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Literature summary extracted from
- Crystallization and X ray diffraction data analyses of the enzyme urocanate hydratase from Trypanosoma cruzi (2016), Rev. Virt. Quim., 8, 678-686 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.49 | the recombinant C-terminally His6-tagged protein is expressed in Escherichia coli | Trypanosoma cruzi |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.49 | the enzyme is crystallized in several conditions, with the best crystals obtained with 0.04 M dipotassium hydrogen phosphate, 16.00 % (w/v) polyethylene glycol 8000 and 22.0% (v/v) glycerol. X ray diffraction data are collected to 2.61 A resolution. The crystals belong to the monoclinic space group P21, with unit cell parameters a = 85.12, b = 137.63, c = 117.69 A, beta = 94.69° and are suitable for molecular replacement phasing | Trypanosoma cruzi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.49 | Trypanosoma cruzi | Q4D9S6 | - |
- |
| 4.2.1.49 | Trypanosoma cruzi CL Brener | Q4D9S6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.49 | - |
Trypanosoma cruzi |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.1.49 | metabolism | the enzyme is involved in histidine degradation and is essential for the catabolic conversion of urocanate to 4-imidazolone-5-propionate, which finally yields glutamate and further glutamine and tricarboxylic acid cycle intemediates such as 2-oxo-pentanedioate | Trypanosoma cruzi |
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Release 2023.1 (January 2023)
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