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Literature summary extracted from
- Characterization of a thermotolerant phytase produced by Rhizopus microsporus var. microsporus biofilm on an inert support using sugarcane bagasse as carbon source (2016), Appl. Biochem. Biotechnol., 179, 610-624 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | additional information | poor effect by EDTA at 1-2 mM | Rhizopus microsporus |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | additional information | phytases show great potential for application in different sectors such as in animal nutrition, human nutrition, aquaculture, and pharmacology. These enzymes are considered as a green feed additive that can be used to neutralize the antinutritional effects of phytate, thereby increasing the bioavailability of phosphorus and other minerals. They also contribute to the reduction of environmental pollution by phosphorus | Rhizopus microsporus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | arsenate | - |
Rhizopus microsporus |  |
| 3.1.3.8 | Co2+ | 45% inhibition at 2 mM | Rhizopus microsporus | |
| 3.1.3.8 | Cu2+ | 11.5% inhibition at 2 mM | Rhizopus microsporus | |
| 3.1.3.8 | KCl | 50% inhibition at 2 mM | Rhizopus microsporus | |
| 3.1.3.8 | additional information | poor effect by EDTA at 1-2 mM | Rhizopus microsporus | |
| 3.1.3.8 | potassium phosphate | complete inhibition at 1-5 mM | Rhizopus microsporus | |
| 3.1.3.8 | Sodium phosphate | almost complete inhibition at 0.25-1.0 mM | Rhizopus microsporus | |
| 3.1.3.8 | Zn2+ | 85% inhibition at 2 mM | Rhizopus microsporus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.04 | - |
4-nitrophenyl phosphate | pH 5.0, 45°C | Rhizopus microsporus | |
| 3.1.3.8 | 0.72 | - |
myo-inositol hexakisphosphate | pH 4.5, 45°C | Rhizopus microsporus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.8 | extracellular | - |
Rhizopus microsporus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | activates 43% at 2 mM | Rhizopus microsporus | |
| 3.1.3.8 | Ca2+ | activates about 9% at 2 mM | Rhizopus microsporus | |
| 3.1.3.8 | Fe2+ | activates about 10% at 0.4 mM | Rhizopus microsporus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Rhizopus microsporus | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Rhizopus microsporus | - |
var. microsporus, isolated from Brazilian soil | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | native enzyme 4.18fold by anion and cation exchange chromatography | Rhizopus microsporus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | biofilm | thermotolerant phytase is produced by Rhizopus microsporus var. microsporus biofilm on an inert support using sugarcane bagasse as carbon source. The enzyme production is strictly regulated by the phosphorus content with optimal production at 0.5 mM of sodium phytate and KH2PO4 | Rhizopus microsporus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 2285 | - |
purified enzyme, pH 4.5, 50°C | Rhizopus microsporus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | 4-nitrophenyl phosphate + H2O | - |
Rhizopus microsporus | 4-nitrophenol + phosphate | - |
? | |
| 3.1.3.8 | additional information | the phytase demonstrates high substrate specificity for sodium phytate. The enzyme also hydrolyzes 4-nitrophenyl phosphate, ATP, AMPc, glucose 6-phosphate, glucose 1-phosphate, and UDPG | Rhizopus microsporus | ? | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Rhizopus microsporus | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | i.e. phytate | Rhizopus microsporus | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | ? | x * 35400, extracellular enzyme, SDS-PAGE | Rhizopus microsporus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate phosphohydrolase | - |
Rhizopus microsporus |
| 3.1.3.8 | RMPhy1 | - |
Rhizopus microsporus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 55 | - |
- |
Rhizopus microsporus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 30 | 65 | over 50% of maximal activity within this range, profile overview | Rhizopus microsporus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 30 | 40 | purified enzyme, highly stable at | Rhizopus microsporus |
| 3.1.3.8 | 60 | - |
purified enzyme, half-life is 115 min | Rhizopus microsporus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 4.5 | - |
- |
Rhizopus microsporus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 2 | 9 | over 70% of maximal activity within this range, profile overview | Rhizopus microsporus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.1.3.8 | Rhizopus microsporus | phytase production by Rhizopus microsporus var. microsporus biofilm is stimulated by the phosphorus source in the medium | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | evolution | on the basis of the site at which phytate dephosphorization begins, phytases can be grouped into three classes: 3-phytases (EC 3.1.3.8), 6-phytases (EC 3.1.3.26), and 5-phytases (EC 3.1.3.72). The fungal phytases are included in the 3-phytases class, in which the dephosphorization starts at the third phosphate group. Because of their catalytic mechanism, the fungal phytases belong to the histidine acid phosphatase class | Rhizopus microsporus |
| 3.1.3.8 | physiological function | phytases (myo-inositol hexakisphosphate phosphohydrolases) are enzymes that compose a special class of phosphatases. Phytases catalyze the hydrolysis of the phosphomonoester bonds of phytate (salts of myo-inositol 1,2,3,4,5,6-hexakis dihydrogen phosphate), releasing lower forms of myo-inositol and inorganic phosphates. Regulation of enzymatic synthesis by phosphate is coordinated by the PHO pathwa | Rhizopus microsporus |
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