Literature summary extracted from
Grinkevich, P.; Iermak, I.; Luedtke, N.A.; Mesters, J.R.; Ettrich, R.; Ludwig, J.
pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I (2016), Acta Crystallogr. Sect. F, 72, 672-676 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.21.3 |
pHluorin-assisted expression of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I |
Escherichia coli |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.1.21.3 |
sitting-drop vapour-diffusion technique, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I. The shortest of the three encompassed HsdR residues 887-1038 and yielded crystals that belonged to the orthorhombic space group C2221, with unit-cell parameters a = 83.42, b = 176.58, c = 126.03 A, alpha = beta = gamma = 90.0 and two molecules in the asymmetric unit. X-ray diffraction data are collected to a resolution of 2.45 A |
Escherichia coli |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.21.3 |
Escherichia coli |
Q304R3 |
HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.21.3 |
pHluorin-assisted purification of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I |
Escherichia coli |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.21.3 |
More |
the HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits |
Escherichia coli |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.21.3 |
type I restriction-modification system EcoR124I |
- |
Escherichia coli |