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Literature summary extracted from

  • Maleki, S.; Hrudikova, R.; Zotchev, S.; Ertesvag, H.
    Identification of a new phosphatase enzyme potentially involved in the sugar phosphate stress response in Pseudomonas fluorescens (2017), Appl. Environ. Microbiol., 83, e02361 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.23 expression in Escherichia coli Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.23 Mn2+ addition of MN2+ decreaseds the reaction rate both without and in combination with Mg2x05 Pseudomonas fluorescens
3.1.3.23 NaCl 50 mM, inhibitory Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.23 0.7
-
 D-fructose 6-phosphate pH 7.5, 23°C Pseudomonas fluorescens
3.1.3.23 1.8
-
 D-glucose 6-phosphate pH 7.5, 23°C Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.23 Mg2+ optimum concentration 2.5 mM Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.23 Pseudomonas fluorescens C3K9U8
-
-
3.1.3.23 Pseudomonas fluorescens SBW25 C3K9U8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.23 4-nitrophenyl phosphate + H2O
-
 Pseudomonas fluorescens 4-nitrophenol + phosphate
-
 ?
3.1.3.23 4-nitrophenyl phosphate + H2O
-
 Pseudomonas fluorescens SBW25 4-nitrophenol + phosphate
-
 ?
3.1.3.23 D-fructose 6-phosphate + H2O
-
 Pseudomonas fluorescens D-fructose + phosphate
-
 ?
3.1.3.23 D-fructose 6-phosphate + H2O
-
 Pseudomonas fluorescens SBW25 D-fructose + phosphate
-
 ?
3.1.3.23 D-glucose 6-phosphate + H2O
-
 Pseudomonas fluorescens D-glucose + phosphate
-
 ?
3.1.3.23 D-glucose 6-phosphate + H2O
-
 Pseudomonas fluorescens SBW25 D-glucose + phosphate
-
 ?
3.1.3.23 D-ribose 5-phosphate + H2O preferred substrate Pseudomonas fluorescens D-ribose + phosphate
-
 ?
3.1.3.23 D-ribose 5-phosphate + H2O preferred substrate Pseudomonas fluorescens SBW25 D-ribose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.23 ? x * 29900, calculated from sequence and SDS-PAGE, recombinant protein including His-tag Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.1.3.23 PFLU2693
-
 Pseudomonas fluorescens
3.1.3.23 SPP
-
 Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.23 9.6
-
 D-fructose 6-phosphate pH 7.5, 23°C Pseudomonas fluorescens
3.1.3.23 11.4
-
 D-glucose 6-phosphate pH 7.5, 23°C Pseudomonas fluorescens

General Information

EC Number General Information Comment Organism
3.1.3.23 physiological function deletion of the Spp gene does not affect growth of the wild-type strain on fructose, but the gene cannot be deleted in the alginate-producing strain. Sugar phosphate accumulation in Spp mutant strains causes diminished growth in some of the strains, which is partially relieved by Spp overexpression. Overexpression of Spp in fructose-grown alginate-producing strains negatively affects both growth and alginate production Pseudomonas fluorescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.23 6.4
-
 D-glucose 6-phosphate pH 7.5, 23°C Pseudomonas fluorescens
3.1.3.23 13
-
 D-fructose 6-phosphate pH 7.5, 23°C Pseudomonas fluorescens