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Literature summary extracted from

  • Xu, B.; Lei, L.; Zhu, X.; Zhou, Y.; Xiao, Y.
    Identification and characterization of L-lysine decarboxylase from Huperzia serrata and its role in the metabolic pathway of lycopodium alkaloid (2017), Phytochemistry, 136, 23-30 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.B12 six cDNAs encoding lysine decarboxylases (LDCs) are cloned from Huperzia serrata by degenerate PCR and rapid amplification of cDNA ends (RACE), phylogenetic analysis, one HsLDC isoform is functionally characterized as lysine decarboxylase. Recombinant transient expression of HsLDC in Nicotiana tabacum leaves specifically increase cadaverine content from zero to 0.75 mg per gram of dry mass. Recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Huperzia serrata

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.B12 K118A site-directed mutagenesis, the mutant shows about 90% reduced activity compared to the wild-type enzyme Huperzia serrata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.B12 0.194
-
 L-methionine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.209
-
 L-arginine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.878
-
 L-lysine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 1.07
-
 L-norleucine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 1.32
-
 L-cysteine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 1.43
-
 L-ornithine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 1.64
-
 D-Lysine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 5.77
-
 L-valine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 15.7
-
 L-phenylalanine pH 7.5, 37°C, recombinant enzyme Huperzia serrata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.B12 L-lysine Huperzia serrata
-
 cadaverine + CO2
-
 ?
4.1.1.B12 L-ornithine Huperzia serrata
-
 putrescine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.B12 Huperzia serrata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.B12 D-lysine 3.0% cataytic efficiency compared to L-lysine Huperzia serrata cadaverine + CO2
-
 ?
4.1.1.B12 L-arginine 19.5% cataytic efficiency compared to L-lysine Huperzia serrata agmatine + CO2
-
 ?
4.1.1.B12 L-cysteine 5.5% cataytic efficiency compared to L-lysine Huperzia serrata ?
-
 ?
4.1.1.B12 L-lysine
-
 Huperzia serrata cadaverine + CO2
-
 ?
4.1.1.B12 L-lysine best substrate Huperzia serrata cadaverine + CO2
-
 ?
4.1.1.B12 L-methionine 21.0% cataytic efficiency compared to L-lysine Huperzia serrata 3-(methylsulfanyl)propanamine + CO2
-
 ?
4.1.1.B12 L-norleucine 4.5% cataytic efficiency compared to L-lysine Huperzia serrata ?
-
 ?
4.1.1.B12 L-ornithine
-
 Huperzia serrata putrescine + CO2
-
 ?
4.1.1.B12 L-ornithine 92% cataytic efficiency compared to L-lysine Huperzia serrata putrescine + CO2
-
 ?
4.1.1.B12 L-phenylalanine 1.2% cataytic efficiency compared to L-lysine Huperzia serrata phenylethylamine + CO2
-
 ?
4.1.1.B12 L-valine 1.8% cataytic efficiency compared to L-lysine Huperzia serrata 2-methylpropanamine + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.B12 HsLDC
-
 Huperzia serrata
4.1.1.B12 L-lysine decarboxylase
-
 Huperzia serrata
4.1.1.B12 LDC
-
 Huperzia serrata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.B12 37
-
 assay at Huperzia serrata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.B12 0.0859
-
 L-methionine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.0859
-
 L-arginine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.102
-
 L-norleucine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.102
-
 D-Lysine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.155
-
 L-cysteine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.214
-
 L-valine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.404
-
 L-phenylalanine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 1.85
-
 L-lysine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 2.78
-
 L-ornithine pH 7.5, 37°C, recombinant enzyme Huperzia serrata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.B12 7.5
-
 assay at Huperzia serrata

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.B12 pyridoxal 5'-phosphate
-
 Huperzia serrata

General Information

EC Number General Information Comment Organism
4.1.1.B12 metabolism the enzyme plays a role in the metabolic pathway of lycopodium alkaloid. Lysine decarboxylation is the first biosynthetic step of Huperzine A (HupA) Huperzia serrata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.B12 0.0257
-
 L-phenylalanine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.0371
-
 L-valine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.0625
-
 D-Lysine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.0957
-
 L-norleucine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.117
-
 L-cysteine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.412
-
 L-arginine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 0.442
-
 L-methionine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 1.94
-
 L-ornithine pH 7.5, 37°C, recombinant enzyme Huperzia serrata
4.1.1.B12 2.11
-
 L-lysine pH 7.5, 37°C, recombinant enzyme Huperzia serrata