Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rodriguez-Bolanos, M.; Cabrera, N.; Perez-Montfort, R.
    Identification of the critical residues responsible for differential reactivation of the triosephosphate isomerases of two trypanosomes (2016), Open biology, 6, 160161 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.3.1.1 analysis systematic mutagenesis method to find critical residues for certain physico-chemical properties of a protein. The method is a shorter alternative to random mutagenesis, saturation mutagenesis or directed evolution to find multiple amino acids critical for certain properties of proteins Trypanosoma brucei brucei
5.3.1.1 analysis systematic mutagenesis method to find critical residues for certain physico-chemical properties of a protein. The method is a shorter alternative to random mutagenesis, saturation mutagenesis or directed evolution to find multiple amino acids critical for certain properties of proteins Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.1 additional information construction of mutants for interconversion of the behavior of the enzymes from Trypanosoma brucei brucei and Trypanosoma cruzi. To make Trypanosoma cruzi TIM reactivate with a Trypanosoma brucei brucei TIM-like behaviour you need to mutate the following amino acids: 18, 19, 20, 22, 23 and 26, and R2: 43, 46, 48, 49, 53, 56 and 57. To make a Trypanosoma brucei brucei TIM reactivate with a Trypanosoma cruzi TIM-like behaviour you need to mutate amino acids 18, 23, 26, 32, 33 and 34, and 43, 46, 48, 49, 53, 56 and 57 Trypanosoma brucei brucei
5.3.1.1 additional information construction of mutants for interconversion of the behavior of the enzymes from Trypanosoma brucei brucei and Trypanosoma cruzi. To make Trypanosoma cruzi TIM reactivate with a Trypanosoma brucei brucei TIM-like behaviour you need to mutate the following amino acids: 18, 19, 20, 22, 23 and 26, and R2: 43, 46, 48, 49, 53, 56 and 57. To make a Trypanosoma brucei brucei TIM reactivate with a Trypanosoma cruzi TIM-like behaviour you need to mutate amino acids 18, 23, 26, 32, 33 and 34, and 43, 46, 48, 49, 53, 56 and 57 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 0.44
-
 D-glyceraldehyde 3-phosphate wild-type, 25°C, pH not specified in the publication Trypanosoma brucei brucei
5.3.1.1 0.45
-
 D-glyceraldehyde 3-phosphate wild-type, 25°C, pH not specified in the publication Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.1.1 glycosome
-
 Trypanosoma cruzi 20015
-

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Trypanosoma brucei brucei P04789
-
-
5.3.1.1 Trypanosoma cruzi P52270
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-glyceraldehyde 3-phosphate
-
 Trypanosoma brucei brucei dihydroxyacetone 3-phosphate
-
 ?
5.3.1.1 D-glyceraldehyde 3-phosphate
-
 Trypanosoma cruzi dihydroxyacetone 3-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.1 5167
-
 D-glyceraldehyde 3-phosphate wild-type, 25°C, pH not specified in the publication Trypanosoma cruzi
5.3.1.1 6000
-
 D-glyceraldehyde 3-phosphate wild-type, 25°C, pH not specified in the publication Trypanosoma brucei brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.1 11500
-
 D-glyceraldehyde 3-phosphate wild-type, 25°C, pH not specified in the publication Trypanosoma cruzi
5.3.1.1 13600
-
 D-glyceraldehyde 3-phosphate wild-type, 25°C, pH not specified in the publication Trypanosoma brucei brucei