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Literature summary extracted from

  • Dueholm, B.; Drew, D.P.; Sweetman, C.; Simonsen, H.T.
    In planta and in silico characterization of five sesquiterpene synthases from Vitis vinifera (cv. Shiraz) berries (2019), Planta, 249, 59-70 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.75 expressed in Nicotiana benthamiana Vitis vinifera
4.2.3.128 gene TPS-Y2, recombinant expression in Nicotiana benthamiana Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.75 (2E,6E)-farnesyl diphosphate Vitis vinifera
-
 (-)-germacrene D + alpha-ylangene + diphosphate
-
 ?
4.2.3.128 (2E,6E)-farnesyl diphosphate Vitis vinifera
-
 beta-cubebene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.75 Vitis vinifera Q6Q3H3 cultivar Shiraz
-
4.2.3.128 Vitis vinifera
-
 cv. Shiraz
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.75 fruit
-
 Vitis vinifera
-
4.2.3.128 fruit enzyme is isolated from ripe and immature berries Vitis vinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.75 (2E,6E)-farnesyl diphosphate
-
 Vitis vinifera (-)-germacrene D + alpha-ylangene + diphosphate
-
 ?
4.2.3.128 (2E,6E)-farnesyl diphosphate
-
 Vitis vinifera beta-cubebene + diphosphate
-
 ?
4.2.3.128 (2E,6E)-farnesyl diphosphate
-
 Vitis vinifera alpha-cubebene + diphosphate
-
 ?
4.2.3.128 additional information the enzyme also forms beta-caryophyllene via (Z,E)-germacradienyl cation and (E,E)-humulyl cation intermediates. The product profile of the VvShirazTPS-Y2 reaction includes alpha- and beta-cubebene, alpha-copaene, isocaryophyllene, alpha-caryophyllene, (+)-delta-cadinene, cadina-1,4-diene, and caryophyllene oxide, overview. The major products are isocaryophyllene and beta-cubenene Vitis vinifera ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.75 germacrene D synthase
-
 Vitis vinifera
4.2.3.75 TPS07
-
 Vitis vinifera
4.2.3.75 ylangene/germacrene synthase
-
 Vitis vinifera
4.2.3.128 caryophyllene/cubebene synthase
-
 Vitis vinifera
4.2.3.128 VvShirazTPS-Y2
-
 Vitis vinifera
4.2.3.128 VvTPS
-
 Vitis vinifera

General Information

EC Number General Information Comment Organism
4.2.3.128 metabolism the farnesyl cation can undergo either 1,10- or 1,11-cyclization, giving rise to either an (E,E)-germacradienyl cation or an (E,E)-humulyl cation, respectively Vitis vinifera
4.2.3.128 physiological function VvShirazTPSY2 is a caryophyllene/cubebene synthase Vitis vinifera