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Literature summary extracted from

  • Davaeifar, S.; Shariati, P.; Tabandeh, F.; Yakhchali, B.
    Isolation and identification of a new Bacillus cereus strain and characterization of its neopullulanase (2015), Appl. Food Biotechnol., 2, 39-45 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.135 biotechnology thermostable debranching enzyme for biotechnology Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.135 ammonium sulfate 0.5 M, 40% inhibition Bacillus cereus
3.2.1.135 Cu2+ 0.2 mM, 86% inhibition Bacillus cereus
3.2.1.135 EDTA 10 mM, 70% inhibition Bacillus cereus
3.2.1.135 Fe2+ 0.2 mM, 21% inhibition Bacillus cereus
3.2.1.135 Mg2+ 0.2 mM, 58% inhibition Bacillus cereus
3.2.1.135 Ni2+ 0.2 mM, 29% inhibition Bacillus cereus
3.2.1.135 SDS 1 mM, complete inhibition Bacillus cereus
3.2.1.135 Urea 3 M, 60% inhibition Bacillus cereus
3.2.1.135 Zn2+ 0.2 mM, 55% inhibition Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.135 extracellular
-
 Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.135 Ca2+ 2 mM, 1.6fold activation Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.135 Bacillus cereus
-
-
-
3.2.1.135 Bacillus cereus SDK2
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.135 13.43
-
 37°C, pH 7 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.135 pullulan + H2O
-
 Bacillus cereus panose + ?
-
 ?
3.2.1.135 pullulan + H2O
-
 Bacillus cereus SDK2 panose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.135 60
-
-
 Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.135 20 70 20°C: about 45% of maximal activity, 70°C: about 65% of maximal activity Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.135 60
-
-
 Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.135 7
-
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.135 6 9 pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity Bacillus cereus