycine-NaOH buffer | Phanerodontia chrysosporium |
| 3.1.1.117 | 6 | - |
1 week, about 40% loss of activity, glycine-NaOH buffer | Wolfiporia cocos |
| 3.1.1.117 | 6 | 11 | 1 week, stable | Sodiomyces alcalophilus |
| 3.1.1.117 | 7 | - |
1 week, no loss of activity, Tris-HCl buffer | Phanerodontia chrysosporium |
| 3.1.1.117 | 7 | - |
1 week, no loss of activity, Tris-HCl buffer | Wolfiporia cocos |
| 3.1.1.117 | 8 | - |
1 week, about 15% loss of activity, Tris-HCl buffer | Wolfiporia cocos |
| 3.1.1.117 | 8 | - |
1 week, no loss of activity, Tris-HCl buffer | Phanerodontia chrysosporium |
| 3.1.1.117 | 9 | - |
1 week, about 15% loss of activity, Tris-HCl buffer | Wolfiporia cocos |
| 3.1.1.117 | 9 | - |
1 week, complete loss of activity, Tris-HCl buffer | Phanerodontia chrysosporium |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.117 | Phanerodontia chrysosporium | sequence calculation | - |
5.7 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.117 | evolution | glucuronoyl esterases (GEs) are members of the carbohydrate esterase 15 family (CE15). Phylogenetic analysis, the evolutionary relationships of CE15 proteins are inferred by maximum likelihood method | Schizophyllum commune |
| 3.1.1.117 | evolution | glucuronoyl esterases (GEs) are members of the carbohydrate esterase 15 family (CE15). Phylogenetic analysis, the evolutionary relationships of CE15 proteins are inferred by maximum likelihood method | Sodiomyces alcalophilus |
| 3.1.1.117 | evolution | glucuronoyl esterases (GEs) are members of the carbohydrate esterase 15 family (CE15). Phylogenetic analysis, the evolutionary relationships of CE15 proteins are inferred by maximum likelihood method | Phanerodontia chrysosporium |
| 3.1.1.117 | evolution | glucuronoyl esterases (GEs) are members of the carbohydrate esterase 15 family (CE15). Phylogenetic analysis, the evolutionary relationships of CE15 proteins are inferred by maximum likelihood method | Wolfiporia cocos |
| 3.1.1.117 | evolution | glucuronoyl esterases (GEs) are members of the carbohydrate esterase 15 family (CE15). Phylogenetic analysis, the evolutionary relationships of CE15 proteins are inferred by maximum likelihood method | Lentithecium fluviatile |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.117 | 0.128 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Phanerodontia chrysosporium | |
| 3.1.1.117 | 0.427 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Wolfiporia cocos | |
| 3.1.1.117 | 0.465 | - |
benzyl-D-glucuronate | pH 6.0, 35°C, recombinant enzyme | Sodiomyces alcalophilus | |
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Release 2023.1 (January 2023)
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