BRENDA - Enzyme Database show

A dynamic Asp-Arg interaction is essential for catalysis in microsomal prostaglandin E2 synthase

Brock, J.S.; Hamberg, M.; Balagunaseelan, N.; Goodman, M.; Morgenstern, R.; Strandback, E.; Samuelsson, B.; Rinaldo-Matthis, A.; Haeggstroem, J.Z.; Proc. Natl. Acad. Sci. USA 113, 972-977 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expressed in Pichia pastoris
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.3
D49N
the mutant shows severely reduced activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
R126K
the mutant shows severely reduced activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
R126Q
the mutant shows severely reduced activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
S127A
the mutant shows about wild type activity
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
Prostaglandin H2
Homo sapiens
-
Prostaglandin E2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Homo sapiens
O14684
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
Ni-affinity column chromatography
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
749180
Homo sapiens
Prostaglandin E2
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.3
glutathione
dependent on
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expressed in Pichia pastoris
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.3
glutathione
dependent on
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.3
D49N
the mutant shows severely reduced activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
R126K
the mutant shows severely reduced activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
R126Q
the mutant shows severely reduced activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
S127A
the mutant shows about wild type activity
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
Prostaglandin H2
Homo sapiens
-
Prostaglandin E2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
Ni-affinity column chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
749180
Homo sapiens
Prostaglandin E2
-
-
-
?