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Literature summary extracted from

  • Hidalgo, J.; Latorre, P.; Carrodeguas, J.A.; Velazquez-Campoy, A.; Sancho, J.; Lopez-Buesa, P.
    Inhibition of pig phosphoenolpyruvate carboxykinase isoenzymes by 3-mercaptopicolinic acid and novel inhibitors (2016), PLoS ONE, 11, e0159002 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.32 M139L natural variant, leading to different kinetic properties Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 3-Mercaptopicolinic acid
-
 Sus scrofa
4.1.1.32 N'1-([5-[1-methyl-5-(trifluoromethyl)-1H-pyrazol-3-yl]-2-thienyl]methylidene)-2,4-dichlorobenzene-1-carbohydrazide compound exhibits significantly different inhibitory effects on wild-type and natural variant M139L, it enhances threefold the apparent Km value for GTP in wild-type, and reduces Km value for M139L from 99 to 69 microM Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 cytosol
-
 Sus scrofa 5829
-

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Sus scrofa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GDP + phosphoenolpyruvate + CO2
-
 Sus scrofa GTP + oxaloacetate
-
 r
4.1.1.32 GTP + oxaloacetate
-
 Sus scrofa GDP + phosphoenolpyruvate + CO2
-
 r

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.32 0.016
-
 3-Mercaptopicolinic acid wild-type, substrate phosphoenolpyruvate, pH 7.4, temperature not specified in the publication Sus scrofa
4.1.1.32 0.018
-
 3-Mercaptopicolinic acid wild-type, substrate oxaloacetate, pH 7.4, temperature not specified in the publication Sus scrofa
4.1.1.32 0.019
-
 3-Mercaptopicolinic acid mutant M139L, substrate phosphoenolpyruvate, pH 7.4, temperature not specified in the publication Sus scrofa
4.1.1.32 0.029
-
 3-Mercaptopicolinic acid mutant M139L, substrate oxaloacetate, pH 7.4, temperature not specified in the publication Sus scrofa
4.1.1.32 0.045
-
 3-Mercaptopicolinic acid mutant M139L, substrate GDP, pH 7.4, temperature not specified in the publication Sus scrofa
4.1.1.32 0.045
-
 3-Mercaptopicolinic acid wild-type, substrate GDP, pH 7.4, temperature not specified in the publication Sus scrofa
4.1.1.32 0.273
-
 3-Mercaptopicolinic acid wild-type, substrate GTP, pH 7.4, temperature not specified in the publication Sus scrofa
4.1.1.32 0.873
-
 3-Mercaptopicolinic acid mutant M139L, substrate GTP, pH 7.4, temperature not specified in the publication Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.1.32 0.065
-
 wild-type, pH 7.4, temperature not specified in the publication Sus scrofa 3-Mercaptopicolinic acid