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Literature summary extracted from

  • Wiktor, M.; Weichert, D.; Howe, N.; Huang, C.Y.; Olieric, V.; Boland, C.; Bailey, J.; Vogeley, L.; Stansfeld, P.J.; Buddelmeijer, N.; Wang, M.; Caffrey, M.
    Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis (2017), Nat. Commun., 8, 15952 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.269 expressed in Escherichia coli C41(DE3) cells Escherichia coli
2.3.1.269 expressed in Escherichia coli C41(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.269 using 30% (w/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate Pseudomonas aeruginosa
2.3.1.269 using 8% (w/v) 2-methyl-2,4-pentanediol, 0.1 M MES pH 6.0 and 0.4 M ammonium citrate Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.269 C387A inactive Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.269 membrane
-
 Escherichia coli 16020
-
2.3.1.269 membrane
-
 Pseudomonas aeruginosa 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.269 Escherichia coli P23930
-
-
2.3.1.269 Pseudomonas aeruginosa Q9ZI86
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.269 Ni-NTA column chromatography and Superdex 200 gel filtration Escherichia coli
2.3.1.269 Ni-NTA column chromatography and Superdex 200 gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.269 phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
 Escherichia coli 1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
 ?
2.3.1.269 phosphatidylethanolamine + [apolipoprotein FSL-1]-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
 Pseudomonas aeruginosa 1-lyso-phosphatidylethanolamine + [lipoprotein FSL-1]-N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.269 lnt
-
 Escherichia coli
2.3.1.269 lnt
-
 Pseudomonas aeruginosa