EC Number | Cloned (Comment) | Organism |
---|---|---|
4.4.1.21 | expression in Escherichia coli | Lactiplantibacillus plantarum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.21 | Cu2+ | 1 mM, 6.1% of initial activity, respectively; 1 mM, 7.5% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | glycerol | 1 mM, 0.1% of initial activity, respectively; 1 mM, 8% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Ni2+ | 1 mM, 23% of initial activity, respectively; 1 mM, 7.8% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Tween-20 | 1 mM, 85% of initial activity, respectively; 1 mM, 86% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Zn2+ | 1 mM, 19.8% of initial activity, respectively; 1 mM, 19% of initial activity, respectively | Lactiplantibacillus plantarum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.21 | 0.88 | - |
S-ribosylhomocysteine | isoform LuxS2, pH 8.0, 37°C | Lactiplantibacillus plantarum | |
4.4.1.21 | 1.23 | - |
S-ribosylhomocysteine | isoform LuxS1, pH 8.0, 45°C | Lactiplantibacillus plantarum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.21 | Al3+ | 1 mM, 110% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Al3+ | 1 mM, 115% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Ca2+ | 1 mM, 108% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Ca2+ | 1 mM, 19% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | EDTA | 1 mM, 113% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | EDTA | 1 mM, 123% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Fe2+ | 1 mM, 110% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Fe2+ | 1 mM, 99% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Mn2+ | 1 mM, 103% of initial activity, respectively | Lactiplantibacillus plantarum | |
4.4.1.21 | Mn2+ | 1 mM, 114% of initial activity, respectively | Lactiplantibacillus plantarum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.21 | Lactiplantibacillus plantarum | ETF12584 | isoform LuxS1 | - |
4.4.1.21 | Lactiplantibacillus plantarum | ETF12641 | isoform LuxS2 | - |
4.4.1.21 | Lactiplantibacillus plantarum YM-4-3 | ETF12584 | isoform LuxS1 | - |
4.4.1.21 | Lactiplantibacillus plantarum YM-4-3 | ETF12641 | isoform LuxS2 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.21 | additional information | both isoforms LuxS1 and LuxS2 can effectively convert S-ribosylhomocysteine to autoinducer AI-2 and homocysteine, and they may have redundant functions | Lactiplantibacillus plantarum | ? | - |
? | |
4.4.1.21 | additional information | both isoforms LuxS1 and LuxS2 can effectively convert S-ribosylhomocysteine to autoinducer AI-2 and homocysteine, and they may have redundant functions | Lactiplantibacillus plantarum YM-4-3 | ? | - |
? | |
4.4.1.21 | S-ribosylhomocysteine | - |
Lactiplantibacillus plantarum | L-homocysteine + 4,5-dihydroxy-2,3-pentanedione | - |
? | |
4.4.1.21 | S-ribosylhomocysteine | - |
Lactiplantibacillus plantarum YM-4-3 | L-homocysteine + 4,5-dihydroxy-2,3-pentanedione | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.21 | ? | x * 17400, calculated from sequence, x * 22000, SDS-PAGE, recombinant protein including His-tag | Lactiplantibacillus plantarum |
4.4.1.21 | ? | x * 17500, calculated from sequence, x * 22000, SDS-PAGE, recombinant protein including His-tag | Lactiplantibacillus plantarum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.4.1.21 | 37 | - |
isoform LuxS2 | Lactiplantibacillus plantarum |
4.4.1.21 | 45 | - |
isoform LuxS1 | Lactiplantibacillus plantarum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.21 | 0.34 | - |
S-ribosylhomocysteine | isoform LuxS2, pH 8.0, 37°C | Lactiplantibacillus plantarum | |
4.4.1.21 | 0.47 | - |
S-ribosylhomocysteine | isoform LuxS1, pH 8.0, 45°C | Lactiplantibacillus plantarum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.4.1.21 | Lactiplantibacillus plantarum | isoform LuxS1, calculated from sequence | - |
6.3 |
4.4.1.21 | Lactiplantibacillus plantarum | isoform LuxS2, calculated from sequence | - |
6.3 |