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Literature summary extracted from

  • Maderbocus, R.; Fields, B.L.; Hamilton, K.; Luo, S.; Tran, T.H.; Dietrich, L.E.P.; Tong, L.
    Crystal structure of a Pseudomonas malonate decarboxylase holoenzyme hetero-tetramer (2017), Nat. Commun., 8, 160 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.88 additional information the enzyme is independent from biotin Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.88 expressed in Escherichia coli BL21Star (DE3), BL21 Rosetta, and B834 (DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.88 MdcD-MdcE subunits, sitting drop vapor diffusion method, using 0.1 M Bis-Tris (pH 5.8), 18% (w/v) PEG 3350, and 0.2 M ammonium acetate. Holoenzyme in complex with CoA and malonate, sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 8% (w/v) tacsimate Pseudomonas aeruginosa
4.1.1.88 the holoenzyme contains four subunits, having an acyl-carrier protein (MdcC subunit) with a distinct prosthetic group, as well as decarboxylase (MdcD-MdcE) and acyl-carrier protein transferase (MdcA) catalytic activities. Structure of the MdcD-MdcE complex at 1.86 A resolution, and overall strcuture of the heterotetramer at 2.2 A resolution Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.88 Q100E the mutant of subunit MdcE has a roughly 10fold lower catalytic activity compared to the wild type enzyme Pseudomonas aeruginosa
4.1.1.88 Q100E/Y102F inactive Pseudomonas aeruginosa
4.1.1.88 S142A the mutant of subunit MdcE has a roughly 10fold lower catalytic activity compared to the wild type enzyme Pseudomonas aeruginosa
4.1.1.88 Y102F the mutant of subunit MdcE has about wild type catalytic activity Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.88 membrane
-
 Pseudomonas aeruginosa 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.88 140000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.88 malonate + H+ Pseudomonas aeruginosa
-
 acetate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.88 Pseudomonas aeruginosa Q9I6S7 and Q9I6S6 beta subunit MdcD and gamma subunit MdcE
-
4.1.1.88 Pseudomonas aeruginosa Q9I6T0 and Q9I6S6 Q9I6T0 i.e. subunit MdcA, Q9I6S6 i.e. subunit MdcE
-
4.1.1.88 Pseudomonas aeruginosa DSM 22644 Q9I6T0 and Q9I6S6 Q9I6T0 i.e. subunit MdcA, Q9I6S6 i.e. subunit MdcE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.88 nickel bead column chromatography and Sephacryl S-300 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.88 malonate + H+
-
 Pseudomonas aeruginosa acetate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.88 heterotetramer x-ray crystallography Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
4.1.1.88 biotin-independent malonate decarboxylase the holoenzyme contains four subunits having an acyl-carrier protein (MdcC subunit) with a distinct prosthetic group, as well as decarboxylase (MdcD-MdcE) and acyl-carrier protein transferase (MdcA) catalytic activities Pseudomonas aeruginosa
4.1.1.88 MdcACDE
-
 Pseudomonas aeruginosa