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Literature summary extracted from

  • Anand, G.; Yadav, S.; Yadav, D.
    Purification and characterization of polygalacturonase from Aspergillus fumigatus MTCC 2584 and elucidating its application in retting of Crotalaria juncea fiber (2016), 3 Biotech, 6, 201 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Ag+ 34% residual activity at 1 mM Aspergillus fumigatus
3.2.1.15 Ca2+ 29.5% residual activity at 1 mM Aspergillus fumigatus
3.2.1.15 EDTA 85.7% residual activity at 1 mM Aspergillus fumigatus
3.2.1.15 Hg2+ 64.2% residual activity at 1 mM Aspergillus fumigatus
3.2.1.15 additional information KMnO4 has no effect on enzyme activity Aspergillus fumigatus
3.2.1.15 potassium ferrocyanide 64.2% residual activity at 1 mM Aspergillus fumigatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Cu2+ 163% activity at 1 mM Aspergillus fumigatus
3.2.1.15 K+ 135.5% activity at 1 mM Aspergillus fumigatus
3.2.1.15 additional information Mn2+, Na+, and Zn+ have no effect on enzyme activity Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Aspergillus fumigatus
-
-
-
3.2.1.15 Aspergillus fumigatus MTCC 2584
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 acetone precipitation and Sephadex G-100 gel filtration Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 polygalacturonate + H2O
-
 Aspergillus fumigatus galacturonate
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Aspergillus fumigatus MTCC 2584 galacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 43000, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endo-PG
-
 Aspergillus fumigatus
3.2.1.15 endo-polygalacturonase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 30
-
-
 Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 10 60 about 50% activity at 10°C, about 60% activity at 20°C, 100% activity at 30°C, about 45% activity at 40°C, about 40% activity at 50°C, about 35% activity at 60°C, no activity at 70°C Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 10 30 the enzyme remains stable for 2 h at 10-30°C and shows about 50% and 25% activity after 2 h at 40°C and 60°C, respectively Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.15 44
-
 Polygalacturonate at pH 10.0 and 30°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 10
-
-
 Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 8 11 more than 60% activity between pH 8.0 and 11.0, no activity at pH 6.0 and below Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 7 11 the enzyme remains stable for 2 h at pH 7.0-11.0 Aspergillus fumigatus