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Literature summary extracted from

  • Grinkevich, P.; Iermak, I.; Luedtke, N.A.; Mesters, J.R.; Ettrich, R.; Ludwig, J.
    pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I (2016), Acta Crystallogr. Sect. F, 72, 672-676 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.3 pHluorin-assisted expression of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.21.3 sitting-drop vapour-diffusion technique, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I. The shortest of the three encompassed HsdR residues 887-1038 and yielded crystals that belonged to the orthorhombic space group C2221, with unit-cell parameters a = 83.42, b = 176.58, c = 126.03 A, alpha = beta = gamma = 90.0 and two molecules in the asymmetric unit. X-ray diffraction data are collected to a resolution of 2.45 A Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.3 Escherichia coli Q304R3 HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.3 pHluorin-assisted purification of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I Escherichia coli

Subunits

EC Number Subunits Comment Organism
3.1.21.3 More the HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.21.3 type I restriction-modification system EcoR124I
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Escherichia coli