BRENDA - Enzyme Database show

Arg126 and Asp49 are essential for the catalytic function of microsomal prostaglandin E2 synthase 1 and Ser127 is not

Raouf, J.; Rafique, N.; Goodman, M.; Idborg, H.; Bergqvist, F.; Armstrong, R.; Jakobsson, P.; Morgenstern, R.; Spahiu, L.; PLoS ONE 11, e163600 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expressed in Escherichia coli BL21Star (DE) pLysS cells and SF9 insect cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.3
D49A
inactive
Homo sapiens
5.3.99.3
R126A
inactive
Homo sapiens
5.3.99.3
R126L
inactive
Homo sapiens
5.3.99.3
R73A
the mutant retains 50% activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
R73L
inactive
Homo sapiens
5.3.99.3
S127A
the mutant retains 63% activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
S127C
the mutant displays 80% lowered activity
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
Prostaglandin H2
Homo sapiens
-
Prostaglandin E2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Homo sapiens
O14684
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
Ni-NTA column chromatography and Sephacryl gel filtration
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
749100
Homo sapiens
Prostaglandin E2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
?
x * 17000, SDS-PAGE
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expressed in Escherichia coli BL21Star (DE) pLysS cells and SF9 insect cells
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.3
D49A
inactive
Homo sapiens
5.3.99.3
R126A
inactive
Homo sapiens
5.3.99.3
R126L
inactive
Homo sapiens
5.3.99.3
R73A
the mutant retains 50% activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
R73L
inactive
Homo sapiens
5.3.99.3
S127A
the mutant retains 63% activity compared to the wild type enzyme
Homo sapiens
5.3.99.3
S127C
the mutant displays 80% lowered activity
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
Prostaglandin H2
Homo sapiens
-
Prostaglandin E2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
Ni-NTA column chromatography and Sephacryl gel filtration
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
749100
Homo sapiens
Prostaglandin E2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
?
x * 17000, SDS-PAGE
Homo sapiens