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Literature summary extracted from

  • Sigdel, S.; Singh, R.; Kim, T.S.; Li, J.; Kim, S.Y.; Kim, I.W.; Jung, W.S.; Pan, C.H.; Kang, Y.C.; Lee, J.K.
    Characterization of a mannose-6-phosphate isomerase from Bacillus amyloliquefaciens and its application in fructose-6-phosphate production (2015), PLoS ONE, 10, e0131585 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.8 expression in Escherichia coli Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 EDTA 10 mM, complete loss of activity Bacillus amyloliquefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.8 7.62
-
 D-mannose 6-phosphate pH 7.5, 70°C Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.8 Co2+ most effective activator of EDTA-inactivated enzyme, 1.7fold activation at 0.5 mM compared to initial activity Bacillus amyloliquefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.8 35000
-
 gel filtration Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.8 Bacillus amyloliquefaciens
-
-
-
5.3.1.8 Bacillus amyloliquefaciens DSM 7
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.8 81
-
 substrate D-mannose 6-phosphate, pH 7.5, 70°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.8 D-Mannose 6-phosphate
-
 Bacillus amyloliquefaciens D-Fructose 6-phosphate
-
 ?
5.3.1.8 D-Mannose 6-phosphate
-
 Bacillus amyloliquefaciens DSM 7 D-Fructose 6-phosphate
-
 ?
5.3.1.8 D-ribose poor substrate Bacillus amyloliquefaciens D-ribulose
-
 ?
5.3.1.8 D-ribose poor substrate Bacillus amyloliquefaciens DSM 7 D-ribulose
-
 ?
5.3.1.8 L-ribulose poor substrate Bacillus amyloliquefaciens L-ribose
-
 ?
5.3.1.8 L-ribulose poor substrate Bacillus amyloliquefaciens DSM 7 L-ribose
-
 ?
5.3.1.8 L-talose poor substrate Bacillus amyloliquefaciens L-tagatose
-
 ?
5.3.1.8 L-talose poor substrate Bacillus amyloliquefaciens DSM 7 L-tagatose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.8 monomer 1 * 35490, calculated from sequence, 1 * 35000, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
5.3.1.8 M6PI
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.8 70
-
-
 Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.8 40
-
 presence of Co2+, half-life 15 h Bacillus amyloliquefaciens
5.3.1.8 50
-
 presence of Co2+, half-life 3.5 h Bacillus amyloliquefaciens
5.3.1.8 60
-
 presence of Co2+, half-life 10 min Bacillus amyloliquefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.8 106000
-
 D-mannose 6-phosphate pH 7.5, 70°C Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.8 7.5
-
-
 Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.8 4
-
 83% of maximum activity Bacillus amyloliquefaciens
5.3.1.8 9
-
 94% of maximum activity Bacillus amyloliquefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.8 13900
-
 D-mannose 6-phosphate pH 7.5, 70°C Bacillus amyloliquefaciens