Literature summary extracted from
Yuan, M.; Yang, X.; Li, Y.; Liu, H.; Pu, J.; Zhan, C.G.; Liao, F.
Facile alkaline lysis of Escherichia coli cells in high-throughput mode for screening enzyme mutants arylsulfatase as an example (2016), Appl. Biochem. Biotechnol., 179, 545-557 .
Application
EC Number |
Application |
Comment |
Organism |
---|
3.1.6.1 |
analysis |
high-throughput screen for mutants expressed in Escherichia coli. The alkaline lysis buffer is 1.0 M Tris-HCl at pH 9.0 plus 0.1 % Tween-20 and 2.0 mM 4-aminobenzamidine, mixed with cell suspension at 8:1 to 12:1 ratio for continuous agitation of mixtures in 96-well plates under room temperature. The enzmye tolerates final 0.1 % Tween-20, and activities in lysates are steady from 3 to 9 h and comparable to sonication treatment but better than freezing-thawing |
Pseudomonas aeruginosa |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.6.1 |
exprtession in Escherichia coli |
Pseudomonas aeruginosa |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.6.1 |
M72Q |
2fold increase in activity compared with starting mutant G138S |
Pseudomonas aeruginosa |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.6.1 |
Pseudomonas aeruginosa |
- |
- |
- |