BRENDA - Enzyme Database

Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis

Wiktor, M.; Weichert, D.; Howe, N.; Huang, C.Y.; Olieric, V.; Boland, C.; Bailey, J.; Vogeley, L.; Stansfeld, P.J.; Buddelmeijer, N.; Wang, M.; Caffrey, M.; Nat. Commun. 8, 15952 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.269
expressed in Escherichia coli C41(DE3) cells
Escherichia coli
2.3.1.269
expressed in Escherichia coli C41(DE3) cells
Pseudomonas aeruginosa
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.3.1.269
using 30% (w/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate
Pseudomonas aeruginosa
2.3.1.269
using 8% (w/v) 2-methyl-2,4-pentanediol, 0.1 M MES pH 6.0 and 0.4 M ammonium citrate
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.269
C387A
inactive
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.269
membrane
-
Escherichia coli
16020
-
2.3.1.269
membrane
-
Pseudomonas aeruginosa
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.269
Escherichia coli
P23930
-
-
2.3.1.269
Pseudomonas aeruginosa
Q9ZI86
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.269
Ni-NTA column chromatography and Superdex 200 gel filtration
Escherichia coli
2.3.1.269
Ni-NTA column chromatography and Superdex 200 gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.269
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
748746
Escherichia coli
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
2.3.1.269
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
748746
Pseudomonas aeruginosa
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.269
expressed in Escherichia coli C41(DE3) cells
Escherichia coli
2.3.1.269
expressed in Escherichia coli C41(DE3) cells
Pseudomonas aeruginosa
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.3.1.269
using 30% (w/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate
Pseudomonas aeruginosa
2.3.1.269
using 8% (w/v) 2-methyl-2,4-pentanediol, 0.1 M MES pH 6.0 and 0.4 M ammonium citrate
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.269
C387A
inactive
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.269
membrane
-
Escherichia coli
16020
-
2.3.1.269
membrane
-
Pseudomonas aeruginosa
16020
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.269
Ni-NTA column chromatography and Superdex 200 gel filtration
Escherichia coli
2.3.1.269
Ni-NTA column chromatography and Superdex 200 gel filtration
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.269
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
748746
Escherichia coli
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?
2.3.1.269
phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
748746
Pseudomonas aeruginosa
1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
-
-
?