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Literature summary extracted from

  • Amin, F.; Bhatti, H.N.; Bilal, M.; Asgher, M.
    Purification, kinetic, and thermodynamic characteristics of an exo-polygalacturonase from Penicillium notatum with industrial perspective (2017), Appl. Biochem. Biotechnol., 183, 426-443 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.67 Triton X-100 about 115% activity at 1% (v/v) Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Al3+ about 65% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 Ca2+ about 60% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 Cd2+ about 65% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 Citric acid about 90% residual activity at 1% (v/v) Penicillium chrysogenum
3.2.1.67 Cr3+ about 63% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 Cu2+ about 70% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 EDTA about 42% residual activity at 1% (v/v) Penicillium chrysogenum
3.2.1.67 Hg2+ about 50% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 Mg2+ about 65% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 Ni2+ about 75% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 Pb2+ about 75% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 SDS about 45% residual activity at 1% (w/v) Penicillium chrysogenum
3.2.1.67 Sodium azide
-
 Penicillium chrysogenum
3.2.1.67 Sr2+ about 90% residual activity at 2 mM Penicillium chrysogenum
3.2.1.67 Tween 80 about 90% residual activity at 1% (v/v) Penicillium chrysogenum
3.2.1.67 Urea
-
 Penicillium chrysogenum
3.2.1.67 Zn2+ about 63% residual activity at 2 mM Penicillium chrysogenum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Penicillium chrysogenum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.67 Co2+ about 110% activity at 2 mM Penicillium chrysogenum
3.2.1.67 Mn2+ about 125% activity at 2 mM Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Penicillium chrysogenum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 ammonium sulfate precipitation, DEAE cellulose column chromatography, and Superdex G-75 gel filtration Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 polygalacturonic acid + H2O
-
 Penicillium chrysogenum galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 ? x * 20000, isoform exo-PGII, SDS-PAGE Penicillium chrysogenum
3.2.1.67 ? x * 85000, isoform exo-PGI, SDS-PAGE Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exo-PGI isoform Penicillium chrysogenum
3.2.1.67 exo-PGII isoform Penicillium chrysogenum
3.2.1.67 exo-polygalacturonanase
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 50
-
 a significant loss in enzymatic activity is observed at temperatures greater than 50°C Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 55 80 phase I of isoform exo-PGI shows half-lives of 6.83 and 2.39 min at 55°C and 80°C, respectively, whereas phase II of the enzyme exhibits a half-life of 63.57 and 22.72 min at 55°C and 80°C, respectively Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 6
-
-
 Penicillium chrysogenum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 4.5 6 the purified enzyme exhibits good stability in the pH range of 4.5-6.0 for 24 h Penicillium chrysogenum