EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.165 | EDTA | 120% activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | NaCl | the optimum NaCl concentration for the enzyme is 250 mM | Aspergillus fumigatus |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.165 | electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme retains more than 50% of its catalytic activity after 10 batches of uses in aqueous solution | Aspergillus fumigatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.165 | 2-Hydroxy-5-nitrobenzyl bromide | 65% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | 4-chloromercuribenzoate | 88% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Ba2+ | 98% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Ca2+ | 88% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Cd2+ | 90% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Co2+ | 32% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Cu2+ | 98% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | dithiothreitol | 90% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Fe2+ | 87% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Hg2+ | 76% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | iodoacetate | 93% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Mg2+ | 95% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Mn2+ | 98% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | N-ethylmaleimide | 66% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Ni2+ | 87% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Triton X-100 | 88% residual activity at 1 mM | Aspergillus fumigatus | |
3.2.1.165 | Tween 20 | 76% residual activity at 1 mM | Aspergillus fumigatus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.165 | Zn2+ | 102% activity at 1 mM | Aspergillus fumigatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.165 | chitosan + H2O | Aspergillus fumigatus | - |
D-glucosamine + ? | - |
? | |
3.2.1.165 | chitosan + H2O | Aspergillus fumigatus IIT-004 | - |
D-glucosamine + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.165 | Aspergillus fumigatus | - |
- |
- |
3.2.1.165 | Aspergillus fumigatus IIT-004 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.165 | acetone fractionation, DEAE Sepharose column chromatography, CM-Sepharose column chromatography, and Superdex 200 gel filtration | Aspergillus fumigatus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.165 | mycelium | - |
Aspergillus fumigatus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 0.5 | - |
crude enzyme, at pH 5.5 and 40°C | Aspergillus fumigatus |
3.2.1.165 | 13 | - |
after 26fold purification, at pH 5.5 and 40°C | Aspergillus fumigatus |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.165 | 25°C, electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme, 30 days, 40% loss of activity | Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.165 | chitin + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
3.2.1.165 | chitin + H2O | - |
Aspergillus fumigatus IIT-004 | ? | - |
? | |
3.2.1.165 | chitosan + H2O | - |
Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
3.2.1.165 | chitosan + H2O | chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
3.2.1.165 | chitosan + H2O | chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
3.2.1.165 | chitosan + H2O | chitosan with more than 70% degree of acetylation, 43% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
3.2.1.165 | chitosan + H2O | chitosan with more than 80% degree of acetylation, 68% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
3.2.1.165 | chitosan + H2O | - |
Aspergillus fumigatus IIT-004 | D-glucosamine + ? | - |
? | |
3.2.1.165 | chitosan + H2O | chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation | Aspergillus fumigatus IIT-004 | D-glucosamine + ? | - |
? | |
3.2.1.165 | chitosan + H2O | chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation | Aspergillus fumigatus IIT-004 | D-glucosamine + ? | - |
? | |
3.2.1.165 | colloidal chitosan + H2O | 56% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
3.2.1.165 | crab shell chitosan + H2O | more than 90% degree of acetylation, 100% activity | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
3.2.1.165 | D-glucosamine + N-acetyl-D-glucosamine | 15% (w/v) ammonium sulfate is optimum for catalyzing transglycosylation reaction | Aspergillus fumigatus | chitodimer | - |
? | |
3.2.1.165 | glycol chitosan + H2O | 12% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
3.2.1.165 | additional information | no activity with flaked chitin, colloidal chitin and cellulose | Aspergillus fumigatus | ? | - |
? | |
3.2.1.165 | additional information | no activity with flaked chitin, colloidal chitin and cellulose | Aspergillus fumigatus IIT-004 | ? | - |
? | |
3.2.1.165 | shrimp shell chitosan + H2O | more than 90% degree of acetylation, 57% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.165 | ? | x * 64000, SDS-PAGE | Aspergillus fumigatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.165 | exo-beta-D-glucosaminidase | - |
Aspergillus fumigatus |
3.2.1.165 | exochitosanase | - |
Aspergillus fumigatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 40 | - |
free enzyme | Aspergillus fumigatus |
3.2.1.165 | 50 | - |
electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme | Aspergillus fumigatus |
3.2.1.165 | 50 | - |
optimum for transglycosylation reaction | Aspergillus fumigatus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 50 | - |
the enzyme remains stable at up to 50°C for 32 h | Aspergillus fumigatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.165 | 3000 | - |
crab shell chitosan | at pH 5.5 and 40°C | Aspergillus fumigatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.165 | 5.5 | - |
free enzyme | Aspergillus fumigatus |
3.2.1.165 | 5.8 | - |
electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme | Aspergillus fumigatus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.165 | Aspergillus fumigatus | isoelectric focusing | - |
6.5 |