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Literature summary extracted from

  • Feng, Y.; Wang, Y.; Liu, J.; Liu, Y.; Cao, X.; Xue, S.
    Structural insight into acyl-ACP thioesterase toward substrate specificity design (2017), ACS Chem. Biol., 12, 2830-2836 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 expressed in Escherichia coli BL21(DE3) cells Umbellularia californica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.2.14 hanging drop vapor diffusion method, using 0.2 M (NH4)2SO4, 0.1 M Tris-HCl pH 8.5, 20% (w/v) PEG3350 Umbellularia californica

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.14 F201W the mutant shows 14.1% specific activity compared to the wild type enzyme Umbellularia californica
3.1.2.14 M163W almost inactive Umbellularia californica
3.1.2.14 M197R the mutant shows 39.8% specific activity compared to the wild type enzyme Umbellularia californica
3.1.2.14 N283A almost inactive Umbellularia californica
3.1.2.14 R199H the mutant shows 0.4% specific activity compared to the wild type enzyme Umbellularia californica
3.1.2.14 S219W almost inactive Umbellularia californica
3.1.2.14 T137G the mutant demonstrates comparative relative activity on C14 substrates compared to C12 substrates in vitro and achieves C14 fatty acid content up to 40% in contrast to 10% C14 from the wild type Umbellularia californica
3.1.2.14 T137Y inactive Umbellularia californica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.14 chloroplast
-
 Umbellularia californica 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.14 lauroyl-[acyl-carrier protein] + H2O Umbellularia californica
-
 [acyl-carrier protein] + laurate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Umbellularia californica Q41635
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.14 Ni-NTA column chromatography Umbellularia californica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 caproyl-[acyl-carrier protein] + H2O very low activity Umbellularia californica [acyl-carrier protein] + caproate
-
 ?
3.1.2.14 lauroyl-[acyl-carrier protein] + H2O
-
 Umbellularia californica [acyl-carrier protein] + laurate
-
 ?
3.1.2.14 lauroyl-[acyl-carrier protein] + H2O high activity Umbellularia californica [acyl-carrier protein] + laurate
-
 ?
3.1.2.14 myristoyl-[acyl-carrier protein] + H2O very low activity Umbellularia californica [acyl-carrier protein] + myristate
-
 ?
3.1.2.14 palmitoyl-[acyl-carrier protein] + H2O very low activity Umbellularia californica [acyl-carrier protein] + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.14 homodimer x-ray crystallography Umbellularia californica

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 acyl-ACP-thioesterase
-
 Umbellularia californica
3.1.2.14 C12-specific thioesterase
-
 Umbellularia californica
3.1.2.14 FatB
-
 Umbellularia californica